The DOI matches its Crossref record; 67 of the 68 references it cites were found in a registry or an index.
Theoretical Evaluation of Voltage Inducement on Internal Membranes of Biological Cells Exposed to Electric Fields
Open accessAbstract
Several reports have recently been published on effects of very short and intense electric pulses on cellular organelles; in a number of cases, the cell plasma membrane appeared to be affected less than certain organelle membranes, whereas with longer and less intense pulses the opposite is the case. The effects are the consequence of the voltages induced on the membranes, and in this article we investigate the conditions under which the induced voltage on an organelle membrane could exceed its counterpart on the cell membrane. This would provide a possible explanation of the observed effects of very short pulses. Frequency-domain analysis yields an insight into the dependence of the voltage inducement on the electric and geometric parameters characterizing the cell and its vicinity. We show that at sufficiently high field frequencies, for a range of parameter values the voltage induced on the organelle membrane can indeed exceed the voltage induced on the cell membrane. Particularly, this can occur if the organelle interior is electrically more conductive than the cytosol, or if the organelle membrane has a lower dielectric permittivity than the cell membrane, and we discuss the plausibility of these conditions. Time-domain analysis is then used to determine the courses of the voltage induced on the membranes by pulses with risetimes and durations in the nanosecond range. The particularly high resting voltage in mitochondria, to which the induced voltage superimposes, could contribute to the explanation why these organelles are the primary target of many observed effects.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
In the citing paper
“as the frequency of the field increases, the high membrane field gain diminishes, allowing for higher field intensities to penetrate into the cell.”
From An Overview of Sub-Cellular Mechanisms Involved in the Action of TTFields (2016) · cites this paper as [31]
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Methods of Theoretical Physics
Morse, P. M., Feshbach, H., & Hill, E. L. (1954) · American Journal of Physics · doi:10.1119/1.1933765 · Cited by 13,197
Lodish, H. F. (1986) · Cited by 5,650
Methods of theoretical physics
Domenicali, C. A. (1954) · Journal of the Franklin Institute · doi:10.1016/0016-0032(54)90634-1 · Cited by 4,893
Mathematical Methods for Physicists
Arfken, G. B., & Mathews, J. (1972) · American Journal of Physics · doi:10.1119/1.1988084 · Cited by 4,069
Fröhlich, H., & Maradudin, A. A. (1949) · CERN Document Server (European Organization for Nuclear Research) · Cited by 2,213
Gautier, J. (1993) · Trends in Biochemical Sciences · doi:10.1016/0968-0004(93)90025-i · Cited by 2,200
Theory of electroporation: A review
Weaver, J. C., & Chizmadzhev, Y. A. (1996) · Bioelectrochemistry and Bioenergetics · doi:10.1016/s0302-4598(96)05062-3 · Cited by 1,639
Mathematical Methods for Physicists
(1995) · Elsevier eBooks · doi:10.1016/c2009-1-28537-6 · Cited by 1,255
Electroporation and Electrofusion in Cell Biology
Neumann, E., Sowers, A. E., & Jordan, C. A. (1989) · Springer eBooks · doi:10.1007/978-1-4899-2528-2 · Cited by 947
Oberhettinger, F., & Badii, L. (1973) · Springer eBooks · doi:10.1007/978-3-642-65645-3 · Cited by 762
Cited by
Sorted by citations
Electroporation-based technologies for medicine: principles, applications, and challenges.
Yarmush, M., Golberg, A., Serša, G., Kotnik, T., & Miklavčič, D. (2014) · Annual Review of Biomedical Engineering · doi:10.1146/annurev-bioeng-071813-104622 · Cited by 813
Membrane Electroporation and Electropermeabilization: Mechanisms and Models
Kotnik, T., Rems, L., Tarek, M., & Miklavčič, D. (2019) · Annual Review of Biophysics · doi:10.1146/annurev-biophys-052118-115451 · Cited by 813
“As a result, with further shortening of the pulses from the submicrosecond to the nanosecond range, the amplitude of the TMV induced on the plasma membrane is increasingly attenuated (96, 98) and gradually becomes comparable to the TMV induced on organelle membranes in the cell interior (97).”
BackgroundResultModeling Electroporation in a Single Cell
Krassowska, W., & Filev, P. D. (2007) · Biophysical Journal · doi:10.1529/biophysj.106.094235 · Cited by 501
Cell membrane electroporation- Part 1: The phenomenon
Kotnik, T., Kramar, P., Pucihar, G., Damijan Miklavčič, A., & Tarek, M. (2012) · IEEE Electrical Insulation Magazine · doi:10.1109/mei.2012.6268438 · Cited by 479
“To determine ΔΨ m induced by shorter pulses or higher frequencies, the permittivities of the electrolytes surrounding the membrane also have to be taken into account, leading to further generalization of (5) to a second-order model [35]–[38].”
BackgroundIuliis, G. N. D., Newey, R. J., King, B. V., & Aitken, R. J. (2009) · PLoS ONE · doi:10.1371/journal.pone.0006446 · Cited by 452
Geboers, B., Scheffer, H. J., Graybill, P. M., Ruarus, A. H., Nieuwenhuizen, S., Puijk, R. S., van den Tol, M. P., Davalos, R. V., Rubinsky, B., de Gruijl, T. D., et al. (2020) · Radiology · doi:10.1148/radiol.2020192190 · Cited by 402
Thrivikraman, G., Boda, S. K., & Basu, B. (2017) · Biomaterials · doi:10.1016/j.biomaterials.2017.10.003 · Cited by 386
“Notably, since the cells are electrically shielded by the cell membrane, the extent of shielding is frequency dependent in an alternating field and depends on the rise time and pulse duration [10].”
BackgroundArena, C. B., Sano, M. B., Rossmeisl, J. H., Caldwell, J. L., Garcia, P. A., Rylander, M. N., & Davalos, R. V. (2011) · BioMedical Engineering OnLine · doi:10.1186/1475-925x-10-102 · Cited by 383
“Kotnik T, Miklavcic D: Theoretical evaluation of voltage inducement on internal membranes of biological cells exposed to electric fields.”
BackgroundInfluentialPakhomov, A. G., Kolb, J. F., White, J. A., Joshi, R. P., Xiao, S. H., & Schoenbach, K. H. (2007) · Bioelectromagnetics · doi:10.1002/bem.20354 · Cited by 294
“At the same time, analytical models of nsPEF effect on cells [Foster, 2000; Joshi et al., 2002; Gowrishankar and Weaver, 2006; Kotnik and Miklavcic, 2006; Smyth et al., 2006] and numerical simulations [Tieleman, 2004; Hu et al., 2005; Tarek, 2005] suggested that the cell plasma membrane is not…”
MethodThe application of PEF technology in food processing and human nutrition
Nowosad, K., Sujka, M., Pankiewicz, U., & Kowalski, R. (2020) · Journal of food science and technology · doi:10.1007/s13197-020-04512-4 · Cited by 173
Induced Transmembrane Voltage and Its Correlation with Electroporation-Mediated Molecular Transport
Kotnik, T., Pucihar, G., & Damijan Miklavčič, A. (2010) · The Journal of Membrane Biology · doi:10.1007/s00232-010-9279-9 · Cited by 236
“For even higher field frequencies and even shorter pulses, the second-order Schwan equation, in which the dielectric permittivities of the cytoplasm and the extracellular medium are also taken into account, must be used (Grosse and Schwan 1992; Kotnik et al. 1998; Kotnik and Miklavčič 2000b, 2006).”
MethodAdvanced Electroporation Techniques in Biology and Medicine
Pakhomov, A. G., Damijan Miklavčič, A., & Markov, M. S. (2010) · CRC Press eBooks · doi:10.1201/ebk1439819067 · Cited by 234
Membrane permeabilization and cell damage by ultrashort electric field shocks
Pakhomov, A. G., Shevin, R. S., White, J. A., Kolb, J. F., Pakhomova, O. N., Joshi, R. P., & Schoenbach, K. H. (2007) · Archives of Biochemistry and Biophysics · doi:10.1016/j.abb.2007.05.003 · Cited by 197
“However, analytical and numerical models of nsPEF interaction with cells suggested that pores should form in cell plasma membrane as well, not just in organelles [12,13,27].”
BackgroundResultNapotnik, T. B., Reberšek, M., Vernier, P. T., Mali, B. Č., & Damijan Miklavčič, A. (2016) · Bioelectrochemistry · doi:10.1016/j.bioelechem.2016.02.011 · Cited by 195
“With the use of nanosecond pulses, as pulses shorten, more intracellular effects and less effects on plasma membrane can be expected [15,19,29,30].”
BackgroundAnalysis of Plasma Membrane Integrity by Fluorescent Detection of Tl+ Uptake
Bowman, A. M., Nesin, O. M., Pakhomova, O. N., & Pakhomov, A. G. (2010) · The Journal of Membrane Biology · doi:10.1007/s00232-010-9269-y · Cited by 192
“The possibility of electroporation of organelles by USEPs was generally supported by theoretical models (Gowrishankar and Weaver 2006; Hu et al. 2005; Kotnik and Miklavcic 2006; Smith and Weaver 2008); however, it was also suggested that the plasma membrane should not be ‘‘exempt’’ from poration.”
Aycock, K. N., & Davalos, R. V. (2019) · Bioelectricity · doi:10.1089/bioe.2019.0029 · Cited by 189
Molecular Dynamics Simulations of Lipid Membrane Electroporation
Delemotte, L., & Tarek, M. (2012) · The Journal of Membrane Biology · doi:10.1007/s00232-012-9434-6 · Cited by 188
“…based on the Laplace equation (see, e.g., Pauly and Schwan 1959 for the first-order analysis on a spherical vesicle; Kotnik et al. 1998 for the second-order analysis; and Kotnik and Miklavcic 2006 for the second-order analysis for two concentric spherical vesicles, i.e., modeling an organelle).”
MethodTutorial: Electroporation of cells in complex materials and tissue
Rems, L., & Miklavčič, D. (2016) · Journal of Applied Physics · doi:10.1063/1.4949264 · Cited by 189
Kinetics of Transmembrane Transport of Small Molecules into Electropermeabilized Cells
Pucihar, G., Kotnik, T., Damijan Miklavčič, A., & Teissié, J. (2008) · Biophysical Journal · doi:10.1529/biophysj.108.135541 · Cited by 179
Pucihar, G., Damijan Miklavčič, A., & Kotnik, T. (2009) · IEEE Transactions on Biomedical Engineering · doi:10.1109/tbme.2009.2014244 · Cited by 176
“where a, fS1 , fS2 , τ1, and τ2 are given in [27] and [36].”
BackgroundNanosecond electric pulses cause mitochondrial membrane permeabilization in Jurkat cells
Napotnik, T. B., Wu, Y., Gundersen, M. A., Damijan Miklavčič, A., & Vernier, P. T. (2011) · Bioelectromagnetics · doi:10.1002/bem.20707 · Cited by 171
“Theoretical studies predict that mitochondria could be the target of nsEP electroporation [Kotnik and Miklavcic, 2006], and it has been shown that nsEP exposure causes permeabilization of intracellular organelles such as intracellular granules of granulocytes [Schoenbach et al., 2001], large…”
BackgroundInfluentialInvestigating ROS sources in male infertility: A common end for numerous pathways
Lavranos, G. M., Balla, M., Tzortzopoulou, A., Syriou, V., & Angelopoulou, R. (2012) · Reproductive Toxicology · doi:10.1016/j.reprotox.2012.06.007 · Cited by 166
Primary pathways of intracellular Ca2+ mobilization by nanosecond pulsed electric field
Semenov, I., Xiao, S. H., & Pakhomov, A. G. (2012) · Biochimica et Biophysica Acta (BBA) - Biomembranes · doi:10.1016/j.bbamem.2012.11.032 · Cited by 166
“These channels are activated by membrane depolarization by 30–50 mV, whereas the transmembrane potential required for electroporation is about an order of magnitude greater [1,2,8].”
A review of sublethal effects of pulsed electric field on cells in food processing
Wang, M., Wang, L., Bekhit, A. E. A., Yang, J. Y., Hou, Z., Wang, Y.-Z., Dai, Q., & Zeng, X. (2017) · Journal of Food Engineering · doi:10.1016/j.jfoodeng.2017.11.035 · Cited by 129
Wenger, C., Miranda, P. C., Salvador, R., Thielscher, A., Bomzon, Z., Giladi, M., Mrugala, M. M., & Korshoej, A. R. (2018) · IEEE Reviews in Biomedical Engineering · doi:10.1109/rbme.2017.2765282 · Cited by 129
Theoretical Considerations of Tissue Electroporation With High-Frequency Bipolar Pulses
Arena, C. B., Sano, M. B., Rylander, M. N., & Davalos, R. V. (2010) · IEEE Transactions on Biomedical Engineering · doi:10.1109/tbme.2010.2102021 · Cited by 128
“Experimentally, bipolar pulses reduce electrolytic contamination [27] and the required field strength for reversible electroporation, while the field strength required for IRE remains unchanged [26].”
BackgroundSano, M. B., Arena, C. B., DeWitt, M. R., Saur, D., & Davalos, R. V. (2014) · Bioelectrochemistry · doi:10.1016/j.bioelechem.2014.07.010 · Cited by 125
“However, other dielectric properties of the nucleus may affect the membrane charging time [35,61].”
BackgroundMethodRen, W., & Beebe, S. J. (2011) · APOPTOSIS · doi:10.1007/s10495-010-0572-y · Cited by 122
“Nevertheless, modeling evidence suggests that under certain circumstances it is possible to have effects on intracellular membranes without effects on the plasma membrane [18].”
BackgroundHaandbæk, N., Bürgel, S. C., Heer, F., & Hierlemann, A. R. (2013) · Lab on a Chip · doi:10.1039/c3lc50866h · Cited by 116
Recruitment of the intracellular Ca2+ by ultrashort electric stimuli: The impact of pulse duration
Semenov, I., Xiao, S. H., Pakhomova, O. N., & Pakhomov, A. G. (2013) · Cell Calcium · doi:10.1016/j.ceca.2013.05.008 · Cited by 116
“The formation of small membrane pores (“nanopores”), concurrently in both PM and intracellular membranes was supported by advanced numerical models [15, 16].”
Ibey, B. L., Cantu, J. C., Pakhomova, O. N., Roth, C. C., Semenov, I., Beier, H. T., Tarango, M., Xiao, S. H., Schoenbach, K. H., & Pakhomov, A. G. (2013) · Biochemical and Biophysical Research Communications · doi:10.1016/j.bbrc.2013.12.004 · Cited by 112
“While the high-frequency components present with short-duration pulses are believed to be less effective at PM permeabilization they are hypothesized to cause intracellular membrane permeabilization [14, 21, 22].”
Ye, H., & Steiger, A. (2015) · Journal of NeuroEngineering and Rehabilitation · doi:10.1186/s12984-015-0061-1 · Cited by 107
“Kotnik T, Miklavcic D. Theoretical evaluation of voltage inducement on internal membranes of biological cells exposed to electric fields.”
BackgroundInfluentialRecent Advances in Microscale Electroporation
Choi, S., Khoo, H., & Hur, S. C. (2022) · Chemical Reviews · doi:10.1021/acs.chemrev.1c00677 · Cited by 109
Modulation of cell function by electric field: a high-resolution analysis
Taghian, T., Narmoneva, D. A., & Kogan, A. B. (2015) · Journal of The Royal Society Interface · doi:10.1098/rsif.2015.0153 · Cited by 105
“Several studies have modelled the cell as multiple concentric shells to determine the induced EF in the internal membranes [55,56].”
BackgroundCell membrane electroporation-Part 3: the equipment
Reberšek, M., Damijan Miklavčič, A., Bertacchini, C., & Sack, M. (2014) · IEEE Electrical Insulation Magazine · doi:10.1109/mei.2014.6804737 · Cited by 102
“An electroporator generates short HV pulses of specific shape, amplitude, duration, number, and repetition frequency [4], and the pulses are applied to the target cells or load through the electrodes [5].”
BackgroundBeebe, S. J., Chen, Y.-J., Sain, N. M., Schoenbach, K. H., & Xiao, S. H. (2012) · PLoS ONE · doi:10.1371/journal.pone.0051349 · Cited by 100
“outer membranes [9], possibly making poration pervasive across whole cells.”
Basic Features of a Cell Electroporation Model: Illustrative Behavior for Two Very Different Pulses
Son, R. S., Smith, K. C., Gowrishankar, T. R., Vernier, P. T., & Weaver, J. C. (2014) · The Journal of Membrane Biology · doi:10.1007/s00232-014-9699-z · Cited by 99
“Close examination reveals a much thinner green band near the membrane around the anodic pole, with two small green areas away from the anodic pole— these areas are transition regions that favor large pores (DeBruin and Krassowska 1999a; Krassowska and Filev 2007; Smith 2011).”
BackgroundMethodInfluentialTargeted cellular ablation based on the morphology of malignant cells
Ivey, J. W., Latouche, E. L., Sano, M. B., Rossmeisl, J. H., Davalos, R. V., & Verbridge, S. S. (2015) · Scientific Reports · doi:10.1038/srep17157 · Cited by 103
An Overview of Sub-Cellular Mechanisms Involved in the Action of TTFields
Tuszynski, J., Wenger, C., Friesen, D., & Preto, J. (2016) · International Journal of Environmental Research and Public Health · doi:10.3390/ijerph13111128 · Cited by 95
Effect of electromagnetic waves on human reproduction
Wdowiak, A., Mazurek, P. A., Wdowiak, A., & Bojar, I. (2017) · Annals of Agricultural and Environmental Medicine · doi:10.5604/12321966.1228394 · Cited by 95
A scaling law for membrane permeabilization with nanopulses
Schoenbach, K. H., Joshi, R. P., Beebe, S. J., & Baum, C. E. (2009) · IEEE Transactions on Dielectrics and Electrical Insulation · doi:10.1109/tdei.2009.5293932 · Cited by 95
“Modeling results using molecular dynamics [26-28] and continuum models [29-32] have provided insight into the mechanism of permeabilization of membranes with a single pulse.”
BackgroundRegulation of intracellular calcium concentration by nanosecond pulsed electric fields
Scarlett, S. S., White, J. A., Blackmore, P. F., Schoenbach, K. H., & Kolb, J. F. (2009) · Biochimica et Biophysica Acta (BBA) - Biomembranes · doi:10.1016/j.bbamem.2009.02.006 · Cited by 96
“Since the pulse duration is much shorter than the charging time of the cell, the interior of the cell is not yet shielded from the external field and organelle membranes are charged in a manner similar to the outer membrane [41,42].”
BackgroundSmith, K. C., & Weaver, J. C. (2008) · Biophysical Journal · doi:10.1529/biophysj.107.121921 · Cited by 94
Subnanosecond Electric Pulses Cause Membrane Permeabilization and Cell Death
Xiao, S. H., Guo, S., Nesin, V., Heller, R. F., & Schoenbach, K. H. (2011) · IEEE Transactions on Biomedical Engineering · doi:10.1109/tbme.2011.2112360 · Cited by 91
“For subnanosecond pulses, the probability of penetrating into the interior of the cell is even higher than for nanosecond pulses [19].”
BackgroundAdvantages and Disadvantages of Different Concepts of Electroporation Pulse Generation
Reberšek, M., & Damijan Miklavčič, A. (2011) · Automatika · doi:10.1080/00051144.2011.11828399 · Cited by 91
“Elektroporator je generator impulsa koji se koristi za permeabilizaciju stanica.”
BackgroundElectrochemical Control of Cell and Tissue Polarity
Chang, F., & Minc, N. (2014) · Annual Review of Cell and Developmental Biology · doi:10.1146/annurev-cellbio-100913-013357 · Cited by 87
Perspective on Pulsed Electric Field Treatment in the Bio-based Industry
Buchmann, L., & Mathys, A. (2019) · Frontiers in Bioengineering and Biotechnology · doi:10.3389/fbioe.2019.00265 · Cited by 82
Ibey, B. L., Roth, C. C., Pakhomov, A. G., Bernhard, J. A., Wilmink, G. J., & Pakhomova, O. N. (2011) · PLoS ONE · doi:10.1371/journal.pone.0015642 · Cited by 80
“apt to form pores than a larger cell due to a smaller induced transmembrane potential [2,3,45,46,47,48].”
BackgroundZhao, H.-P., Steiger, A., Nohner, M., & Ye, H. (2015) · PLoS ONE · doi:10.1371/journal.pone.0129625 · Cited by 75
“Although little is known about the effects of the DC electric field on internal organelles such as mitochondria, theoretical works have shown that the cytoplasmic membrane could provide “shielding effects” against the DC electric field, and prevent the electric field from penetrating into the cell [49].”
BackgroundPerrier, D. L., Rems, L., & Boukany, P. E. (2017) · Advances in Colloid and Interface Science · doi:10.1016/j.cis.2017.04.016 · Cited by 76
Nanosecond Pulsed Electric Field (nsPEF): Opening the Biotechnological Pandora’s Box
Ruiz-Fernández, A. R., Campos, L., Gutiérrez-Maldonado, S. E., Núñez, G., Villanelo, F., & Pérez‐Acle, T. (2022) · International Journal of Molecular Sciences · doi:10.3390/ijms23116158 · Cited by 74
Nesin, V., Bowman, A. M., Xiao, S. H., & Pakhomov, A. G. (2011) · Bioelectromagnetics · doi:10.1002/bem.21696 · Cited by 72
“Indeed, the conditions for selective intracellular electroporation appear somewhat exotic and it is not clear if they exist in living cells [Kotnik and Miklavcic, 2006].”
Sano, M. B., Fan, R. E., & Xing, L. (2017) · Scientific Reports · doi:10.1038/srep40747 · Cited by 69
Vižintin, A., Marković, S., Ščančar, J., & Damijan Miklavčič, A. (2021) · Bioelectrochemistry · doi:10.1016/j.bioelechem.2021.107798 · Cited by 68
Electroporation of Intracellular Liposomes Using Nanosecond Electric Pulses—A Theoretical Study
Rems, L., Pucihar, G., & Damijan Miklavčič, A. (2013) · IEEE Transactions on Biomedical Engineering · doi:10.1109/tbme.2013.2262177 · Cited by 68
Nanosecond electric pulses: A mini-review of the present state of the art
Chopinet, L., & Rols, M. (2014) · Bioelectrochemistry · doi:10.1016/j.bioelechem.2014.07.008 · Cited by 67
Electropermeabilization of endocytotic vesicles in B16 F1 mouse melanoma cells
Napotnik, T. B., Reberšek, M., Kotnik, T., Lebrasseur, É., Cabodevila, G., & Damijan Miklavčič, A. (2010) · Medical & Biological Engineering & Computing · doi:10.1007/s11517-010-0599-9 · Cited by 67
“Although the nsEP have strong effects on cell interior, theoretical models and simulations [21, 32, 55] point out that generally, the plasma membrane is also affected.”
BackgroundNovel Passive Element Circuits for Microdosimetry of Nanosecond Pulsed Electric Fields
Merla, C., Denzi, A., Paffi, A., Casciola, M., d'Inzeo, G., Apollonio, F., & Liberti, M. (2012) · IEEE Transactions on Biomedical Engineering · doi:10.1109/tbme.2012.2203133 · Cited by 66
Reberšek, M., Kranjc, M., Pavliha, D., Napotnik, T. B., Vrtačnik, D., Amon, S., & Damijan Miklavčič, A. (2009) · IEEE Transactions on Biomedical Engineering · doi:10.1109/tbme.2009.2027422 · Cited by 64
Effect of nanosecond pulsed electric field treatment on cell proliferation of microalgae
Buchmann, L., Frey, W., Gusbeth, C., Ravaynia, P. S., & Mathys, A. (2018) · Bioresource Technology · doi:10.1016/j.biortech.2018.09.124 · Cited by 60
“Based on mathematical models an increased electropermeabilization effect on intracellular structures compared to the cell membrane was observed for angular frequencies ω (rad s−1) above 107 (Kotnik and Miklavčič, 2006; Vernier et al., 2006).”
Xu, S., Du, C., Zhang, M., Wang, R., Feng, W., Wang, C., Liu, Q., & Zhao, W. (2023) · Nano Research · doi:10.1007/s12274-023-5527-z · Cited by 59
Teissié, J., Escoffre, J., Rols, M., & Golzio, M. (2008) · Radiology and Oncology · doi:10.2478/v10019-008-0016-2 · Cited by 59
Pulse Electric Field Technology for Wastewater and Biomass Residues’ Improved Valorization
Capodaglio, A. G. (2021) · Processes · doi:10.3390/pr9050736 · Cited by 58
“In conventional PEF processing the electric field is applied in intervals in the range of micro- to milliseconds; in nanosecond PEF (nsPEF) processing, much higher electric fields (10–100 kV/cm) are applied for intervals of 1–300 ns, inducing distinct intracellular effects from those of conventional PEF [8].”
BackgroundVariability of the Minimal Transmembrane Voltage Resulting in Detectable Membrane Electroporation
Towhidi, L., Kotnik, T., Pucihar, G., Firoozabadi, M., Mozdarani, H., & Damijan Miklavčič, A. (2008) · Electromagnetic Biology and Medicine · doi:10.1080/15368370802394644 · Cited by 58
“The conductivities of the cytoplasm and the (non porated) membrane were set at 3 10 1 S/m and 5 10 7 Sm 1, respectively, and the membrane thickness at 5 nm (Kotnik et al., 1997; Kotnik and Miklavčič, 2006).”
BackgroundMechanisms and immunogenicity of nsPEF-induced cell death in B16F10 melanoma tumors
Rossi, A., Pakhomova, O. N., Pakhomov, A. G., Weygandt, S., Bulysheva, A. A., Murray, L., Mollica, P. A., & Muratori, C. (2019) · Scientific Reports · doi:10.1038/s41598-018-36527-5 · Cited by 58
Merla, C., Paffi, A., Apollonio, F., Lévêque, P., d'Inzeo, G., & Liberti, M. (2011) · IEEE Transactions on Biomedical Engineering · doi:10.1109/tbme.2010.2104150 · Cited by 55
Advantages and Disadvantages of Different Concepts of Electroporation Pulse Generation
Reberšek, M., & Damijan Miklavčič, A. (2011) · doi:10.7305/automatika.52-1.79 · Cited by 55
Miklovic, T., Latouche, E. L., DeWitt, M. R., Davalos, R. V., & Sano, M. B. (2017) · Annals of Biomedical Engineering · doi:10.1007/s10439-017-1889-2 · Cited by 54
Assessment of Cytoplasm Conductivity by Nanosecond Pulsed Electric Fields
Denzi, A., Merla, C., Palego, C., Paffi, A., Ning, Y., Multari, C. R., Cheng, X., Apollonio, F., Hwang, J. C. M., & Liberti, M. (2015) · IEEE Transactions on Biomedical Engineering · doi:10.1109/tbme.2015.2399250 · Cited by 53
“result, three-layer microdosimetry models often resorted to the conductivity of simple phosphate buffer saline solution (PBS) [15], [19], [30], or water with a certain fraction of dissolved”
BackgroundEffects of Oscillatory Electric Fields on Internal Membranes: An Analytical Model
Vajrala, V., Claycomb, J. R., Sanabria, H., & Miller, J. H. (2007) · Biophysical Journal · doi:10.1529/biophysj.107.114611 · Cited by 51
“More recently, Kotnik and Miklavčič calculated the ac transmembrane voltage induced on spheroidal cells (26) and across internal membranes, using a double-shell model for application to cells exposed to nanosecond-duration pulsed electric fields (27).”
MethodHuang, K., Yu, L., Wang, W., Gai, L., & Wang, J. (2013) · Food Control · doi:10.1016/j.foodcont.2013.07.011 · Cited by 48
Controlling properties of human neural progenitor cells using 2D and 3D conductive polymer scaffolds
Song, S., Amores, D., Chen, C., McConnell, K. W., Oh, B., Poon, A. S. Y., & George, P. M. (2019) · Scientific Reports · doi:10.1038/s41598-019-56021-w · Cited by 49
Ye, H., Cotic, M., Kang, E. E., Fehlings, M. G., & Carlen, P. L. (2010) · Journal of NeuroEngineering and Rehabilitation · doi:10.1186/1743-0003-7-12 · Cited by 47
“Another study [41] has modeled the internal membrane response to the time-varying electric field, and has investigated the condition under which ψorg can temporarily exceed ψcell under nanosecond duration pulsed electric fields.”
InfluentialSong, J., Joshi, R. P., & Schoenbach, K. H. (2011) · Medical & Biological Engineering & Computing · doi:10.1007/s11517-011-0745-z · Cited by 48
Histopathology of normal skin and melanomas after nanosecond pulsed electric field treatment
Chen, X., Swanson, R. J., Kolb, J. F., Nuccitelli, R., & Schoenbach, K. H. (2009) · Melanoma Research · doi:10.1097/cmr.0b013e32832f1558 · Cited by 46
From the basic science of biological effects of ultrashort electrical pulses to medical therapies
Schoenbach, K. H. (2018) · Bioelectromagnetics · doi:10.1002/bem.22117 · Cited by 44
“This simple, analytical model, which served as the basis for the understanding of intracellular effects caused by ultrashort pulses, has been refined and expanded [Foster, 2000; Kotnik and Miklavcic, 2006].”
MethodMicrodosimetric Study for Nanosecond Pulsed Electric Fields on a Cell Circuit Model with Nucleus
Denzi, A., Merla, C., Camilleri, P., Paffi, A., d'Inzeo, G., Apollonio, F., & Liberti, M. (2013) · The Journal of Membrane Biology · doi:10.1007/s00232-013-9546-7 · Cited by 44
“The analytical models rely on the solution of the quasi-static Laplace equation on a spherical cell (Kotnik and Miklavcic 2006; Kotnik et al. 2010; Merla et al. 2011); the numerical ones are based on a distributed circuit representation (Joshi et al.”
BackgroundMethodInfluentialYe, H., Hendee, J., Ruan, J., Zhirova, A., Ye, J., & Dima, M. (2022) · Journal of NeuroEngineering and Rehabilitation · doi:10.1186/s12984-022-01094-4 · Cited by 46
“Computational work revealed that larger cell bodies are associated with greater transmembrane potentials under electrical [56] and magnetic [13] stimulation.”
BackgroundŽgalin, M. K., Hodžić, D., Reberšek, M., & Kandušer, M. (2012) · The Journal of Membrane Biology · doi:10.1007/s00232-012-9481-z · Cited by 43
Nanosecond Electric Pulse Effects on Gene Expression
Chopinet, L., Napotnik, T. B., Montigny, A., Reberšek, M., Teissié, J., Rols, M., & Damijan Miklavčič, A. (2013) · The Journal of Membrane Biology · doi:10.1007/s00232-013-9579-y · Cited by 43
“Numerical simulations (Joshi et al. 2004; Kotnik and Miklavcic 2006; Retelj et al. 2013) predict that the electric parameters of cells Fig.”
BackgroundWindow effect of pulsed electric field on biological cells
Yao, C., Hu, X., Mi, Y., Li, C., & Sun, C. (2009) · IEEE Transactions on Dielectrics and Electrical Insulation · doi:10.1109/tdei.2009.5293936 · Cited by 40
“Kontik and Damijan [17-19] extended the Schwan theory, introducing a general analytical method.”
BackgroundKranjc, M., Kranjc, S., Bajd, F., Serša, G., Serša, I., & Miklavčič, D. (2017) · Scientific Reports · doi:10.1038/s41598-017-10846-5 · Cited by 42
Cell membrane thermal gradients induced by electromagnetic fields
Garner, A. L., Deminsky, M., Neculaes, V. B., Chashihin, V., Knizhnik, A. A., & Potapkin, B. V. (2013) · Journal of Applied Physics · doi:10.1063/1.4809642 · Cited by 39
Membrane dielectric dispersion in nanosecond pulsed electroporation of biological cells
Salimi, E., Thomson, D. J., & Bridges, G. E. (2013) · IEEE Transactions on Dielectrics and Electrical Insulation · doi:10.1109/tdei.2013.6571442 · Cited by 39
“There is evidence that by applying megavolt-per-meter, nanosecond duration electric pulses, such that the pulse duration is less than the charging time of the cell membrane, the electric field can penetrate inside the cell and electroporate internal membranes [9-11].”
BackgroundHaberkorn, I., Buchmann, L., Hiestand, M., & Mathys, A. (2019) · Bioresource Technology · doi:10.1016/j.biortech.2019.122029 · Cited by 40
Mercadal, B., Vernier, P. T., & Ivorra, A. (2016) · The Journal of Membrane Biology · doi:10.1007/s00232-016-9907-0 · Cited by 38
“, 1988) εe 70 Relative permittivity of the extracellular medium (Kotnik and Miklavčič, 2006) εi 70 Relative permittivity of the intracellular medium (Kotnik and Miklavčič, 2006) εm 5 Relative permittivity of the membrane (Kotnik and Miklavčič, 2006) w 10 × R Dimensions of the simulated space (10 times cell radius) σp…”
BackgroundTemperature Dependence of High Frequency Irreversible Electroporation Evaluated in a 3D Tumor Model
Fesmire, C. C., Petrella, R. A., FOGLE, C. A., Gerber, D. A., Xing, L., & Sano, M. B. (2020) · Annals of Biomedical Engineering · doi:10.1007/s10439-019-02423-w · Cited by 38
Evaluation of the Electroporation Efficiency of a Grid Electrode for Electrochemotherapy
Ongaro, A., Campana, L. G., Mattei, M. D., Dughiero, F., Forzan, M., Pellati, A., Rossi, C. R., & Sieni, E. (2015) · Technology in Cancer Research & Treatment · doi:10.1177/1533034615582350 · Cited by 37
“A simple model of needles inserted in homogeneous tissue was proposed previously.(2,18,27-29,31-35) Figure 2 shows the model geometry, a parallelepiped (eg, sizes 5 3.”
MethodField Distribution and DNA Transport in Solid Tumors During Electric Field-Mediated Gene Delivery
Henshaw, J. W., & Yuan, F. (2007) · Journal of Pharmaceutical Sciences · doi:10.1002/jps.21000 · Cited by 37
Assessing how electroporation affects the effective conductivity tensor of biological tissues
Mezeme, M. E., Kranjc, M., Bajd, F., Serša, I., Brosseau, C., & Damijan Miklavčič, A. (2012) · Applied Physics Letters · doi:10.1063/1.4767450 · Cited by 37
Transmembrane potential induced in a spherical cell model under low-frequency magnetic stimulation
Ye, H., Cotic, M., & Carlen, P. L. (2007) · Journal of Neural Engineering · doi:10.1088/1741-2560/4/3/014 · Cited by 36
Ye, H., Cotic, M., Fehlings, M. G., & Carlen, P. L. (2010) · Medical & Biological Engineering & Computing · doi:10.1007/s11517-010-0704-0 · Cited by 36
“The choice of values and their ranges for the electric parameters was based on reports in the literature [23, 24].”
MethodSteuer, A., Schmidt, A., Labohá, P., Babica, P., & Kolb, J. F. (2016) · Bioelectrochemistry · doi:10.1016/j.bioelechem.2016.07.003 · Cited by 36
Huang, K., Jiang, T., Wang, W., Gai, L., & Wang, J. (2014) · Food and Bioprocess Technology · doi:10.1007/s11947-014-1272-3 · Cited by 35
The Phenomenon of Electroporation
Mahnič-Kalamiza, S., & Damijan Miklavčič, A. (2022) · Food engineering series · doi:10.1007/978-3-030-70586-2_3 · Cited by 33
“This is followed, within a microsecond, by the transmembrane field being amplified by polarization (positive and negative charge carriers accumulating either side of the membrane), resulting in the buildup of an induced transmembrane voltage (Kotnik and Miklavčič 2006).”
BackgroundGarcía-Sánchez, T., Merla, C., Fontaine, J., Muscat, A., & Mir, L. M. (2018) · Biochimica et Biophysica Acta (BBA) - Biomembranes · doi:10.1016/j.bbamem.2018.01.018 · Cited by 32
“Considering our results as a reflect of electropermeabilization of the internal membranes, the observed low pass filter frequency response is not in agreement with the theoretical predictions where a band pass filter behavior is usually assigned for the intracellular membranes [23,24].”
BackgroundMethodResultInfluentialNatural Fluctuations of an Electropore Show Fractional Lévy Stable Motion
Kotulska, M. (2006) · Biophysical Journal · doi:10.1529/biophysj.106.091363 · Cited by 32
Merla, C., Pakhomov, A. G., Semenov, I., & Vernier, P. T. (2017) · Biochimica et Biophysica Acta (BBA) - Biomembranes · doi:10.1016/j.bbamem.2017.04.014 · Cited by 31
Buchmann, L., Böcker, L., Frey, W., Haberkorn, I., Nyffeler, M., & Mathys, A. (2018) · Innovative Food Science & Emerging Technologies · doi:10.1016/j.ifset.2018.04.013 · Cited by 31
Electrolytic ablation enables cancer cell targeting through pH modulation
Perkons, N. R., Stein, E. J., Nwaezeapu, C., Wildenberg, J. C., Saleh, K. S., Itkin-Ofer, R., Ackerman, D., Soulen, M. C., Hunt, S. J., Nadolski, G., et al. (2018) · Communications Biology · doi:10.1038/s42003-018-0047-1 · Cited by 31
Metadata
- Authors
- Kotnik, T., & Miklavčič, D.
- Affiliations
- University of Ljubljana (SI)
- Corresponding author
- Kotnik, Tadej
- ORCID
- Kotnik, Tadej
- Venue
- Biophysical Journal
- Publisher
- Elsevier BV
- Published
- 2006-01
- Volume
- 90
- Issue
- 2
- Pages
- 480-491
- ISSN
- 0006-3495 (ISSN-L)
- PMC
- PMC1367054
- OpenAlex
- W2106955112
- Cited by
- 318
- References
- 68
- Citations by year
- 2026: 13 · 2025: 11 · 2024: 15 · 2023: 22 · 2022: 12 · 2021: 12 · 2020: 17 · 2019: 20 · 2018: 19 · 2017: 26 · 2016: 19 · 2015: 12 · 2014: 19 · 2013: 17 · 2012: 17
- FWCI
- 4.26
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Biophysical Journal (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biophysics › Electromagnetic Fields and Biological Effects
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biotechnology › Microbial Inactivation Methods
- Physical Sciences › Engineering › Electrical and Electronic Engineering › Electrical and Electromagnetic Research
- Keywords
- electric field effects on cells, organelle membranes, nanosecond pulses, frequency domain analysis, time-domain analysis, membrane conductivity
- MeSH terms
- Animals, Biological Transport, Active, Biophysics, Cell Membrane, Cell Membrane Permeability, Cytosol, Electric Conductivity, Electromagnetic Fields, Intracellular Membranes, Membrane Fluidity, Membrane Lipids, Membrane Potentials, Models, Theoretical, Time Factors
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
