Roles of Spheroid Formation of Hepatocytes in Liver Tissue Engineering
Open accessAbstract
The liver plays an important role in a broad spectrum of physiological functions and detoxifies endogenous and exogenous substances. The liver failure is associated with a high risk of mortality because it is one of important organs in our body. Various bioartificial liver (BAL) systems have been used for clinical trials as a bridge for liver transplantations in patients with liver failure. Long term and stable liver-specific functions of hepatocytes in the development of BAL support systems should be considered. Spheroid formation of hepatocytes enhances liver-specific functions. In this review, hepatocyte spheroid formation methods such as galactose density, topology of extracellular matrix, micro-molding technique, hanging-drop culture, non-adhesive surface, positive charged surface, spinner culture, rocked technique, medium component, external forces, coculture system and polymeric nanospheres are explained for enhancing liver-specific functions.
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Spheroid cell culture has been developed for research on cancer, stem cells, and many organs, including liver, kidney, and heart.
“This approach has been developed in the research of cancer (7, 76, 102), stem cells (17, 120), and many organs (such as liver, kidney, heart, etc.) (3,16,64,75).”
From Modeling physiological events in 2D vs. 3D cell culture (2017) · cites this paper as [64]
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- Authors
- Jiang, H.-L., Kim, Y.-K., Cho, K.-H., Jang, Y.-C., Choi, Y.-J., Chung, J.-H., & Cho, C.-S.
- Affiliations
- Seoul National University (KR)
- Hallym University (KR)
- Venue
- International Journal of Stem Cells
- Published
- 2010-05-31
- Volume
- 3
- Issue
- 2
- Pages
- 69-73
- ISSN
- 2005-3606 (ISSN-L)
- 2005-5447
- PMC
- PMC4021799
- OpenAlex
- W1970117644
- Cited by
- 22
- References
- 42
- Citations by year
- 2026: 1 · 2025: 1 · 2024: 2 · 2023: 2 · 2021: 2 · 2020: 1 · 2019: 2 · 2018: 4 · 2017: 2 · 2016: 2 · 2015: 3
- FWCI
- 0.00
- Type
- journal article
- Language
- en
- Open access
- green
- europepmc.org
- Locations
- International Journal of Stem Cells (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
- Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
- Health Sciences › Medicine › Hepatology › Liver physiology and pathology
- Keywords
- hepatocyte spheroids, liver tissue engineering, bioartificial liver, liver failure, liver transplantation, liver-specific function, hanging drop culture, spinner culture, micromolding, co-culture, polymeric nanospheres, external forces
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