Characterization of a CNS Cell Line, CAD, in which Morphological Differentiation Is Initiated by Serum Deprivation
Open accessAbstract
A CNS catecholaminergic cell line, Cath.a, was established by targeted oncogenesis in transgenic mice. Cath.a cells express neuronal properties but lack neuronal morphology. Here, we describe a variant of Cath.a, called CAD (Cath.a-differentiated), in which reversible morphological differentiation can be initiated by removal of serum or exogenously added protein from the medium. In serum- or protein-free media, CAD cells stop proliferating and extend long processes. Differentiated CAD cells can be maintained without serum or protein for at least 6 weeks. CAD cells are distinct from Cath.a cells; most significant, the original immortalizing oncogene, SV40 T antigen, was spontaneously lost. By immunostaining or immunoblotting, we show that CAD cells express neuron-specific proteins, such as class III β-tubulin, GAP-43, SNAP-25, and synaptotagmin, but not GFAP. Ultrastructurally, processes from differentiated CAD cells have abundant parallel microtubules and intermediate filaments, and bear varicosities that contain both large dense-core vesicles/granules (120–160 nm) and smaller clear vesicles (60–80 nm). Additionally, CAD cells express enzymatically active tyrosine hydroxylase and accumulate l -DOPA. CAD cells exhibit biochemical and morphological characteristics of primary neurons and provide an unique tool for studying neuronal differentiation.
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CAD cells are a mouse catecholaminergic neuronal cell line.
“We first examined Cath.a-differentiated (CAD) cells, a mouse catecholaminergic neuronal cell line (56).”
From Regulation of cargo exocytosis by a Reps1-Ralbp1-RalA module (2023) · cites this paper as [56]
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Regulation of cargo exocytosis by a Reps1-Ralbp1-RalA module
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- Authors
- Qi, Y., Wang, J. K. T., McMillian, M., & Chikaraishi, D. M.
- Affiliations
- Tufts University (US)
- Duke Medical Center (US)
- ORCID
- Qi, Yanping
- Venue
- The Journal of Neuroscience
- Publisher
- Society for Neuroscience
- Published
- 1997-02-15
- Volume
- 17
- Issue
- 4
- Pages
- 1217-1225
- ISSN
- 0270-6474 (ISSN-L)
- 1529-2401
- PMC
- PMC6793738
- OpenAlex
- W1924764088
- Cited by
- 330
- References
- 0
- Citations by year
- 2026: 14 · 2025: 14 · 2024: 14 · 2023: 9 · 2022: 14 · 2021: 10 · 2020: 11 · 2019: 10 · 2018: 10 · 2017: 11 · 2016: 11 · 2015: 17 · 2014: 18 · 2013: 10 · 2012: 16
- FWCI
- 1.88
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Journal of Neuroscience (publishedVersion, cc-by-nc-sa)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health
- National Institute of Neurological Disorders and Stroke (R01NS022675, R01NS030590, NS30590, NS 22675)
- Topics
- Life Sciences › Neuroscience › Developmental Neuroscience › Neurogenesis and neuroplasticity mechanisms
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
- Keywords
- catecholaminergic neurons, neuronal differentiation, serum deprivation, neuron-specific proteins, TUBB3, GAP-43, SNAP-25, synaptotagmin, microtubules, intermediate filaments, varicosities, dense core vesicles, tyrosine hydroxylase, cell immortalization, transgenic mice
- MeSH terms
- Animals, Antigens, Polyomavirus Transforming, Brain Neoplasms, Catecholamines, Cell Differentiation, Cell Line, Transformed, Mice, Transgenic, Nerve Tissue Proteins, Neurons, Oncogenes, Tumor Cells, Cultured, Culture Media, Serum-Free, Mice
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