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Cited by 43Cellular transport and secretionRNA Research and SplicingRNA and protein synthesis mechanisms

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RNA localization and co‐translational interactions control RAB13 GTPase function and cell migration

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Moissoglu, Konstadinos, Stueland, Michael, Gasparski, Alexander N, Wang, Tianhong, Jenkins, Lisa M, Hastings, Michelle L, Mili, Stavroula·2020·The EMBO Journal
DOI 10.15252/embj.2020104958PMC PMC7604616

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Abstract

Numerous RNAs exhibit specific distribution patterns in mammalian cells. However, the functional and mechanistic consequences are relatively unknown. Here, we investigate the functional role of RNA localization at cellular protrusions of migrating mesenchymal cells, using as a model the RAB13 RNA, which encodes a GTPase important for vesicle‐mediated membrane trafficking. While RAB13 RNA is enriched at peripheral protrusions, the expressed protein is concentrated perinuclearly. By specifically preventing RAB13 RNA localization, we show that peripheral RAB13 translation is not important for the overall distribution of the RAB13 protein or its ability to associate with membranes, but is required for full activation of the GTPase and for efficient cell migration. RAB13 translation leads to a co‐translational association of nascent RAB13 with the exchange factor RABIF. Our results indicate that RAB13‐RABIF association at the periphery is required for directing RAB13 GTPase activity to promote cell migration. Thus, translation of RAB13 in specific subcellular environments imparts the protein with distinct properties and highlights a means of controlling protein function through local RNA translation.

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Metadata

Authors
Moissoglu, K., Stueland, M., Gasparski, A. N., Wang, T., Jenkins, L. M., Hastings, M. L., & Mili, S.
Affiliations
  • National Institutes of Health (US)
  • Rosalind Franklin University of Medicine and Science (US)
Corresponding author
Mili, Stavroula
ORCID
Venue
The EMBO Journal
Publisher
Springer Science and Business Media LLC
Published
2020-09-18
Volume
39
Issue
21
Pages
e104958-e104958
ISSN
  • 0261-4189 (ISSN-L)
  • 1460-2075
DOI
10.15252/embj.2020104958
PMC
PMC7604616
OpenAlex
W3087518429
Cited by
43
References
2
Citations by year
2026: 3 · 2025: 5 · 2024: 5 · 2023: 14 · 2022: 6 · 2021: 7 · 2020: 3
FWCI
3.74
Type
journal article
Language
en
Open access
Licence
Locations
  • The EMBO Journal (publishedVersion, public-domain)
  • bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, public-domain)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
Funders
HHS | NIH | National Cancer Institute (1ZIA BC011501)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Research and Splicing
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA and protein synthesis mechanisms
Keywords
Rab13, cell migration, GTPase activation, local translation, cell protrusions, mesenchymal cells, membrane trafficking, vesicle trafficking, subcellular protein distribution
MeSH terms
Animals, Cell Movement, Humans, Mesoderm, RNA, GTP Phosphohydrolases, Guanine Nucleotide Exchange Factors, rab GTP-Binding Proteins, Protein Transport, Cell Surface Extensions, NIH 3T3 Cells, Mice, HEK293 Cells

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