Arg-Gly-Asp-Ser-Selective Adhesion and the Stabilization of Long-Term Potentiation: Pharmacological Studies and the Characterization of a Candidate Matrix Receptor
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Peptides known to block the extracellular interactions of adhesion receptors belonging to a subclass of the integrin family were tested for their effects on the stabilization of long-term potentiation (LTP) in hippocampal slices. Theta burst stimulation delivered after infusions of Gly-Ala-Val-Ser-Thr-Ala (GAVSTA) resulted in a potentiation effect that decayed steadily over a period of 40 min; LTP elicited in the presence of inactive control peptides remained stable over this time period. GAVSTA had no detectible influence on baseline responses, induction processes, or the initial degree of potentiation. Infusions of integrin antagonists after application of theta bursts also resulted in the occurrence of a decremental form of LTP. Affinity chromatography was then used in an effort to identify targets of the structurally dissimilar integrin blockers that disrupt LTP stabilization. Both integrin antagonists Gly-Arg-Gly-Asp-Ser-Pro and GAVSTA eluted a major species of 55 kDa (synaptegrin-1) from GRGDSP-affinity columns that had been loaded with solubilized synaptic membranes; lesser concentrations of three polypeptides of ∼20, 27, and 30 kDa were also collected. Synaptegrin-1 was labeled by antibodies to the RGDS-binding integrin α 5 β 1. In addition, the synaptegrin, as well as the 27 kDa, protein was found to copurify with pre- and postsynaptic markers during the isolation of forebrain synaptosomes. These results indicate that a matrix recognition event occurring several minutes after induction of LTP is a necessary step in the stabilization of potentiated synapses; they also identify an integrin-like matrix receptor of 55 kDa that may contribute to this event.
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The RGD motif's role in recognition and binding to integrins and in plasticity and LTP is well documented.
“The RGD sequence is a short amino acid motif essential for cell adhesion. It is found in various cell adhesion molecules, and its importance in recognizing and binding to integrins and the role in plasticity and LTP have been widely documented (Bahr et al. 1997; Stäubli et al. 1998; Watson et al. 2007).”
From The Serine/Threonine Kinase <scp>NDR2</scp> Regulates Integrin Signaling, Synapse Formation, and Synaptic Plasticity in the Hippocampus (2025) · cites this paper as Bahr et al. 1997
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Research works printed but not linked: 3
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Proteases involved in long-term potentiation
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- Authors
- Bahr, B. A., Staubli, U., Xiao, P., Chun, D., Ji, Z.-X., Esteban, E. T., & Lynch, G.
- Affiliations
- University of California, Irvine (US)
- New York University (US)
- Venue
- The Journal of Neuroscience
- Publisher
- Society for Neuroscience
- Published
- 1997-02-15
- Volume
- 17
- Issue
- 4
- Pages
- 1320-1329
- ISSN
- 0270-6474 (ISSN-L)
- 1529-2401
- PMC
- PMC6793740
- OpenAlex
- W1600951028
- Cited by
- 165
- References
- 2
- Citations by year
- 2026: 1 · 2025: 1 · 2023: 2 · 2022: 2 · 2021: 1 · 2019: 2 · 2017: 4 · 2016: 3 · 2015: 1 · 2014: 4 · 2013: 3 · 2012: 3
- FWCI
- 5.38
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Journal of Neuroscience (publishedVersion, cc-by-nc-sa)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- York University
- Topics
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Health Sciences › Medicine › Immunology and Allergy › Cell Adhesion Molecules Research
- Life Sciences › Neuroscience › Neurology › Neuroinflammation and Neurodegeneration Mechanisms
- Keywords
- long-term potentiation, integrins, integrin antagonists, extracellular matrix receptors, hippocampus, synaptic adhesion, synaptosomes, alpha5beta1 integrin, synaptic plasticity
- MeSH terms
- Animals, Cell Adhesion, Electric Stimulation, Electrophysiology, Extracellular Matrix, Hippocampus, Male, Oligopeptides, Theta Rhythm, Integrins, Rats, Sprague-Dawley, Long-Term Potentiation, Rats, In Vitro Techniques
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