The DOI matches its Crossref record; 21 of the 21 references it cites were found in a registry or an index.
Diagnosis of Streptococcus pneumoniae meningitis by polymerase chain reaction amplification of the gene for pneumolysin
Open accessAbstract
Diagnosis of bacterial meningitis has long been based on classical methods of Gram stain, serological tests, and culture of cerebrospinal fluid (CSF). The performance of these methods, especially culture and direct smear is thwarted by failure to detect bacteria following administration of antimicrobial agents and reluctance to performance lumbar punctures at admission. Indeed, patients with meningitis frequently receive antibiotics orally or by injection before the diagnosis is suspected or established Thus an alternative method has become necessary to help clinicians and epidemiologists to management and control of bacterial meningitis. We evaluate the application of a polymerase chain reaction-based (PCR) assay for amplification of pneumolysin gene (ply) to diagnosis of Streptococcus pneumoniae meningitis. The PCR assay sensitivity for CSF was 96% (95% confidence interval, CI, 90-99%) compared to a sensitivity of 59% for culture (95% CI 49-69%), 66% for Gram stain (95% CI 56-74%), and 78% for latex agglutination test (95% CI 69-86%); PCR specificity was 100% (95% CI 83-100%). PCR results were available within 4 h of the start of the assay. This molecular approach proved to be reliable and useful to identify this bacterium compared with other classical laboratory methods for identification of bacterial meningitis pathogens.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
The source backs part of the claim, not all of it — or backs it for a different population, condition or comparison.
In the citing paper
“The reported sensitivity of PCR for pneumococcal meningitis is high and reached 100% in the majority of conducted studies (median of 100%, range 79%–100%) (Table 1; see also Table S1 in the supplemental material) (49–58).”
From Molecular diagnostics in cerebrospinal fluid for the diagnosis of central nervous system infections (2024) · cites this paper as [56]
Experimental — accuracy not yet measured
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Corless, C. E., Guiver, M., Borrow, R., Edwards-Jones, V., Fox, A. J., & Kaczmarski, E. B. (2001) · Journal of Clinical Microbiology · doi:10.1128/jcm.39.4.1553-1558.2001 · Cited by 598
Kanegaye, J. T., Soliemanzadeh, P., & Bradley, J. S. (2001) · Pediatrics · doi:10.1542/peds.108.5.1169 · Cited by 370
Early treatment with parenteral penicillin in meningococcal disease.
Cartwright, K., Reilly, S., White, D., & Stuart, J. (1992) · BMJ · doi:10.1136/bmj.305.6846.143 · Cited by 265
Walker, J. A., Allen, R. L., Falmagne, P., Johnson, M. K., & Boulnois, G. J. (1987) · Infection and Immunity · doi:10.1128/iai.55.5.1184-1189.1987 · Cited by 230
The role of pneumolysin in pneumococcal pneumonia and meningitis
Hirst, R. A., Kadioglu, A., O’Callaghan, C., & Andrew, P. W. (2004) · Clinical & Experimental Immunology · doi:10.1111/j.1365-2249.2004.02611.x · Cited by 214
Leimkugel, J., Forgor, A. A., Gagneux, S., Pflüger, V., Flierl, C., Awine, E., Martin, N., Dangy, J., Smith, T., Hodgson, A., et al. (2005) · The Journal of Infectious Diseases · doi:10.1086/431151 · Cited by 214
Chatelet, I. P. D., Traore, Y., Gessner, B. D., Antignac, A., Naccro, B., Njanpop-Lafourcade, B.-M., Ouedraogo, M. S., Tiendrebeogo, S. R., Varon, E., & Taha, M. K. (2005) · Clinical Infectious Diseases · doi:10.1086/426436 · Cited by 158
Salo, P., Örtqvist, Å., & Leinonen, M. (1995) · The Journal of Infectious Diseases · doi:10.1093/infdis/171.2.479 · Cited by 155
Borrow, R., Claus, H., Guiver, M., Smart, L. E., JONES, D. M., Kaczmarski, E. B., Frosch, M., & FOX, A. J. (1997) · Epidemiology and Infection · doi:10.1017/s0950268896007261 · Cited by 129
Virolainen, A., Salo, P., Jero, J., et al. (1994) · Journal of Clinical Microbiology · doi:10.1128/jcm.32.11.2667-2670.1994 · Cited by 106
Research works printed but not linked: 4
- Clarke SC, 1993, Guidance for requests and interpretation of services provided by the Scottish Meningococcus and Pneumococcus Reference Laboratory, SCIEH Weekly Report, 37, 1
- 2002, Meningite por Pneumococo, 621
- Kaczmarski EB, 1995, Control of meningococcal disease: guidance for microbiologists, Commun Dis Rep Rev, 5, R196
- Libman E, 1905, A pneumococcus producing a peculiar form of hemolysis, Proc NY Pathol Soc, 5, 168
Cited by
Sorted by citations
Molecular diagnostics in cerebrospinal fluid for the diagnosis of central nervous system infections
Olie, S. E., Andersen, C. Ø., van de Beek, D., & Brouwer, M. C. (2024) · Clinical Microbiology Reviews · doi:10.1128/cmr.00021-24 · Cited by 38
“Controls usually consisted of bacterial meningitis cases due to other pathogens and reported specificity compared to culture was between 93% and 100% with a median of 98% (50, 52, 53, 55, 56).”
Metadata
- Authors
- Matos, J. D. A., Madureira, D. J., Rebelo, M. C., Hofer, C. B., & Barroso, D. E.
- Affiliations
- Universidade Federal do Rio de Janeiro (BR)
- Fundação Oswaldo Cruz (BR)
- Instituto Estadual de Infectologia São Sebastião, Brasil
- ORCID
- Hofer, Cristina B
- Venue
- Memórias do Instituto Oswaldo Cruz
- Publisher
- FapUNIFESP (SciELO)
- Published
- 2006-08
- Volume
- 101
- Issue
- 5
- Pages
- 559-563
- ISSN
- 0074-0276 (ISSN-L)
- OpenAlex
- W2060512313
- Cited by
- 12
- References
- 21
- Citations by year
- 2024: 2 · 2023: 1 · 2022: 1 · 2019: 1 · 2015: 1 · 2012: 2
- FWCI
- 0.46
- Type
- journal article
- Language
- en
- Open access
- diamond
- doi.org
- Licence
- cc-by-nc
- Locations
- Memórias do Instituto Oswaldo Cruz (publishedVersion, cc-by-nc)
- PubMed (publishedVersion)
- LA Referencia (Red Federada de Repositorios Institucionales de Publicaciones Científicas) (publishedVersion, other-oa)
- LA Referencia (Red Federada de Repositorios Institucionales de Publicaciones Científicas) (submittedVersion, other-oa)
- Bioline International (Bioline International) (submittedVersion)
- Americanae (AECID Library) (submittedVersion, other-oa)
- Arca - Repositório Institucional da Fiocruz (submittedVersion, other-oa)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Topics
- Health Sciences › Medicine › Microbiology (medical) › Bacterial Identification and Susceptibility Testing
- Life Sciences › Immunology and Microbiology › Microbiology › Bacterial Infections and Vaccines
- Health Sciences › Medicine › Epidemiology › Pneumonia and Respiratory Infections
- Keywords
- Streptococcus pneumoniae, bacterial meningitis, PCR diagnosis, cerebrospinal fluid, molecular diagnosis, sensitivity and specificity, latex agglutination test, antibiotic pretreatment
- MeSH terms
- Adolescent, Adult, Aged, Bacterial Proteins, Child, Child, Preschool, DNA, Bacterial, Female, Humans, Immunoenzyme Techniques, Infant, Male, Meningitis, Pneumococcal, Middle Aged, Predictive Value of Tests, Sensitivity and Specificity, Streptococcus pneumoniae, Streptolysins, Polymerase Chain Reaction, Culture Techniques
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
