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Cited by 89Plant Pathogenic Bacteria StudiesPlant-Microbe Interactions and ImmunityAutophagy in Disease and Therapy

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A bacterial effector counteracts host autophagy by promoting degradation of an autophagy component

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Leong, Jia Xuan, Raffeiner, Margot, Spinti, Daniela, Langin, Gautier, Franz‐Wachtel, Mirita, Guzman, Andrew R, Kim, Jung‐Gun, Pandey, Pooja, Minina, Alyona E, Macek, Boris, Hafrén, Anders, Bozkurt, Tolga O, Mudgett, Mary Beth, Börnke, Frederik, Hofius, Daniel, Üstün, Suayib·2022·The EMBO Journal
DOI 10.15252/embj.2021110352PMC PMC9251887

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Abstract

Beyond its role in cellular homeostasis, autophagy plays anti- and promicrobial roles in host-microbe interactions, both in animals and plants. One prominent role of antimicrobial autophagy is to degrade intracellular pathogens or microbial molecules, in a process termed xenophagy. Consequently, microbes evolved mechanisms to hijack or modulate autophagy to escape elimination. Although well-described in animals, the extent to which xenophagy contributes to plant-bacteria interactions remains unknown. Here, we provide evidence that Xanthomonas campestris pv. vesicatoria (Xcv) suppresses host autophagy by utilizing type-III effector XopL. XopL interacts with and degrades the autophagy component SH3P2 via its E3 ligase activity to promote infection. Intriguingly, XopL is targeted for degradation by defense-related selective autophagy mediated by NBR1/Joka2, revealing a complex antagonistic interplay between XopL and the host autophagy machinery. Our results implicate plant antimicrobial autophagy in the depletion of a bacterial virulence factor and unravel an unprecedented pathogen strategy to counteract defense-related autophagy in plant-bacteria interactions.

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Metadata

Authors
Leong, J. X., Raffeiner, M., Spinti, D., Langin, G., Franz‐Wachtel, M., Guzman, A. R., Kim, J., Pandey, P., Minina, A. E., Macek, B., Hafrén, A., Bozkurt, T. O., Mudgett, M. B., Börnke, F., Hofius, D., & Üstün, S.
Affiliations
  • University of Tübingen (DE)
  • Leibniz Institute of Vegetable and Ornamental Crops (DE)
  • Stanford University (US)
  • Imperial College London (GB)
  • Swedish University of Agricultural Sciences (SE)
  • University of Potsdam (DE)
ORCID
Venue
The EMBO Journal
Publisher
Springer Science and Business Media LLC
Published
2022-05-27
Volume
41
Issue
13
Pages
e110352-e110352
ISSN
  • 0261-4189 (ISSN-L)
  • 1460-2075
DOI
10.15252/embj.2021110352
PMC
PMC9251887
OpenAlex
W4281979571
Cited by
89
References
3
Citations by year
2026: 15 · 2025: 17 · 2024: 19 · 2023: 25 · 2022: 10
FWCI
9.78
Type
journal article
Language
en
Open access
Licence
Locations
  • The EMBO Journal (publishedVersion, cc-by)
  • bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by-nc)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • Epsilon Open Archive (Sveriges lantbruksuniversitet biblioteket (Swedish University of Agricultural Sciences)) (acceptedVersion)
  • publish.UP (University of Potsdam) (submittedVersion, cc-by)
Funders
  • Deutsche Forschungsgemeinschaft (GZ, SFB1101, BO1961/5‐2, UE188/2‐1)
  • EC | Horizon Europe | Excellent Science | HORIZON EUROPE Marie Sklodowska‐Curie Actions (799433)
  • Svenska Forskningsrådet Formas (2017‐01596, 2016‐01044)
  • Vetenskapsrådet (2016‐04562, 2020‐05327)
  • UKRI | Biotechnology and Biological Sciences Research Council (BB/T006102/1)
  • NSF | BIO | Division of Integrative Organismal Systems (2026368)
Topics
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Pathogenic Bacteria Studies
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant-Microbe Interactions and Immunity
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Autophagy in Disease and Therapy
Keywords
autophagy, xenophagy, plant-bacteria interactions, Xanthomonas campestris pv. vesicatoria, type III effectors, E3 ubiquitin ligase, NBR1, selective autophagy, plant defense
MeSH terms
Animals, Autophagy, Bacteria, Plant Diseases, Virulence Factors, Host-Pathogen Interactions

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