The DOI matches its Crossref record; 55 of the 55 references it cites were found in a registry or an index.
Neuronal P2X7Receptors Are Targeted to Presynaptic Terminals in the Central and Peripheral Nervous Systems
Open accessAbstract
The ionotropic ATP receptor subunits P2X(1-6) receptors play important roles in synaptic transmission, yet the P2X(7) receptor has been reported as absent from neurons in the normal adult brain. Here we use RT-PCR to demonstrate that transcripts for the P2X(7) receptor are present in extracts from the medulla oblongata, spinal cord, and nodose ganglion. Using in situ hybridization mRNA encoding, the P2X(7) receptor was detected in numerous neurons throughout the medulla oblongata and spinal cord. Localizing the P2X(7) receptor protein with immunohistochemistry and electron microscopy revealed that it is targeted to presynaptic terminals in the CNS. Anterograde labeling of vagal afferent terminals before immunohistochemistry confirmed the presence of the receptor in excitatory terminals. Pharmacological activation of the receptor in spinal cord slices by addition of 2'- and 3'-O-(4-benzoylbenzoyl)adenosine 5'-triphosphate (BzATP; 30 microm) resulted in glutamate mediated excitation of recorded neurons, blocked by P2X(7) receptor antagonists oxidized ATP (100 microm) and Brilliant Blue G (2 microm). At the neuromuscular junction (NMJ) immunohistochemistry revealed that the P2X(7) receptor was present in motor nerve terminals. Furthermore, motor nerve terminals loaded with the vital dye FM1-43 in isolated NMJ preparations destained after application of BzATP (30 microm). This BzATP evoked destaining is blocked by oxidized ATP (100 microm) and Brilliant Blue G (1 microm). This indicates that activation of the P2X(7) receptor promotes release of vesicular contents from presynaptic terminals. Such a widespread distribution and functional role suggests that the receptor may be involved in the fundamental regulation of synaptic transmission at the presynaptic site.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
In the citing paper
“Despite being detected in glial and neuronal cells [49,50,51,52,53,54], the activity of the P2X7R was not always investigated and used antibodies lack specificity [49,55,56,57].”
From P2X7 Receptor Signaling in Stress and Depression (2019) · cites this paper as [50]
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Receptors for Purines and Pyrimidines
Burnstock, G., & Verkhratsky, A. (2012) · Springer eBooks · doi:10.1007/978-3-642-28863-0_5 · Cited by 3,962
The Cytolytic P 2Z Receptor for Extracellular ATP Identified as a P 2X Receptor (P2X 7 )
Surprenant, A., Rassendren, F., Kawashima, E., North, R. A., & Buell, G. (1996) · Science · doi:10.1126/science.272.5262.735 · Cited by 1,739
Signal transduction via P2-purinergic receptors for extracellular ATP and other nucleotides
Dubyak, G., & El-Moatassim, C. (1993) · American Journal of Physiology-Cell Physiology · doi:10.1152/ajpcell.1993.265.3.c577 · Cited by 1,246
Collo, G., Ra, N., Kawashima, E. H., Merlo‐Pich, E., Neidhart, S., Surprenant, A., & Buell, G. N. (1996) · Journal of Neuroscience · doi:10.1523/jneurosci.16-08-02495.1996 · Cited by 876
The Role of Excitotoxicity in Neurodegenerative Disease
Doble, A. (1999) · Pharmacology & Therapeutics · doi:10.1016/s0163-7258(98)00042-4 · Cited by 723
Pharmacology of Cloned P2X Receptors
North, R. A., & Surprenant, A. (2000) · The Annual Review of Pharmacology and Toxicology · doi:10.1146/annurev.pharmtox.40.1.563 · Cited by 707
Activation of ATP P2X receptors elicits glutamate release from sensory neuron synapses
Gu, J. G., & MacDermott, A. B. (1997) · Nature · doi:10.1038/39639 · Cited by 494
Tissue distribution of the P2X7 receptor
Collo, G., Neidhart, S., Kawashima, E. H., Kosco‐Vilbois, M. H., North, R. A., & Buell, G. N. (1997) · Neuropharmacology · doi:10.1016/s0028-3908(97)00140-8 · Cited by 493
The Permeabilizing ATP Receptor, P2X7
Rassendren, F., Buell, G. N., Virginio, C., Collo, G., North, R. A., & Surprenant, A. (1997) · Journal of Biological Chemistry · doi:10.1074/jbc.272.9.5482 · Cited by 479
Extracellular ATP Triggers Tumor Necrosis Factor‐α Release from Rat Microglia
Hide, I., Tanaka, M., Inoue, A., Nakajima, K., Kohsaka, S., Inoue, K., & Nakata, Y. (2000) · Journal of Neurochemistry · doi:10.1046/j.1471-4159.2000.0750965.x · Cited by 447
Vulchanova, L., Riedl, M. S., Shuster, S. J., Buell, G. N., Surprenant, A., North, R. A., & Elde, R. (1997) · Neuropharmacology · doi:10.1016/s0028-3908(97)00126-3 · Cited by 417
Hetero-oligomeric Assembly of P2X Receptor Subunits
Torres, G. E., Egan, T. M., & Voigt, M. M. (1999) · Journal of Biological Chemistry · doi:10.1074/jbc.274.10.6653 · Cited by 414
Brilliant Blue G Selectively Blocks ATP-Gated Rat P2X7Receptors
Jiang, L., Mackenzie, A. B., North, R. A., & Surprenant, A. (2000) · Molecular Pharmacology · doi:10.1124/mol.58.1.82 · Cited by 401
MONITORING SECRETORY MEMBRANE WITH FM1-43 FLUORESCENCE
Cochilla, A. J., Angleson, J. K., & Betz, W. J. (1999) · Annual Review of Neuroscience · doi:10.1146/annurev.neuro.22.1.1 · Cited by 367
Virginio, C., MacKenzie, A., North, R. A., & Surprenant, A. (1999) · The Journal of Physiology · doi:10.1111/j.1469-7793.1999.0335m.x · Cited by 342
Pharmacological characterization of recombinant human and rat P2X receptor subtypes
Bianchi, B. R., Lynch, K. J., Touma, E., Niforatos, W., Burgard, E. C., Alexander, K. M., Park, H. S., Yu, H., Metzger, R. E., Kowaluk, E. A., et al. (1999) · European Journal of Pharmacology · doi:10.1016/s0014-2999(99)00350-7 · Cited by 331
Evans, R. J., Lewis, C. J., Buell, G. N., Valera, S., North, R. A., & Surprenant, A. (1995) · Molecular Pharmacology · doi:10.1016/s0026-895x(25)10232-0 · Cited by 316
Effects of divalent cations, protons and calmidazolium at the rat P2X7 receptor
Virginio, C., Church, D. J., North, R. A., & Surprenant, A. (1997) · Neuropharmacology · doi:10.1016/s0028-3908(97)00141-x · Cited by 313
ATP-mediated cytotoxicity in microglial cells
Ferrari, D., Chiozzi, P., Falzoni, S., Susino, M. D., Collo, G., Buell, G. N., & Virgilio, F. D. (1997) · Neuropharmacology · doi:10.1016/s0028-3908(97)00137-8 · Cited by 274
ATP P2XReceptors Mediate Fast Synaptic Transmission in the Dorsal Horn of the Rat Spinal Cord
Bardoni, R., Goldstein, P. A., Lee, C. J., Gu, J. G., & MacDermott, A. B. (1997) · Journal of Neuroscience · doi:10.1523/jneurosci.17-14-05297.1997 · Cited by 263
DISTRIBUTION OF P2X RECEPTORS IN THE URINARY BLADDER AND THE URETER OF THE RAT
Lee, H. Y., Bardini, M., & Burnstock, G. (2000) · The Journal of Urology · doi:10.1016/s0022-5347(05)67618-5 · Cited by 210
Pankratov, Y. V., Castro, E., Miras‐Portugal, M. T., & Krishtal, O. (1998) · European Journal of Neuroscience · doi:10.1046/j.1460-9568.1998.00419.x · Cited by 196
State-dependent cross-inhibition between transmitter-gated cation channels
Khakh, B. S., Zhou, X., Sydes, J., Galligan, J. J., & Lester, H. A. (2000) · Nature · doi:10.1038/35019066 · Cited by 188
P2X7 Receptors in Müller Glial Cells from the Human Retina
Pannicke, T., Fischer, W., Biedermann, B., Schädlich, H., Grosche, J., Faude, F., Wiedemann, P., Allgaier, C., Illés, P., Burnstock, G., et al. (2000) · Journal of Neuroscience · doi:10.1523/jneurosci.20-16-05965.2000 · Cited by 185
Rat astroglial P2Z (P2X7) receptors regulate intracellular calcium and purine release
Ballerini, P., Rathbone, M. P., Iorio, P. D., Renzetti, A., Giuliani, P., DʼAlimonte, L., Trubiani, O., Caciagli, F., & Ciccarelli, R. (1996) · Neuroreport · doi:10.1097/00001756-199611040-00026 · Cited by 149
Lê, K., Villeneuve, P., Ramjaun, A. R., McPherson, P. S., Beaudet, A., & Séguéla, P. (1998) · Neuroscience · doi:10.1016/s0306-4522(97)00365-5 · Cited by 148
Purines and their roles in apoptosis
Chow, S. C., Kass, G. E. N., & Orrenius, S. (1997) · Neuropharmacology · doi:10.1016/s0028-3908(97)00123-8 · Cited by 145
Role of ATP in fast excitatory synaptic potentials in locus coeruleus neurones of the rat
Nieber, K., Poelchen, W., & Illés, P. (1997) · British Journal of Pharmacology · doi:10.1038/sj.bjp.0701386 · Cited by 144
ATP Receptor-Mediated Enhancement of Fast Excitatory Neurotransmitter Release in the Brain
Khakh, B. S., & Henderson, G. (1998) · Molecular Pharmacology · doi:10.1124/mol.54.2.372 · Cited by 143
Voříšek, I., & Syková, E. (1997) · Journal of Cerebral Blood Flow & Metabolism · doi:10.1097/00004647-199702000-00009 · Cited by 142
ATP Stimulates Sympathetic Transmitter Release via Presynaptic P2X Purinoceptors
Boehm, S. (1999) · Journal of Neuroscience · doi:10.1523/jneurosci.19-02-00737.1999 · Cited by 140
Ultrastructural localization of P2X3receptors in rat sensory neurons
Llewellyn‐Smith, I., & Burnstock, G. (1998) · Neuroreport · doi:10.1097/00001756-199808030-00022 · Cited by 132
Properties of the pore‐forming P2X7 purinoceptor in mouse NTW8 microglial cells
Chessell, I. P., Michel, A. D., & Humphrey, P. P. A. (1997) · British Journal of Pharmacology · doi:10.1038/sj.bjp.0701278 · Cited by 126
Sources of adenosine released during neuromuscular transmission in the rat.
Smith, D. O. (1991) · The Journal of Physiology · doi:10.1113/jphysiol.1991.sp018388 · Cited by 118
Ionic effects on human recombinant P2X7 receptor function
Michel, A. D., Chessell, I. P., & Humphrey, P. P. A. (1999) · Naunyn-Schmiedeberg s Archives of Pharmacology · doi:10.1007/pl00005328 · Cited by 113
Jurányi, Z., Sperlágh, B., & Vizi, E. S. (1999) · Brain Research · doi:10.1016/s0006-8993(99)01169-5 · Cited by 99
Electron-immunocytochemical localization of P2X 1 receptors in the rat cerebellum
Loesch, A., & Burnstock, G. (1998) · Cell and Tissue Research · doi:10.1007/s004410051175 · Cited by 93
Petrou, S., Uğur, M., Drummond, R. M., Singer, J. J., & Walsh, J. V. (1997) · FEBS Letters · doi:10.1016/s0014-5793(97)00700-x · Cited by 92
Ion channel involvement in anoxic depolarization induced by cardiac arrest in rat brain
Xie, Y., Zacharias, E., Hoff, P., & Tegtmeier, F. (1995) · Journal of Cerebral Blood Flow & Metabolism · doi:10.1038/jcbfm.1995.72 · Cited by 91
ATP potentiates spontaneous transmitter release at developing neuromuscular synapses
Fu, W., & Poo, M. (1991) · Neuron · doi:10.1016/0896-6273(91)90179-4 · Cited by 77
Lutz, P. L., & Kabler, S. L. (1997) · Brain Research · doi:10.1016/s0006-8993(97)00719-1 · Cited by 77
Chapter 23 Purinergic regulation of acetylcholine release
Ribeiro, J. A., Cunha, R. A., Correia‐de‐Sá, P., & Sebastião, A. M. (1996) · Progress in brain research · doi:10.1016/s0079-6123(08)62107-x · Cited by 73
Khakh, B. S., Smith, W. B., Chiu, C., Ju, D., Davidson, N., & Lester, H. A. (2001) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.081089198 · Cited by 72
Li, G., Lee, E. M., Blair, D. H., Holding, C. A., Poronnik, P., Cook, D. I., Barden, J. A., & Bennett, M. R. (2000) · Journal of Biological Chemistry · doi:10.1074/jbc.m910277199 · Cited by 63
Properties of ATP receptor-mediated synaptic transmission in the rat medial habenula
Edwards, F. A., Robertson, S. J., & Gibb, A. J. (1997) · Neuropharmacology · doi:10.1016/s0028-3908(97)00127-5 · Cited by 60
P2X2 receptor immunoreactivity in the dorsal vagal complex and area postrema of the rat
Atkinson, L., Batten, T. F. C., & Deuchars, J. (2000) · Neuroscience · doi:10.1016/s0306-4522(00)00233-5 · Cited by 53
Salgado, A. I., Cunha, R. A., & Ribeiro, J. A. (2000) · Brain Research · doi:10.1016/s0006-8993(00)02679-2 · Cited by 50
Bardini, M., Lee, H. Y., & Burnstock, G. (1999) · Cell and Tissue Research · doi:10.1007/s004419900138 · Cited by 42
Bardini, M., Lee, H. Y., & Burnstock, G. (2000) · Cell and Tissue Research · doi:10.1007/s004410050010 · Cited by 41
Chapter 3 ATP Permeabilization of the Plasma Membrane
Steinberg, T. H., & Silverstein, S. C. (1989) · Methods in cell biology · doi:10.1016/s0091-679x(08)61601-5 · Cited by 29
Distribution of P2X receptors in the rat adrenal gland
Afework, M., & Burnstock, G. (1999) · Cell and Tissue Research · doi:10.1007/s004410050067 · Cited by 29
Distribution of P2X receptors in the rat adrenal gland
Afework, M., & Burnstock, G. (1999) · Cell and Tissue Research · doi:10.1007/s004419900103 · Cited by 26
Prejunctional Adenosine and ATP Receptorsa
Silinsky, E. M., Hunt, J. M., Solsona, C., & Hirsh, J. K. (1990) · Annals of the New York Academy of Sciences · doi:10.1111/j.1749-6632.1990.tb37683.x · Cited by 16
DISTRIBUTION OF P2X RECEPTORS IN THE URINARY BLADDER AND THE URETER OF THE RAT
LEE, H. Y., Bardini, M., & Burnstock, G. (2000) · The Journal of Urology · doi:10.1097/00005392-200006000-00110 · Cited by 13
Changes in extracellular [K+] and [Ca2+] induced by anoxia in neonatal rabbit medulla
Morris, M. E., & Trippenbach, T. (1993) · American Journal of Physiology-Regulatory, Integrative and Comparative Physiology · doi:10.1152/ajpregu.1993.264.4.r761 · Cited by 9
Cited by
Sorted by citations
Molecular Physiology of P2X Receptors
North, R. A. (2002) · Physiological Reviews · doi:10.1152/physrev.00015.2002 · Cited by 2,919
Physiology and Pathophysiology of Purinergic Neurotransmission
Burnstock, G. (2007) · Physiological Reviews · doi:10.1152/physrev.00043.2006 · Cited by 1,609
Cellular Distribution and Functions of P2 Receptor Subtypes in Different Systems
Burnstock, G., & Knight, G. E. (2004) · International review of cytology · doi:10.1016/s0074-7696(04)40002-3 · Cited by 796
P2X7 receptor inhibition improves recovery after spinal cord injury
Wang, X., Arcuino, G., Takano, T., Lin, J. H.-C., Peng, W. G., Wan, P., Li, P., Xu, Q., Liu, Q. S., Goldman, S. A., et al. (2004) · Nature Medicine · doi:10.1038/nm1082 · Cited by 505
Activation and Regulation of Purinergic P2X Receptor Channels
Coddou, C., Yan, Z., Obšil, T., Huidobro-Toro, J. P., & Stojilković, S. S. (2011) · Pharmacological Reviews · doi:10.1124/pr.110.003129 · Cited by 520
Metadata
- Authors
- Deuchars, S. A., Atkinson, L., Brooke, R. E., Musa, H., Milligan, C. J., Batten, T. F. C., Buckley, N. J., Parson, S. H., & Deuchars, J.
- Affiliations
- University of Leeds (GB)
- ORCID
- Venue
- The Journal of Neuroscience
- Publisher
- Society for Neuroscience
- Published
- 2001-09-15
- Volume
- 21
- Issue
- 18
- Pages
- 7143-7152
- ISSN
- 0270-6474 (ISSN-L)
- 1529-2401
- PMC
- PMC6762981
- OpenAlex
- W1620679902
- Cited by
- 287
- References
- 53
- Citations by year
- 2026: 4 · 2025: 2 · 2024: 4 · 2023: 11 · 2022: 4 · 2021: 8 · 2020: 8 · 2019: 4 · 2018: 5 · 2017: 5 · 2016: 9 · 2015: 10 · 2014: 10 · 2013: 13 · 2012: 11
- FWCI
- 9.84
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Journal of Neuroscience (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Wellcome Trust
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Physiology › Adenosine and Purinergic Signaling
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Health Sciences › Medicine › Physiology (medical) › Pain Mechanisms and Treatments
- Keywords
- P2X7 receptor, presynaptic terminals, synaptic transmission, medulla oblongata, spinal cord, nodose ganglion, neuromuscular junction, motor nerve terminals, BzATP, oxidized ATP, Brilliant Blue G, glutamate release, vesicle exocytosis
- MeSH terms
- Animals, Central Nervous System, Immunohistochemistry, Male, Medulla Oblongata, Mice, Inbred C57BL, Synaptic Transmission, Neuromuscular Junction, Neurons, Nodose Ganglion, RNA, Messenger, Spinal Cord, Rats, Wistar, In Situ Hybridization, Presynaptic Terminals, Peripheral Nervous System, Receptors, Purinergic P2, Neurotransmitter Agents, Patch-Clamp Techniques, Muscle, Skeletal, Glutamic Acid, Reverse Transcriptase Polymerase Chain Reaction, Mice, Rats, Receptors, Purinergic P2X7
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
