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Recognition of centromere‐specific histone Cse4 by the inner kinetochore Okp1‐Ame1 complex
Open accessPublished version of a preprint
Abstract
Successful mitosis depends on the timely establishment of correct chromosomal attachments to microtubules. The kinetochore, a modular multiprotein complex, mediates this connection by recognizing specialized chromatin containing a histone H3 variant called Cse4 in budding yeast and CENP‐A in vertebrates. Structural features of the kinetochore that enable discrimination between Cse4/CENP‐A and H3 have been identified in several species. How and when these contribute to centromere recognition and how they relate to the overall structure of the inner kinetochore are unsettled questions. More generally, this molecular recognition ensures that only one kinetochore is built on each chromatid and that this happens at the right place on the chromatin fiber. We have determined the crystal structure of a Cse4 peptide bound to the essential inner kinetochore Okp1‐Ame1 heterodimer from budding yeast. The structure and related experiments show in detail an essential point of Cse4 contact and provide information about the arrangement of the inner kinetochore.
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Highly accurate protein structure prediction with AlphaFold
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Metadata
- Authors
- Deng, S., Cai, J., Harrison, S. C., Zhou, H., & Hinshaw, S. M.
- Affiliations
- Howard Hughes Medical Institute (US)
- University of California San Diego (US)
- Stanford Medicine (US)
- Corresponding author
- Hinshaw, Stephen M
- Venue
- The EMBO Reports
- Published
- 2023-11-20
- Volume
- 24
- Issue
- 12
- Pages
- e57702-e57702
- ISSN
- 1469-221X (ISSN-L)
- 1469-3178
- PMC
- PMC10702835
- OpenAlex
- W4388852205
- Cited by
- 10
- References
- 1
- Citations by year
- 2026: 4 · 2025: 2 · 2024: 2 · 2023: 2
- FWCI
- 1.44
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Locations
- EMBO Reports (publishedVersion, cc-by)
- bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- eScholarship (California Digital Library) (submittedVersion)
- OSTI OAI (U.S. Department of Energy Office of Scientific and Technical Information) (submittedVersion)
- Funders
- DOE | NNSA | LDRD | Argonne National Laboratory (DE‐AC02‐06CH11357)
- HHS | NIH | National Institute of General Medical Sciences (GM124165, GM116897)
- Howard Hughes Medical Institute
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Microtubule and mitosis dynamics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Nuclear Structure and Function
- Keywords
- Cse4, inner kinetochore, histone H3 variant, CENH3, kinetochore-microtubule attachment, mitosis, Saccharomyces cerevisiae, crystal structure, kinetochore assembly
- MeSH terms
- Centromere Protein A, Centromere, Chromatin, Chromosomal Proteins, Non-Histone, DNA-Binding Proteins, Saccharomycetales, Histones, Microtubule-Associated Proteins, Kinetochores, Cell Cycle Proteins, Saccharomyces cerevisiae Proteins
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