Cited by 342Neuroinflammation and Neurodegeneration MechanismsTraumatic Brain Injury and Neurovascular DisturbancesSpinal Cord Injury Research

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Reactive astrogliosis in the neonatal mouse brain and its modulation by cytokines

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Balasingam, V, Tejada-Berges, T, Wright, E, Bouckova, R, Yong, VW·1994·The Journal of Neuroscience

DOI 10.1523/jneurosci.14-02-00846.1994PMC PMC6576825

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Abstract

Reactive astrogliosis is a characteristic response of astrocytes to inflammation and trauma of the adult CNS. To assess the hypothesis that cytokines from inflammatory mononuclear cells that accumulate around lesion sites have a role in modulating astrogliosis, this study sought to take advantage of the neonatal system in which astrogliosis is reported to be minimal following injury and in which the immune system is relatively immature compared to adult animals. A nitrocellulose membrane implant into the cortex of postnatal day 3 mice resulted in a tremendous astrogliotic response 4 d later, as measured by glial fibrillary acidic protein (GFAP) immunoreactivity and GFAP content. In contrast, a neonatal stab wound produced limited astroglial response when compared to the adult stab wound. Utilizing the neonatal stab wound model, cytokines were microinjected into the wound site at the time of injury. All cytokines tested (gamma-IFN, IL-1, IL-2, IL-6, TNF- alpha, and M-CSF) resulted in a significantly increased astrogliosis. The specificity of the cytokine response was demonstrated by the inability of human gamma-IFN, but not mouse gamma-IFN, in enhancing neonatal mouse astrogliosis, in accordance with reports that the interaction of gamma-IFN with its receptor occurs in a species-specific manner. We conclude that neonatal astrocytes can become reactive if an adequate injury stimulus is presented, and that the release of immunoregulatory cytokines by cells around lesion sites may be a mechanism that contributes to the production of gliosis.

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Authors: Balasingam, V, Tejada-Berges, T., Wright, E., Bouckova, R., & Yong, V.
Affiliations: McGill University (CA)
Venue: The Journal of Neuroscience
Publisher: Society for Neuroscience
Published: 1994-02-01
Volume: 14
Issue: 2
Pages: 846-856
ISSN: 0270-6474 (ISSN-L)
1529-2401
DOI: 10.1523/jneurosci.14-02-00846.1994
PMC: PMC6576825
OpenAlex: W2158406932
Cited by: 342
References: 63
Citations by year: 2026: 1 · 2025: 6 · 2024: 3 · 2023: 6 · 2022: 4 · 2021: 6 · 2020: 6 · 2019: 7 · 2018: 7 · 2017: 8 · 2016: 5 · 2015: 6 · 2014: 6 · 2013: 14 · 2012: 7
FWCI: 13.60
Type: journal article
Language: en
Open access: hybrid
doi.org
Licence: cc-by-nc-sa
creativecommons.org/licenses/by-nc-sa/4.0/ (vor, 181 d)
Locations: Journal of Neuroscience (publishedVersion, cc-by-nc-sa)
PubMed Central (publishedVersion)
PubMed (publishedVersion)
Funders: Government of Canada
Medical Research Council Canada
Medical Research Council
Topics: Life Sciences › Neuroscience › Neurology › Neuroinflammation and Neurodegeneration Mechanisms
Health Sciences › Medicine › Clinical Neurology › Traumatic Brain Injury and Neurovascular Disturbances
Health Sciences › Medicine › Clinical Neurology › Spinal Cord Injury Research
Keywords: reactive astrogliosis, neonatal brain, cytokines, astrocytes, stab wounds, glial fibrillary acidic protein, interferon-gamma, neuroinflammation, neonatal mice
MeSH terms: Animals, Animals, Newborn, Astrocytes, Brain, Brain Injuries, Cell Division, DNA, Female, Glial Fibrillary Acidic Protein, Humans, Inflammation, Interferon-gamma, Interleukin-1, Interleukin-2, Male, Mice, Inbred Strains, Microinjections, Recombinant Proteins, Thymidine, Time Factors, Tumor Necrosis Factor-alpha, Wounds, Stab, Biomarkers, Interleukin-6, Macrophage Colony-Stimulating Factor, Cytokines, Mice

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Reactive astrogliosis in the neonatal mouse brain and its modulation by cytokines

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