Nicotine effect on bone remodeling during orthodontic tooth movement: Histological study in rats
Open accessAbstract
Introduction: Nicotine is harmful to angiogenesis, osteogenesis and synthesis of collagen. Objective: The aim of this study was to investigate the effect of nicotine on bone remodeling during orthodontic movement in rats. Methods: Eighty male Wistar rats were randomly divided into three groups: Group C (control), group CM (with orthodontic movement) and group NM (nicotine with orthodontic movement) groups. The animals comprising groups C and CM received 0.9% saline solution while group NM received nicotine solution (2 mg/kg). A nickel-titanium closed-coil spring was used to induce tooth movement. The animals were euthanized and tissue specimens were processed histologically. We quantified blood vessels, Howship's lacunae and osteoclast-like cells present in the tension and compression areas of periodontal ligaments. The extent of bone formation was evaluated under polarized light to determine the percentage of immature/mature collagen. Results: We observed lower blood vessel densities in the NM group in comparison to the CM group, three (p < 0.001) and seven (p < 0.05) days after force application. Osteoclast-like cells and Howship's lacunae in the NM group presented lower levels of expression in comparison to the CM group, with significant differences on day 7 (p < 0.05 for both variables) and day 14 (p < 0.05 for osteoclast-like cells and p < 0.01 for Howship's lacunae). The percentage of immature collagen increased in the NM group in comparison to the CM group with a statistically significant difference on day 3 (p < 0.05), day 7 (p < 0.001), day 14 (p < 0.001) and day 21 (p < 0.001). Conclusions: Nicotine affects bone remodeling during orthodontic movement, reducing angiogenesis, osteoclast-like cells and Howship's lacunae, thereby delaying the collagen maturation process in developed bone matrix.
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Nicotine has no influence on osteoclastogenesis.
“Similarly, Shintcovsk and colleagues demonstrated that nicotine had no influence on osteoclastogenesis [68].”
From An Overlooked Bone Metabolic Disorder: Cigarette Smoking-Induced Osteoporosis (2022) · cites this paper as [68]
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An Overlooked Bone Metabolic Disorder: Cigarette Smoking-Induced Osteoporosis
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- Authors
- Shintcovsk, R. L., Knop, L., Tanaka, O. M., & Maruo, H.
- Affiliations
- Brazilian Dental Association
- Pontifícia Universidade Católica do Paraná (BR)
- Universidade Estadual de Campinas (UNICAMP) (BR)
- Corresponding author
- Shintcovsk, Ricardo Lima
- Venue
- Dental Press Journal of Orthodontics
- Publisher
- FapUNIFESP (SciELO)
- Published
- 2014-04
- Volume
- 19
- Issue
- 2
- Pages
- 96-107
- ISSN
- 2176-9451 (ISSN-L)
- PMC
- PMC4296601
- OpenAlex
- W2096352925
- Cited by
- 24
- References
- 28
- Citations by year
- 2026: 1 · 2025: 1 · 2024: 2 · 2023: 2 · 2022: 2 · 2021: 3 · 2020: 1 · 2019: 3 · 2018: 5 · 2017: 2 · 2016: 1 · 2014: 1
- FWCI
- 1.95
- Type
- journal article
- Language
- en
- Open access
- diamond
- doi.org
- Licence
- cc-by-nc
- Locations
- Dental Press Journal of Orthodontics (publishedVersion, cc-by-nc)
- Europe PMC (PubMed Central) (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Americanae (AECID Library) (publishedVersion, other-oa)
- LA Referencia (Red Federada de Repositorios Institucionales de Publicaciones Científicas) (publishedVersion, other-oa)
- Americanae (AECID Library) (submittedVersion, other-oa)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Topics
- Health Sciences › Dentistry › Orthodontics › Orthodontics and Dentofacial Orthopedics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bone Metabolism and Diseases
- Health Sciences › Dentistry › Periodontics › Oral microbiology and periodontitis research
- Keywords
- nicotine, orthodontic tooth movement, bone remodeling, periodontal ligament, angiogenesis, osteoclast-like cells, Howship's lacunae, collagen maturation, bone formation, blood vessel density
- MeSH terms
- Alveolar Process, Animals, Bone Resorption, Collagen, Dental Alloys, Dental Cementum, Male, Molar, Nickel, Nicotine, Orthodontic Wires, Osteoclasts, Osteogenesis, Periodontal Ligament, Random Allocation, Stress, Mechanical, Time Factors, Titanium, Tooth Movement Techniques, Tooth Root, Bone Remodeling, Rats, Wistar, Neovascularization, Physiologic, Rats
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