Antibodies as valuable neuroscience research tools versus reagents of mass distraction
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Editor's Note: Toolboxes are a new, occasional feature in the Journal designed to briefly highlight a new method or a resource of general use in neuroscience or to critically analyze existing approaches or methods. For more information, see [http://www.jneurosci.org/misc/sifa_features.shtml][1].
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There is uncertainty around the usefulness of antibodies against GPCRs.
“However, there is uncertainty around the usefulness of antibodies against GPCRs (Michel et al., 2009”
From Self-reported symptom burden in postural orthostatic tachycardia syndrome (POTS): A narrative review of observational and interventional studies (2023) · cites this paper as Rhodes and Trimmer, 2006
The paper's Abstract says many antibodies fail basic tests of activity or specificity, supporting uncertainty about antibody reliability generally. The passages do not address antibodies against GPCRs specifically, so the GPCR-specific part is not established by this source. No page number is provided in our copy. (LLM, conf=0.82)
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Cited by
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Microglial physiology: unique stimuli, specialized responses
Ransohoff, R. M., & Perry, V. H. (2009) · Annual Review of Immunology · doi:10.1146/annurev.immunol.021908.132528 · Cited by 1,858 · see citing works
Olsen, R. W., & Sieghart, W. (2008) · Pharmacological Reviews · doi:10.1124/pr.108.00505 · Cited by 1,064 · see citing works
How reliable are G-protein-coupled receptor antibodies?
Michel, M. C., Wieland, T., & Tsujimoto, G. (2009) · Naunyn-Schmiedeberg's Archives of Pharmacology · doi:10.1007/s00210-009-0395-y · Cited by 306 · see citing works
Alkire, M. T., Asher, C. D., Franciscus, A. M., & Hahn, E. L. (2009) · Anesthesiology · doi:10.1097/aln.0b013e31819c461c · Cited by 105 · see citing works
Knoop, I., Picariello, F., Jenkinson, E., Gall, N., Chisari, C., & Moss-Morris, R. (2023) · Autonomic Neuroscience · doi:10.1016/j.autneu.2022.103052 · Cited by 21 · see citing works
“G protein-coupled receptors (GPCRs) comprise a large class of proteins that regulate many physiological functions such as vision, smell, taste, neurotransmission, cardiac output, and pain perception (83, 84).”
Influential
- Authors
- Rhodes, K. J., & Trimmer, J. S.
- Affiliations
- University of California, Davis (US)
- ORCID
- Trimmer, James S.
- Venue
- The Journal of Neuroscience
- Publisher
- Society for Neuroscience
- Published
- 2006-08-02
- Volume
- 26
- Issue
- 31
- Pages
- 8017-8020
- ISSN
- 0270-6474 (ISSN-L)
- 1529-2401
- PMC
- PMC6673789
- OpenAlex
- W2055540093
- Cited by
- 162
- References
- 0
- Citations by year
- 2026: 2 · 2025: 6 · 2024: 3 · 2023: 9 · 2022: 3 · 2021: 7 · 2020: 6 · 2019: 5 · 2018: 6 · 2017: 6 · 2016: 6 · 2015: 13 · 2014: 13 · 2013: 10 · 2012: 14
- FWCI
- 8.62
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Journal of Neuroscience (publishedVersion, cc-by-nc-sa)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institute of Mental Health
- National Institute of Neurological Disorders and Stroke (U24NS50606, U24NS050606)
- Topics
- Health Sciences › Medicine › Radiology, Nuclear Medicine and Imaging › Monoclonal and Polyclonal Antibodies Research
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Molecular Biology Techniques and Applications
- Keywords
- immunoglobulins, antibody validation, chemical reagents, scientific rigor
- MeSH terms
- Animals, Antibodies, Antigen-Antibody Reactions, Humans, Immunoassay, Immunohistochemistry, Indicators and Reagents, Neurosciences, Research Design, Protein Interaction Mapping
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