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Theoretical Evaluation of Voltage Inducement on Internal Membranes of Biological Cells Exposed to Electric Fields
Open accessAbstract
Several reports have recently been published on effects of very short and intense electric pulses on cellular organelles; in a number of cases, the cell plasma membrane appeared to be affected less than certain organelle membranes, whereas with longer and less intense pulses the opposite is the case. The effects are the consequence of the voltages induced on the membranes, and in this article we investigate the conditions under which the induced voltage on an organelle membrane could exceed its counterpart on the cell membrane. This would provide a possible explanation of the observed effects of very short pulses. Frequency-domain analysis yields an insight into the dependence of the voltage inducement on the electric and geometric parameters characterizing the cell and its vicinity. We show that at sufficiently high field frequencies, for a range of parameter values the voltage induced on the organelle membrane can indeed exceed the voltage induced on the cell membrane. Particularly, this can occur if the organelle interior is electrically more conductive than the cytosol, or if the organelle membrane has a lower dielectric permittivity than the cell membrane, and we discuss the plausibility of these conditions. Time-domain analysis is then used to determine the courses of the voltage induced on the membranes by pulses with risetimes and durations in the nanosecond range. The particularly high resting voltage in mitochondria, to which the induced voltage superimposes, could contribute to the explanation why these organelles are the primary target of many observed effects.
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“as the frequency of the field increases, the high membrane field gain diminishes, allowing for higher field intensities to penetrate into the cell.”
From An Overview of Sub-Cellular Mechanisms Involved in the Action of TTFields (2016) · cites this paper as [31]
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“As a result, with further shortening of the pulses from the submicrosecond to the nanosecond range, the amplitude of the TMV induced on the plasma membrane is increasingly attenuated (96, 98) and gradually becomes comparable to the TMV induced on organelle membranes in the cell interior (97).”
BackgroundResultModeling Electroporation in a Single Cell
Krassowska, W., & Filev, P. D. (2007) · Biophysical Journal · doi:10.1529/biophysj.106.094235 · Cited by 501
Cell membrane electroporation- Part 1: The phenomenon
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“To determine ΔΨ m induced by shorter pulses or higher frequencies, the permittivities of the electrolytes surrounding the membrane also have to be taken into account, leading to further generalization of (5) to a second-order model [35]–[38].”
BackgroundIuliis, G. N. D., Newey, R. J., King, B. V., & Aitken, R. J. (2009) · PLoS ONE · doi:10.1371/journal.pone.0006446 · Cited by 452
Metadata
- Authors
- Kotnik, T., & Miklavčič, D.
- Affiliations
- University of Ljubljana (SI)
- Corresponding author
- Kotnik, Tadej
- ORCID
- Kotnik, Tadej
- Venue
- Biophysical Journal
- Publisher
- Elsevier BV
- Published
- 2006-01
- Volume
- 90
- Issue
- 2
- Pages
- 480-491
- ISSN
- 0006-3495 (ISSN-L)
- PMC
- PMC1367054
- OpenAlex
- W2106955112
- Cited by
- 318
- References
- 68
- Citations by year
- 2026: 13 · 2025: 11 · 2024: 15 · 2023: 22 · 2022: 12 · 2021: 12 · 2020: 17 · 2019: 20 · 2018: 19 · 2017: 26 · 2016: 19 · 2015: 12 · 2014: 19 · 2013: 17 · 2012: 17
- FWCI
- 4.26
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Biophysical Journal (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biophysics › Electromagnetic Fields and Biological Effects
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biotechnology › Microbial Inactivation Methods
- Physical Sciences › Engineering › Electrical and Electronic Engineering › Electrical and Electromagnetic Research
- Keywords
- electric field effects on cells, organelle membranes, nanosecond pulses, frequency domain analysis, time-domain analysis, membrane conductivity
- MeSH terms
- Animals, Biological Transport, Active, Biophysics, Cell Membrane, Cell Membrane Permeability, Cytosol, Electric Conductivity, Electromagnetic Fields, Intracellular Membranes, Membrane Fluidity, Membrane Lipids, Membrane Potentials, Models, Theoretical, Time Factors
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