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Cited by 98Cellular transport and secretionLipid metabolism and biosynthesisProtein Kinase Regulation and GTPase Signaling

Plasticity of PI4KIIIα interactions at the plasma membrane

Open access
Chung, Jeeyun, Nakatsu, Fubito, Baskin, Jeremy M, De Camilli, Pietro·2015·The EMBO Reports
DOI 10.15252/embr.201439151PMC PMC4364870
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Abstract

Plasma membrane PI4P is an important direct regulator of many processes that occur at the plasma membrane and also a biosynthetic precursor of PI(4,5)P2 and its downstream metabolites. The majority of this PI4P pool is synthesized by an evolutionarily conserved complex, which has as its core the PI 4-kinase PI4KIIIα (Stt4 in yeast) and also comprises TTC7 (Ypp1 in yeast) and the peripheral plasma membrane protein EFR3. While EFR3 has been implicated in the recruitment of PI4KIIIα via TTC7, the plasma membrane protein Sfk1 was also shown to participate in this targeting and activity in yeast. Here, we identify a member of the TMEM150 family as a functional homologue of Sfk1 in mammalian cells and demonstrate a role for this protein in the homeostatic regulation of PI(4,5)P2 at the plasma membrane. We also show that the presence of TMEM150A strongly reduces the association of TTC7 with the EFR3-PI4KIIIα complex, without impairing the localization of PI4KIIIα at the plasma membrane. Collectively our results suggest a plasticity of the molecular interactions that control PI4KIIIα localization and function.

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Cited by

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Authors
Chung, J., Nakatsu, F., Baskin, J. M., & De Camilli, P.
Affiliations
Howard Hughes Medical Institute (US)
ORCID
Venue
The EMBO Reports
Publisher
Springer Science and Business Media LLC
Published
2015-01-21
Volume
16
Issue
3
Pages
312-320
ISSN
  • 1469-221X (ISSN-L)
  • 1469-3178
DOI
10.15252/embr.201439151
PMC
PMC4364870
OpenAlex
W2128726985
Cited by
98
References
1
Citations by year
2026: 5 · 2025: 9 · 2024: 4 · 2023: 7 · 2022: 13 · 2021: 11 · 2020: 20 · 2019: 4 · 2018: 11 · 2017: 3 · 2016: 8 · 2015: 3
FWCI
4.62
Type
journal article
Language
en
Open access
Licence
Locations
  • EMBO Reports (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • NIH (R37NS036251, DK082700, DA018343, T32GM007223, K99GM110121)
  • Simons Foundation
  • Jane Coffin Childs Fund
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Kinase Regulation and GTPase Signaling
Keywords
PI4KIIIα, phosphatidylinositol 4-phosphate, protein-protein interaction, phosphoinositide metabolism
MeSH terms
Cell Membrane, HeLa Cells, Homeostasis, Humans, Membrane Proteins, Peptide Termination Factors, Proteins, Immunoblotting, Minor Histocompatibility Antigens, Phosphotransferases (Alcohol Group Acceptor), Multiprotein Complexes, Immunoprecipitation

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