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Cited by 122Growth Hormone and Insulin-like Growth FactorsReproductive Physiology in LivestockAnimal health and immunology

Negative energy balance in dairy cows is associated with specific changes in IGF-binding protein expression in the oviduct

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Fenwick, M A, Llewellyn, S, Fitzpatrick, R, Kenny, D A, Murphy, J J, Patton, J, Wathes, D C·2008·Reproduction
DOI 10.1530/rep-07-0243PMC PMC2756008
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Abstract

Negative energy balance (NEB) during early lactation in dairy cows leads to an altered metabolic state that has major effects on the production of IGF family members. Low IGF-I concentrations are associated with poor fertility and therefore we aimed to determine whether NEB exerts a direct effect on IGF expression in the postpartum oviduct. Multiparous Holstein cows were allocated to two treatments (each n=6) designed using differential feeding and milking regimes to produce either mild NEB (MNEB) or severe NEB (SNEB). Animals were slaughtered in week 2 of lactation when divergent metabolic profiles were evident. Oviducts were collected for RNA analysis by real-time RT-PCR and in situ hybridisation. Quantitative measures in oviduct gene expression were obtained for all members of the IGF family (IGF-I/II, IGF-binding proteins (IGFBP) 1-6 and receptors for IGF types 1 and 2), insulin A/B, GH, glucocorticoid and oestrogen alpha/beta. Expression of IGFBP-2 and IGFBP-6 (both of which have a high affinity for IGF-II) was decreased in SNEB relative to MNEB (P<0.05). No other gene was altered by NEB, but IGF-II, IGFBP-3, IGFBP-5 and IGFBP-6 all showed differential expression in different regions of the oviduct. These results indicate that, in addition to low circulating IGF-I after calving, NEB may also influence IGF availability in the oviduct indirectly through changes in specific IGFBP expression. It is possible that the predicted increased signalling by IGF-II may perturb embryo development, contributing to the high rates of embryonic mortality in dairy cows.

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  • BHBA and NEFA serve as indicators of body fat mobilization and negative energy balance in adult dairy cattle and are interpreted similarly in calves.

    “these parameters serve as indicators of body fat mobilization and negative energy balance in adult dairy cattle and are interpreted similarly in calves”

    From A randomized controlled trial investigating the effect of transport duration and age at transport on surplus dairy calves: Part II. Impact on hematological variables. (2023) · cites this paper as Fenwick et al., 2008

    The Results section (“Metabolic and hormonal effects of NEB”) reports elevated NEFA and β-hydroxybutyrate (BHB, also called BHBA) in cows with severe negative energy balance and describes the blood metabolite data as evidence of body tissue mobilisation. The study examined postpartum adult dairy cows, not calves, so it does not support interpreting these measures similarly in calves. (LLM, conf=0.88)

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  • The relationship between energy balance and NEFA and BHBA has primarily been studied in adult dairy cattle, in which both increase as a result of catabolism and fat mobilization.

    “The relationship between energy balance and NEFA and BHBA has primarily been studied in adult dairy cattle, in which both increase as a result of catabolism and fat mobilization”

    From A randomized controlled trial investigating the effect of transport duration and age at transport on surplus dairy calves: Part II. Impact on hematological variables. (2023) · cites this paper as Fenwick et al., 2008

    The Results (Metabolic and hormonal effects of NEB; page not provided) report that cows with severe negative energy balance had greater body-tissue mobilisation and elevated NEFA and BHB compared with cows with mild negative energy balance. Materials and Methods (Animals and tissue collection; page not provided) identifies the cows as multiparous. The paper does not establish that this relationship has primarily been studied in adult dairy cattle rather than other populations. (LLM, conf=0.85)

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Research works printed but not linked: 1

  1. Taylor VJ, Beever DE & Wathes DC 2003 Physiological adaptations to milk production that affect fertility in high yielding dairy cows. British Society of Animal Science Occasional Publication, vol 29, pp. 37–71. Nottingham, UK: Nottingham University Press.

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Authors
Fenwick, M. A., Llewellyn, S., Fitzpatrick, R., Kenny, D. A., Murphy, J. J., Patton, J., & Wathes, D. C.
Affiliations
  • Royal Veterinary College (GB)
  • University College Dublin (IE)
Corresponding author
Wathes, D C
ORCID
Venue
Reproduction
Publisher
Oxford University Press (OUP)
Published
2008-01-01
Volume
135
Issue
1
Pages
63-75
ISSN
  • 1470-1626 (ISSN-L)
  • 1741-7899
DOI
10.1530/rep-07-0243
PMC
PMC2756008
OpenAlex
W2054923638
Cited by
122
References
80
Citations by year
2026: 3 · 2025: 5 · 2024: 6 · 2023: 3 · 2022: 11 · 2021: 9 · 2020: 6 · 2019: 7 · 2018: 15 · 2017: 7 · 2016: 8 · 2015: 3 · 2014: 4 · 2013: 5 · 2012: 14
FWCI
2.68
Type
journal article
Language
en
Open access
Licence
academic.oup.com/pages/standard-publication-reuse-rights (vor)
Locations
  • Reproduction (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
  • ARAN (University of Galway Research Repository) (Ollscoil na Gaillimhe – University of Galway) (acceptedVersion, public-domain)
  • RVC Research Online (Royal Veterinary College) (submittedVersion, cc-by)
  • Arrow@dit (Dublin Institute of Technology) (submittedVersion)
  • ARAN (University of Galway Research Repository) (Ollscoil na Gaillimhe – University of Galway) (submittedVersion, cc-by-nc-nd)
  • ARAN (University of Galway Research Repository) (Ollscoil na Gaillimhe – University of Galway) (submittedVersion, cc-by-nc)
  • Research Repository UCD (University College Dublin) (cc-by-nc)
Funders
  • Wellcome Trust (072315)
  • Teagasc
Topics
  • Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Growth Hormone and Insulin-like Growth Factors
  • Life Sciences › Agricultural and Biological Sciences › Agronomy and Crop Science › Reproductive Physiology in Livestock
  • Health Sciences › Veterinary › Small Animals › Animal health and immunology
Keywords
negative energy balance, dairy cattle, IGF binding proteins, oviduct, insulin-like growth factor II, insulin-like growth factor I, IGFBP-2, IGFBP-6, early lactation, postpartum metabolism, embryonic development, embryonic mortality, IGF receptors, insulin, growth hormone, glucocorticoids, estrogen
MeSH terms
Animals, Autoradiography, Cattle, Dairying, Energy Metabolism, Fallopian Tubes, Female, Fetal Death, Insulin-Like Growth Factor I, Insulin-Like Growth Factor II, Lactation, Pregnancy, Receptors, Somatotropin, RNA, Messenger, Signal Transduction, Gene Expression, In Situ Hybridization, Insulin-Like Growth Factor Binding Proteins, Insulin-Like Growth Factor Binding Protein 2, Insulin-Like Growth Factor Binding Protein 6, Reverse Transcriptase Polymerase Chain Reaction

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