Black Soldier Fly (Diptera: Stratiomyidae) Larvae Reduce Escherichia coli in Dairy Manure
Abstract
Escherichia coli labeled with a green fluorescent protein was inoculated into sterile dairy manure at 7.0 log cfu/g. Approximately 125 black soldier fly larvae were placed in manure inoculated and homogenized with E. coli. Manure inoculated with E. coli but without black soldier fly larvae served as the control. For the first experiment, larvae were introduced into 50, 75, 100, or 125 g sterilized dairy manure inoculated and homogenized with E. coli and stored 72 h at 27 degrees C. Black soldier fly larvae significantly reduced E. coli counts in all treatments. However, varying the amount of manure provided the black soldier fly larvae significantly affected their weight gain and their ability to reduce E. coli populations present. For the second experiment, larvae were introduced into 50 g manure inoculated with E. coli and stored for 72 h at 23, 27, 31, or 35 degrees C. Minimal bacterial growth was recorded in the control held at 35 degrees C and was excluded from the analysis. Black soldier fly larvae significantly reduced E. coli counts in manure held at remaining temperatures. Accordingly, temperature significantly influenced the ability of black soldier fly larvae to develop and reduce E. coli counts with greatest suppression occurring at 27 degrees C.
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Rearing BSFL at the optimum temperature improves their ability to reduce Escherichia coli.
“Rearing BSFL at the optimum temperature improves their ability to reduce Escherichia coli [32].”
From Potential Applications of Frass Derived from Black Soldier Fly Larvae Treatment of Food Waste: A Review (2022) · cites this paper as [32]
BSFL can reduce Escherichia coli and Salmonella spp. in the initial substrate.
“Previous studies have shown that BSFL can reduce Escherichia coli [32,36] and Salmonella spp. [55] in the initial substrate;”
From Potential Applications of Frass Derived from Black Soldier Fly Larvae Treatment of Food Waste: A Review (2022) · cites this paper as [32]
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“(Diptera: Muscidae) [3]; reduction of pathogens [4,5], odors [6], and antibiotics [7]; and concerns with antibiotic-resistance in the”
BackgroundSelf-citation
- Authors
- Liu, Q., Tomberlin, J. K., Brady, J. A., Sanford, M. R., & Yu, Z.
- Affiliations
- Huazhong Agricultural University (CN)
- Texas A&M University (US)
- Corresponding author
- Liu, Qiaolin
- Tomberlin, Jeffery K.
- Sanford, Michelle R.
- Venue
- Environmental Entomology
- Publisher
- Oxford University Press (OUP)
- Published
- 2008-12-01
- Volume
- 37
- Issue
- 6
- Pages
- 1525-1530
- ISSN
- 0046-225X (ISSN-L)
- OpenAlex
- W2173845026
- Cited by
- 251
- References
- 0
- Citations by year
- 2026: 7 · 2025: 9 · 2024: 27 · 2023: 20 · 2022: 34 · 2021: 28 · 2020: 28 · 2019: 29 · 2018: 23 · 2017: 11 · 2016: 10 · 2015: 6 · 2014: 2 · 2013: 11 · 2012: 1
- FWCI
- 1.73
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Environmental Entomology (publishedVersion)
- PubMed (publishedVersion)
- BioOne Complete (BioOne) (submittedVersion)
- Funders
- Texas AgriLife Research
- Cooperative State Research, Education, and Extension Service
- College of Agriculture and Life Sciences, Texas A and M University
- Topics
- Life Sciences › Agricultural and Biological Sciences › Insect Science › Insect Utilization and Effects
- Life Sciences › Agricultural and Biological Sciences › Insect Science › Forensic Entomology and Diptera Studies
- Life Sciences › Agricultural and Biological Sciences › Soil Science › Composting and Vermicomposting Techniques
- Keywords
- Hermetia illucens, dairy manure, Escherichia coli, larval rearing, manure temperature, larval weight gain, manure management
- MeSH terms
- Animals, Cattle, Diptera, Escherichia coli, Larva, Manure
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