The DOI matches its Crossref record; 33 of the 33 references it cites were found in a registry or an index.
Effect of long-term ovariectomy and estrogen replacement on the expression of estrogen receptor gene in female rats
Abstract
OBJECTIVE: Estrogen exerts a wide variety of actions involving many target tissues. We studied the effects of long-term ovariectomy (OVX) and OVX with 17beta-estradiol treatment (OVXE2) on the level of estrogen receptor (ER) gene expression in target tissues of female rats. DESIGN: Three groups of Sprague-Dawley female rats were utilized in this study: sham operated (SO), OVX and OVXE2. METHODS: SO and OVX were performed 2 weeks before starting the 17beta-estradiol treatment. All groups were maintained on liquid diet for 12 weeks from the time of estradiol treatment. Total RNA was prepared from the tissues of the rats and relative quantitative reverse transcription PCR was utilized to compare the ER alpha-subtype (ERalpha) mRNA level in the three groups for each target tissue. RESULTS: Following long-term OVX, the levels of ERalpha expression showed a significant increase in the uterus, kidney and cerebral cortex and no significant change in the liver, cerebellum, brainstem, heart and thoracic and abdominal aorta compared with their SO levels. On the other hand, a 12-week treatment of OVX rats with 17beta-estradiol restored the previously upregulated ERalpha mRNA to near SO levels except for the liver where the 17beta-estradiol treatment resulted in a significant increase in the ERalpha mRNA level compared with that in SO rats. CONCLUSIONS: We conclude that the regulation of ERs by its ligand is tissue specific.
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“Since long-term hormone withdrawal is known to alter hormone receptor levels [122, 161], it seems more likely that following quiescence in primates, hormones transiently have different effects on the circadian system than those observed in species with low-level steroidogenesis throughout the juvenile period.”
From The neuroendocrine control of the circadian system: Adolescent chronotype (2012) · cites this paper as [122]
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Metadata
- Authors
- Mohamed, M., & Abdel-Rahman, A.
- Affiliations
- East Carolina University (US)
- Venue
- European Journal of Endocrinology
- Publisher
- Oxford University Press (OUP)
- Published
- 2000-03-01
- Volume
- 142
- Issue
- 3
- Pages
- 307-314
- ISSN
- 0804-4643 (ISSN-L)
- 1479-683X
- OpenAlex
- W2145224758
- Cited by
- 127
- References
- 33
- Citations by year
- 2025: 3 · 2024: 4 · 2023: 5 · 2022: 5 · 2021: 2 · 2020: 8 · 2019: 5 · 2018: 4 · 2017: 5 · 2016: 4 · 2015: 6 · 2014: 5 · 2013: 7 · 2012: 8
- FWCI
- 3.31
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- European Journal of Endocrinology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health (5r01aa010257-03)
- National Institute on Alcohol Abuse and Alcoholism (AA-10257)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Endocrinology › Estrogen and related hormone effects
- Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Menopause: Health Impacts and Treatments
- Health Sciences › Nursing › Nutrition and Dietetics › Phytoestrogen effects and research
- Keywords
- estrogen receptor alpha, ovariectomy, estrogen replacement therapy, estradiol, tissue-specific gene expression, uterus, kidney, cerebral cortex, liver, female rats
- MeSH terms
- Animals, Cerebral Cortex, Estradiol, Estrogens, Female, Gene Expression Regulation, Kidney, Ovariectomy, Receptors, Estrogen, Uterus, Weight Gain, Rats, Sprague-Dawley, Reverse Transcriptase Polymerase Chain Reaction, Rats
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