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Absence of Glial Fibrillary Acidic Protein and Vimentin Prevents Hypertrophy of Astrocytic Processes and Improves Post-Traumatic Regeneration
Open accessAbstract
The regenerative capacity of the CNS is extremely limited. The reason for this is unclear, but glial cell involvement has been suspected, and oligodendrocytes have been implicated as inhibitors of neuroregeneration (Chen et al., 2000, GrandPre et al., 2000; Fournier et al., 2001). The role of astrocytes in this process was proposed but remains incompletely understood (Silver and Miller, 2004). Astrocyte activation (reactive gliosis) accompanies neurotrauma, stroke, neurodegenerative diseases, or tumors. Two prominent hallmarks of reactive gliosis are hypertrophy of astrocytic processes and upregulation of intermediate filaments. Using the entorhinal cortex lesion model in mice, we found that reactive astrocytes devoid of the intermediate filament proteins glial fibrillary acidic protein and vimentin (GFAP-/-Vim-/-), and consequently lacking intermediate filaments (Colucci-Guyon et al., 1994; Pekny et al., 1995; Eliasson et al., 1999), showed only a limited hypertrophy of cell processes. Instead, many processes were shorter and not straight, albeit the volume of neuropil reached by a single astrocyte was the same as in wild-type mice. This was accompanied by remarkable synaptic regeneration in the hippocampus. On a molecular level,GFAP-/-Vim-/-reactive astrocytes could not upregulate endothelin B receptors, suggesting that the upregulation is intermediate filament dependent. These findings show a novel role for intermediate filaments in astrocytes and implicate reactive astrocytes as potent inhibitors of neuroregeneration.
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“Under baseline conditions, however, Gfap−/−Vim−/− mice have astrocytes of normal appearance and grossly normal brain anatomy (22, 23, 32).”
From Attenuating astrocyte activation accelerates plaque pathogenesis in APP/PS1 mice (2013) · cites this paper as [23]
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“The intermediate filament proteins GFAP and Vim are expressed at high levels in activated astrocytes and are critical for the activated phenotype.”
From Attenuating astrocyte activation accelerates plaque pathogenesis in APP/PS1 mice (2013) · cites this paper as [23]
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“But when Gfap and Vim are both deleted, astrocytes cannot form intermediate filament networks or display reactive morphology (22, 23, 32).”
From Attenuating astrocyte activation accelerates plaque pathogenesis in APP/PS1 mice (2013) · cites this paper as [23]
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“On a cellular level, astrocytes of Gfap−/−Vim−/− mice in the injured area do not develop the characteristic hypertrophy of proximal processes (23).”
From Attenuating astrocyte activation accelerates plaque pathogenesis in APP/PS1 mice (2013) · cites this paper as [23]
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“Interestingly, Gfap−/−Vim−/− mice exhibit better posttraumatic regeneration of neuronal synapses (23) and axons (34), increased baseline and posttraumatic neurogenesis (35), and improved functional recovery after spinal cord trauma (36).”
From Attenuating astrocyte activation accelerates plaque pathogenesis in APP/PS1 mice (2013) · cites this paper as [23]
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“In several models of acute central nervous system injury, deletion of the genes encoding these proteins suppresses morphological changes associated with astrocyte activation (22, 23, 26, 30, 32, 36).”
From Attenuating astrocyte activation accelerates plaque pathogenesis in APP/PS1 mice (2013) · cites this paper as [23]
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Citations
Cites
A new mathematical model for relative quantification in real-time RT-PCR
Pfaffl, M. W. (2001) · Nucleic Acids Research · doi:10.1093/nar/29.9.e45 · Cited by 34,610
Antibodies, a laboratory manual
(1989) · Biomedicine & Pharmacotherapy · doi:10.1016/0753-3322(89)90130-3 · Cited by 12,318
Bustin, S. A. (2000) · Journal of Molecular Endocrinology · doi:10.1677/jme.0.0250169 · Cited by 3,844
Embedding in Epoxy Resins for Ultrathin Sectioning in Electron Microscopy
Richardson, K. C., Jarett, L., & Finke, E. H. (1960) · Stain Technology · doi:10.3109/10520296009114754 · Cited by 3,310
Regeneration beyond the glial scar
Silver, J., & Miller, J. H. (2004) · Nature reviews. Neuroscience · doi:10.1038/nrn1326 · Cited by 3,151
Ischemic Cell Death in Brain Neurons
Lipton, P. (1999) · Physiological Reviews · doi:10.1152/physrev.1999.79.4.1431 · Cited by 3,043
Thresholds in cerebral ischemia - the ischemic penumbra.
Astrup, J., Siesjö, B. K., & Symon, L. (1981) · Stroke · doi:10.1161/01.str.12.6.723 · Cited by 2,820
The blood–brain barrier: an overview
Ballabh, P., Braun, A., & Nedergaard, M. (2004) · Neurobiology of Disease · doi:10.1016/j.nbd.2003.12.016 · Cited by 2,276
Glutamate-mediated astrocyte–neuron signalling
Parpura, V., Basarsky, T. A., Liu, F., Jeftinija, K., Jeftinija, S., & Haydon, P. G. (1994) · Nature · doi:10.1038/369744a0 · Cited by 1,768
Reactive astrocytes: cellular and molecular cues to biological function
Ridet, J., Privat, A., Malhotra, S. K., & Gage, F. H. (1997) · Trends in Neurosciences · doi:10.1016/s0166-2236(97)01139-9 · Cited by 1,766
Research works printed but not linked: 5
- 1999, J Biol Chem, 274, 3996
- 2000, Invest Ophthalmol Vis Sci, 41, 4268
- 2001, Prog Brain Res, 132, 23
- 1969, Stain Technol, 35, 313
- 1997, Trends Neurosci, 12, 570
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Lenzer, J. (2014) · CUNY Academic Works (City University of New York)
Glial cell reactivity in mouse models reflecting different aspects of Alzheimer's disease
Behrendt, M. G. (2012) · Electronic Theses of LMU Munich (Ludwig-Maximilians-Universität München) · doi:10.5282/edoc.14767
Regulation of neural stem cells during development and regeneration of the central nervous system
Fukuda, S., & Taga, T. (2005) · Inflammation and Regeneration · doi:10.2492/jsir.25.18
Somatostatin Receptors on Neuronal Cilia: Evidence for Neuroprotection
Evans, S. K. (2012) · University of North Texas Digital Library (University of North Texas) · doi:10.12794/metadc177195
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Zhang, J. L. (2009) · Gothenburg University Publications Electronic Archive (Gothenburg University)
Andersson, H. C. (2011) · Gothenburg University Publications Electronic Archive (Gothenburg University)
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Hultman, K. (2010) · Gothenburg University Publications Electronic Archive (Gothenburg University)
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Nodin, C. (2008) · Gothenburg University Publications Electronic Archive (Gothenburg University)
Ando, T., Obara, M., Sato, S., Ashida, H., Toyooka, T., Kobayashi, H., & Nawashiro, H. (2011) · International Symposium on Access Spaces (ISAS) · doi:10.1109/isas.2011.5960940
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Moeton, M. (2014) · UvA-DARE (University of Amsterdam)
Lončarević‐Vasiljković, N., Pešić, V., Tanić, N., Milanović, D., Mladenović, A., Perović, M., Kanazir, S., & Ruždijić, S. (2013) · Archives of Biological Sciences · doi:10.2298/abs1301255l
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Ando, T., Sato, S., Toyooka, T., Kobayashi, H., Nawashiro, H., Ashida, H., & Obara, M. (2012) · Nippon Laser Igakkaishi · doi:10.2530/jslsm.33.97
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Hubbard, J. A., & Binder, D. K. (2016) · Elsevier eBooks · doi:10.1016/b978-0-12-802401-0.00005-3
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Buchli, A. D., & Schwab, M. E. (2012) · Oxford University Press eBooks · doi:10.1093/med/9780199794591.003.0056
Richardson, R. M., & Bullock, M. R. (2011) · Elsevier eBooks · doi:10.1016/b978-1-4160-5316-3.00332-4
Lončarević‐Vasiljković, N. (2012) · doi:10.2298/bg20120928loncarevic
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Wizeman, J. W. (2016) · OpenCommons - UConn (University of Connecticut)
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Rosenthal, Z. P., Kraft, A. W., Czerniewski, L. R., & Lee, J. (2018) · Elsevier eBooks · doi:10.1016/b978-0-12-809813-4.00005-3
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Jurado, E. M. B. (2020) · Repositorio Institucional (Pontificia Universidad Javeriana) · doi:10.11144/javeriana.10554.41814
dos Santos, U. R. (2022) · Digital Library of Theses and Dissertations (Universidade de São Paulo) · doi:10.11606/d.109.2022.tde-08112022-084835
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Zhu, Y., Yue, C., Yang, Q., Zhang, Z., & Xu, X. (2023) · Indian Journal of Pharmaceutical Sciences · doi:10.36468/pharmaceutical-sciences.spl.785
Patel, B., & Acharya, N. S. (2024) · Journal of Young Pharmacists · doi:10.5530/jyp.2024.16.22
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Megemont, M. (2019) · theses.fr (ABES) · doi:10.70675/c0dcb017z7bb2z46e9zb39azc730e2593a65
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Clément, T. (2020) · theses.fr (ABES) · doi:10.70675/2892171fzc838z4d9azbf65zf208dafdf173
Bringuier, C. M. (2020) · theses.fr (ABES) · doi:10.70675/e40cbaaazaec8z46aazaf4fz41b8415fd687
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Provenzano, G. D. (2021) · theses.fr (ABES) · doi:10.70675/dcc9ac33zf0b2z45b9zbd23z37f03e91208c
Karakoyun, C., Virhammar, J., Rostami, E., Zetterberg, H., & Kumlien, E. (2026) · Acta Neurologica Scandinavica · doi:10.1155/ane/6656280
GFAP mutants cause imbalance of selective lipid classes in a cellular model of Alexander disease
Fernàndez‐Bernal, A., Goya‐Iglesias, N., Mota‐Martorell, N., Pekna, M., Pekny, M., Sol, J., Obis, È., Pamplona, R., Pajares, M. A., Pérez‐Sala, D., et al. (2026) · iScience · doi:10.1016/j.isci.2026.117903
Self-citationMolecular Characterization of Experimental Traumatic Brain Injury
Israelsson, C. (2006) · KTH Publication Database DiVA (KTH Royal Institute of Technology)
Metadata
- Authors
- Wilhelmsson, U., Li, L., Pekna, M., Berthold, C.-H., Blom, S., Eliasson, C., Renner, O., Bushong, E., Ellisman, M., Morgan, T. E., & Pekny, M.
- Affiliations
- University of Gothenburg (SE)
- Spanish National Cancer Research Centre (ES)
- University of California San Diego (US)
- University of Southern California (US)
- ORCID
- Venue
- The Journal of Neuroscience
- Publisher
- Society for Neuroscience
- Published
- 2004-05-26
- Volume
- 24
- Issue
- 21
- Pages
- 5016-5021
- ISSN
- 0270-6474 (ISSN-L)
- 1529-2401
- PMC
- PMC6729371
- OpenAlex
- W2166851454
- Cited by
- 455
- References
- 126
- Citations by year
- 2026: 14 · 2025: 13 · 2024: 14 · 2023: 12 · 2022: 24 · 2021: 23 · 2020: 31 · 2019: 22 · 2018: 15 · 2017: 18 · 2016: 15 · 2015: 24 · 2014: 26 · 2013: 27 · 2012: 36
- FWCI
- 14.56
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Journal of Neuroscience (publishedVersion, cc-by-nc-sa)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Gothenburg University Publications Electronic Archive (Gothenburg University) (submittedVersion)
- Funders
- Alzheimer's Association
- Swedish Cancer Foundation
- Svenska Sällskapet för Medicinsk Forskning
- Vetenskapsrådet
- National Institutes of Health (RR 04050)
- National Center for Research Resources (RR 04050, P41RR004050, P41 RR04050)
- Topics
- Life Sciences › Neuroscience › Neurology › Neuroinflammation and Neurodegeneration Mechanisms
- Life Sciences › Neuroscience › Developmental Neuroscience › Neurogenesis and neuroplasticity mechanisms
- Health Sciences › Medicine › Clinical Neurology › Traumatic Brain Injury and Neurovascular Disturbances
- Keywords
- glial fibrillary acidic protein, vimentin, intermediate filaments, reactive astrocytes, astrocyte hypertrophy, astrocytic processes, nerve regeneration, entorhinal cortex lesion, endothelin B receptor, synaptic regeneration, hippocampus
- MeSH terms
- Animals, Astrocytes, Brain Injuries, Cells, Cultured, Cytoplasm, Glial Fibrillary Acidic Protein, Glutamate-Ammonia Ligase, Hippocampus, Hypertrophy, Mice, Inbred C57BL, Nerve Regeneration, Vimentin, Up-Regulation, Entorhinal Cortex, Receptor, Endothelin B, Mice
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