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SV40-lnduced Immortalization of Human Cells
Abstract
For several decades simian virus 40 (SV40) early region genes have been used as a means of generating immortalized human cell lines; however, the molecular mechanisms of this process have begun to be understood only recently. SV40-induced immortalization proceeds via two phases. In the first phase ("lifespan extension"), cells continue proliferating for a limited number of population doublings beyond the point at which normal cells undergo senescence. This is mainly due to the ability of SV40 large T antigen (LTAg) to bind to the protein products of the p53 and retinoblastoma (Rb) genes. The second phase ("immortalization") occurs in only a small minority of cells, and cell hybridization analyses indicate that this is a gene inactivation event. The gene or genes involved are currently unknown, but chromosomal localization data are accumulating which should make their cloning and characterization possible in the near future.
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“SV40 T antigen is currently used for cell transfection in different types of cells and can generate immortal cell lines by binding and disabling p53 and Rb proteins [66].”
From Cell Immortality: In Vitro Effective Techniques to Achieve and Investigate Its Applications and Challenges (2024) · cites this paper as [66]
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Cited by
DAP kinase activates a p19ARF/p53-mediated apoptotic checkpoint to suppress oncogenic transformation
Raveh, T., Droguett, G., Horwitz, M. S., DePinho, R. A., & Kimchi, A. (2001) · Nature Cell Biology · doi:10.1038/35050500 · Cited by 361
Qi, Y., Wang, J. K. T., McMillian, M., & Chikaraishi, D. M. (1997) · The Journal of Neuroscience · doi:10.1523/jneurosci.17-04-01217.1997 · Cited by 330
Five dysfunctional telomeres predict onset of senescence in human cells
Kaul, Z., Cesare, A. J., Huschtscha, L. I., Neumann, A. A., & Reddel, R. R. (2012) · The EMBO Reports · doi:10.1038/embor.2011.227 · Cited by 225
“…have shown that p53 is involved in cellular senescence and that the ability of simian virus 40 to extend the proliferative life span is dependent on its T-antigen (LT) protein inactivating p53 and Rb; the period of extended life span for LT-expressing cells ends in crisis (Bryan & Reddel, 1994).”
Gire, V., & Wynford-Thomas, D. (1998) · Molecular and Cellular Biology · doi:10.1128/mcb.18.3.1611 · Cited by 161
Bond, J. A., Haughton, M. F., Rowson, J. M., Smith, P. J., Gire, V., Wynford-Thomas, D., & Wyllie, F. S. (1999) · Molecular and Cellular Biology · doi:10.1128/mcb.19.4.3103 · Cited by 104
MacKenzie, K. L., Franco, S., May, C., Sadelain, M., & Moore, M. A. S. (2000) · Experimental Cell Research · doi:10.1006/excr.2000.4982 · Cited by 92
Chalak, M., Hesaraki, M., Mirbahari, S. N., Yeganeh, M., Abdi, S., Rajabi, S., & Hemmatzadeh, F. (2024) · Life · doi:10.3390/life14030417 · Cited by 66
Burmeister, B., Schwerdtle, T., Poser, I., Hoffmann, E., Hartwig, A., Müller, W.-U., Rettenmeier, A. W., Seemayer, N. H., & Dopp, E. (2004) · Mutation Research/Genetic Toxicology and Environmental Mutagenesis · doi:10.1016/j.mrgentox.2003.11.003 · Cited by 52
Cell Immortalization: In Vivo Molecular Bases and In Vitro Techniques for Obtention
de Bardet, J. C., Cardentey, C. R., González, B. L., Patrone, D., Mulet, I. L., Siniscalco, D., & Robinson-Agramonte, M. D. L. A. (2023) · BioTech · doi:10.3390/biotech12010014 · Cited by 35
Metadata
- Authors
- Bryan, T., & Redder, R. R.
- Venue
- Critical Reviews™ in Oncogenesis
- Publisher
- Begell House
- Published
- 1994
- Volume
- 5
- Issue
- 4
- Pages
- 331-357
- ISSN
- 0893-9675
- Cited by
- 170
- References
- 0
- Language
- en
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