Removal of AMPA receptors (AMPARs) from synapses is preceded by transient endocytosis of extrasynaptic AMPARs
Open accessAbstract
AMPA receptors (AMPARs) are dynamically regulated at synapses, but the time course and location of their exocytosis and endocytosis are not known. Therefore, we have used ecliptic pHluorin-tagged glutamate receptor 2 to visualize changes in AMPAR surface expression in real time. We show that synaptic and extrasynaptic AMPARs respond very differently to NMDA receptor activation; there is a rapid internalization of extrasynaptic AMPARs that precedes the delayed removal of synaptic AMPARs.
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SEP fluorescence is quenched in acidified endosomal compartments but not in the extracellular environment after incorporation into the plasma membrane.
“whose fluorescence is quenched in the acidified endosomal compartments, but not in the extracellular environment after incorporation into the plasma membrane.”
From Barriers in the brain: resolving dendritic spine morphology and compartmentalization (2014) · cites this paper as Ashby et al., 2004
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References 23 · Cited by 244
Research works printed but not linked: 18
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- Authors
- Ashby, M. C., De La Rue, S. A., Ralph, G. S., Uney, J., Collingridge, G. L., & Henley, J. M.
- Affiliations
- University of Bristol (GB)
- Venue
- The Journal of Neuroscience
- Publisher
- Society for Neuroscience
- Published
- 2004-06-02
- Volume
- 24
- Issue
- 22
- Pages
- 5172-5176
- ISSN
- 0270-6474 (ISSN-L)
- 1529-2401
- PMC
- PMC3309030
- OpenAlex
- W2146333273
- Cited by
- 244
- References
- 23
- Citations by year
- 2026: 5 · 2025: 5 · 2024: 5 · 2023: 3 · 2022: 7 · 2021: 8 · 2020: 8 · 2019: 6 · 2018: 10 · 2017: 6 · 2016: 8 · 2015: 5 · 2014: 18 · 2013: 13 · 2012: 17
- FWCI
- 7.16
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Journal of Neuroscience (publishedVersion, cc-by-nc-sa)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Bristol Research (University of Bristol) (publishedVersion, other-oa)
- Explore Bristol Research (submittedVersion, other-oa)
- Funders
- Wellcome Trust (61600, 069280)
- European Commission
- Medical Research Council
- Topics
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Receptor Mechanisms and Signaling
- Keywords
- AMPA receptors, receptor internalization, endocytosis, NMDA receptor activation, pHluorin, receptor surface expression, synaptic plasticity
- MeSH terms
- Animals, Cell Membrane, Cells, Cultured, Endocytosis, Hippocampus, Hydrogen-Ion Concentration, Luminescent Proteins, Microscopy, Fluorescence, Neurons, Recombinant Fusion Proteins, Synapses, Receptors, N-Methyl-D-Aspartate, Receptors, AMPA, Protein Transport, Green Fluorescent Proteins, Rats
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