Contribution from local conversion of thyroxine to 3,5,3'-triiodothyronine to intracellular 3,5,3'-triiodothyronine in several organs in hypothyroid rats at isotope equilibrium
Abstract
The intracellular conversion of T4 to T3 was investigated in various tissues of hypothyroid rats after continuous iv infusion of radiolabelled T3 and T4. Two groups of 4 thyroidectomized rats were infused with carrier-free 125I-labelled T4 as well as 131I-labelled T3 until isotope equilibrium was achieved. Plasma, various tissue homogenates (liver, kidney, pituitary, thigh muscle, cerebral cortex and cerebellum) and subcellular fractions (nuclei, mitochondria, microsomes, cytoplasm) from liver, kidney and the pituitary gland were extracted for thin layer chromatography. The [125I]T3/[131I]T3 ratios were determined and the extra contribution of [125I]T3 derived from local conversion of [125I]T4 to the total [125I]T3 was calculated in percent. In addition to the [125I]T3 derived from plasma, [125I]T3 derived from locally converted [125I]T4 was present in all tissues investigated. There was substantially more, although in varying quantities, in the cerebral cortex (79 ± 2%), the cerebellum (68 ± 4%) and the pituitary gland (53 ± 1%) than in the liver (10 ± 6%), the kidney (11 ± 5%) and thigh muscle (17 ± 6%); in the latter tissues most of the 125I-labelled T3 is derived directly from plasma. These results indicate that in the brain of severe hypothyroid rats there is pronounced conversion of T4 to T3 and effective binding of the T3 produced whereas the T3 in the liver, kidney, and muscle is predominantly derived from plasma. At the intracellular level, within the investigated ti…
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In rat liver, plasma-derived T3 and T3 generated locally from T4 via deiodinase type 1 (D1) are in different compartments with respect to nuclear receptor binding; practically all of the nuclear T3 appears to be plasma derived.
“In rat liver, for example, plasma-derived T3 and T3 generated locally from T4 via deiodinase type 1 (D1) are in different compartments with respect to nuclear receptor binding: practically all of the nuclear T3 appears to be plasma derived [31] .”
From 2012 ETA Guidelines: The Use of L-T4 + L-T3 in the Treatment of Hypothyroidism (2012) · cites this paper as [31]
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- Authors
- van Doom, J., Roelfsema, F., & van der Heide, D.
- ORCID
- Roelfsema, F.
- Venue
- Acta Endocrinologica
- Publisher
- Oxford University Press (OUP)
- Published
- 1982-11
- Volume
- 101
- Issue
- 3
- Pages
- 386-396
- ISSN
- 0804-4643 (ISSN-L)
- 1479-683X
- OpenAlex
- W2134694041
- Cited by
- 50
- References
- 0
- Citations by year
- 2022: 1 · 2017: 2 · 2016: 1 · 2015: 2 · 2014: 1 · 2012: 1
- FWCI
- 4.35
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- European Journal of Endocrinology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- University of Pittsburgh
- Topics
- Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Thyroid Disorders and Treatments
- Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Thyroid Cancer Diagnosis and Treatment
- Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Growth Hormone and Insulin-like Growth Factors
- Keywords
- thyroxine to triiodothyronine conversion, hypothyroidism, thyroidectomy, isotope equilibrium, subcellular localization, brain, pituitary gland, liver, kidney, muscle, cerebellum, cerebral cortex
- MeSH terms
- Animals, Brain, Hypothyroidism, Kidney, Liver, Male, Muscles, Pituitary Gland, Rats, Inbred Strains, Research Design, Subcellular Fractions, Thyroidectomy, Thyroxine, Triiodothyronine, Rats
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