The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
A Noninvasive Genetic/Pharmacologic Strategy for Visualizing Cell Morphology and Clonal Relationships in the Mouse
Open accessAbstract
Analysis of cellular morphology is the most general approach to neuronal classification. With the increased use of genetically engineered mice, there is a growing need for methods that can selectively visualize the morphologies of specified subsets of neurons. This capability is needed both to define cell morphologic phenotypes and to mark cells in a noninvasive manner for lineage studies. To this end, we describe a bipartite genetic system based on a Cre-estrogen receptor (ER) fusion protein that irreversibly activates a plasma membrane-bound alkaline phosphatase reporter gene by site-specific recombination. Because the efficiency and timing of gene rearrangement is controlled pharmacologically, a sparse subset of labeled cells can be generated from the set of CreER-expressing cells at any time during development. Histochemical visualization of alkaline phosphatase activity reveals neuronal morphology with strong and uniform labeling of all processes.
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“, 2001) and were backcrossed five generations onto the C56BL/6 background before breeding to ROSA26CreER(Tam) (Badea et al., 2003).”
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Metadata
- Authors
- Badea, T. C., Wang, Y., & Nathans, J.
- Venue
- The Journal of Neuroscience
- Publisher
- Society for Neuroscience
- Published
- 2003-03-15
- Volume
- 23
- Issue
- 6
- Pages
- 2314-2322
- ISSN
- 0270-6474 (ISSN-L)
- 1529-2401
- OpenAlex
- W1538926863
- Cited by
- 262
- References
- 1
- FWCI
- 1.00
- Type
- journal article
- Language
- en
- Open access
- green
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- Badea, T. C., Wang, Y., & Nathans, J. (2003). A Noninvasive Genetic/Pharmacologic Strategy for Visualizing Cell Morphology and Clonal Relationships in the Mouse. The Journal of Neuroscience. https://doi.org/10.1523/jneurosci.23-06-02314.2003
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