The DOI matches its Crossref record; 33 of the 33 references it cites were found in a registry or an index.
Characterization of the Myosin-Based Source for Second-Harmonic Generation from Muscle Sarcomeres
Open accessAbstract
Several biologically important protein structures give rise to strong second-harmonic generation (SHG) in their native context. In addition to high-contrast optical sections of cells and tissues, SHG imaging can provide detailed structural information based on the physical constraints of the optical effect. In this study we characterize, by biochemical and optical analysis, the critical structures underlying SHG from the complex muscle sarcomere. SHG emission arises from domains of the sarcomere containing thick filaments, even within nascent sarcomeres of differentiating myocytes. SHG from isolated myofibrils is abolished by extraction of myosin, but is unaffected by removal or addition of actin filaments. Furthermore, the polarization dependence of sarcomeric SHG is not affected by either the proportion of myosin head domains or the orientation of myosin heads. By fitting SHG polarization anisotropy readings to theoretical response curves, we find an orientation for the elemental harmonophore that corresponds well to the pitch of the myosin rod alpha-helix along the thick filament axis. Taken together, these data indicate that myosin rod domains are the key structures giving SHG from striated muscle. This study should guide the interpretation of SHG contrast in images of cardiac and skeletal muscle tissue for a variety of biomedical applications.
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Self-citationInterpreting Second-Harmonic Generation Images of Collagen I Fibrils
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Kinesin-binding-triggered conformation switching of microtubules contributes to polarized transport
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Metadata
- Authors
- Plotnikov, S. V., Millard, A. C., Campagnola, P. J., & Mohler, W. A.
- Venue
- Biophysical Journal
- Publisher
- Elsevier BV
- Published
- 2006-01
- Volume
- 90
- Issue
- 2
- Pages
- 693-703
- ISSN
- 0006-3495 (ISSN-L)
- OpenAlex
- W1971450283
- Cited by
- 444
- References
- 33
- FWCI
- 10.14
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Plotnikov, S. V., Millard, A. C., Campagnola, P. J., & Mohler, W. A. (2006). Characterization of the Myosin-Based Source for Second-Harmonic Generation from Muscle Sarcomeres. Biophysical Journal. https://doi.org/10.1529/biophysj.105.071555
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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