The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Reprogramming Efficiency Following Somatic Cell Nuclear Transfer Is Influenced by the Differentiation and Methylation State of the Donor Nucleus
Open accessAbstract
Reprogramming of a differentiated cell nucleus by somatic cell nuclear transplantation is an inefficient process. Following nuclear transfer, the donor nucleus often fails to express early embryonic genes and establish a normal embryonic pattern of chromatin modifications. These defects correlate with the low number of cloned embryos able to produce embryonic stem cells or develop into adult animals. Here, we show that the differentiation and methylation state of the donor cell influence the efficiency of genomic reprogramming. First, neural stem cells, when used as donors for nuclear transplantation, produce embryonic stem cells at a higher efficiency than blastocysts derived from terminally differentiated neuronal donor cells, demonstrating a correlation between the state of differentiation and cloning efficiency. Second, using a hypomorphic allele of DNA methyltransferase-1, we found that global hypomethylation of a differentiated cell genome improved cloning efficiency. Our results provide functional evidence that the differentiation and epigenetic state of the donor nucleus influences reprogramming efficiency.
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Citations
Cites
Induction of tumors in mice by genomic hypomethylation
Gaudet, F., Hodgson, J. G., Eden, A., Jackson-Grusby, L., Dausman, J., Gray, J. W., Leonhardt, H., & Jaenisch, R. (2003) · Science · doi:10.1126/science.1083558 · Cited by 1,499 · cited 2×
“Chip/null compound heterozygous mice survive but are runted and develop tumors [25].”
BackgroundSelf-citation
Cited by
Bernstein, B. E., Meissner, A., & Lander, E. S. (2007) · Cell · doi:10.1016/j.cell.2007.01.033 · Cited by 2,086
Self-citationMaherali, N., Sridharan, R., Xie, W., Utikal, J., Eminli, S., Arnold, K., Stadtfeld, M., Yachechko, R., Tchieu, J., Jaenisch, R., et al. (2007) · Cell stem cell · doi:10.1016/j.stem.2007.05.014 · Cited by 1,725
Self-citationLinking the p53 tumour suppressor pathway to somatic cell reprogramming
Kawamura, T., Suzuki, J., Wang, Y. V., Menendez, S., Morera, L. B., Raya, A., Wahl, G. M., & Belmonte, J. C. I. (2009) · Nature · doi:10.1038/nature08311 · Cited by 1,115
Utikal, J., Maherali, N., Kulalert, W., & Hochedlinger, K. (2009) · Journal of Cell Science · doi:10.1242/jcs.054783 · Cited by 346
Progress in understanding reprogramming to the induced pluripotent state
Plath, K., & Lowry, W. E. (2011) · Nature Reviews Genetics · doi:10.1038/nrg2955 · Cited by 289
Metadata
- Authors
- Blelloch, R., Wang, Z., Meissner, A., Pollard, S., Smith, A., & Jaenisch, R.
- Venue
- STEM CELLS
- Publisher
- Oxford University Press (OUP)
- Published
- 2006-09
- Volume
- 24
- Issue
- 9
- Pages
- 2007-2013
- ISSN
- 1066-5099 (ISSN-L)
- 1549-4918
- OpenAlex
- W2067423804
- Cited by
- 277
- References
- 1
- FWCI
- 11.91
- Type
- journal article
- Language
- en
- Open access
- bronze
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