Regulation of pituitary inhibin/activin subunits and follistatin gene expression by GnRH in female rats
Open accessAbstract
Pituitary inhibin B, activin B, and follistatin are local regulators of FSH. Activin B is a homodimeric molecule (β(B)-β(B)), while inhibin B contains an α and a β(B) subunit. The regulation of gene expression of α, β(B), and follistatin by local and endocrine hormones was examined in pituitaries from female rats and in perifused pituitary cells by RT-PCR. Ovariectomy (OVX) induced an elevation in the mRNA level of α and β(B) subunits and follistatin. Short-term (4 h) treatment of pituitary cells with GnRH decreased both the inhibin α and the inhibin/activin β(B) subunit mRNA levels, while long-term treatment (20 h) with 100 nM GnRH stimulated the expression of both subunits. In contrast, the mRNA level of follistatin was elevated after the short-term GnRH treatment. Long-term exposure of pituitary cells to estradiol and inhibin B suppressed the mRNA expression of β(B) and had no effect on the expression of α subunit and follistatin. Our results demonstrate that the increased expressions of inhibin/activin subunits and follistatin in the post-OVX period can be induced by the lack of gonadal negative feedback, resulting in a high GnRH environment in the pituitary. This study reports for the first time that GnRH administered in high doses and for a long period stimulates the gene expression of inhibin/activin subunits and thereby may contribute to the stimulatory effect of OVX on the expression of these genes.
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An estrogen modified feedback mechanism may act at the pituitary level.
“An estrogen modified feedback mechanism may act at this level (Popovics et al., 2011;Walf and Frye, 2010).”
From RETRACTED: Long term toxicity of a Roundup herbicide and a Roundup-tolerant genetically modified maize (2012) · cites this paper as Popovics et al., 2011
An estrogen-modified feedback mechanism may act at this level.
“An estrogen-modified feedback mechanism may act at this level [65,66].”
From Republished study: long-term toxicity of a Roundup herbicide and a Roundup-tolerantgenetically modified maize (2014) · cites this paper as [65]
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- Authors
- Popovics, P., Rekasi, Z., Stewart, A. J., & Kovacs, M.
- Affiliations
- University of St Andrews (GB)
- Venue
- Journal of Endocrinology
- Publisher
- Bioscientifica
- Published
- 2011-04-13
- Volume
- 210
- Issue
- 1
- Pages
- 71-79
- ISSN
- 0022-0795 (ISSN-L)
- 1479-6805
- OpenAlex
- W2152296270
- Cited by
- 13
- References
- 48
- Citations by year
- 2022: 2 · 2018: 3 · 2015: 2 · 2014: 3 · 2012: 3
- FWCI
- 2.14
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Journal of Endocrinology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Reproductive Medicine › Hypothalamic control of reproductive hormones
- Health Sciences › Medicine › Public Health, Environmental and Occupational Health › Reproductive Biology and Fertility
- Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Growth Hormone and Insulin-like Growth Factors
- Keywords
- gonadotropin-releasing hormone, activin B, follistatin, inhibin alpha subunit, gene expression regulation, ovariectomy, FSH regulation, estradiol, pituitary cells, female rats
- MeSH terms
- Animals, Cells, Cultured, Estradiol, Female, Gene Expression Regulation, Inhibins, Gonadotropin-Releasing Hormone, Ovariectomy, Pituitary Gland, Receptors, LHRH, RNA, Messenger, Rats, Wistar, Reverse Transcriptase Polymerase Chain Reaction, Protein Subunits, Inhibin-beta Subunits, Activins, Follistatin, Rats
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