The DOI matches its Crossref record; 43 of the 43 references it cites were found in a registry or an index.
Sox2 is dispensable for the reprogramming of melanocytes and melanoma cells into induced pluripotent stem cells
Open accessAbstract
Induced pluripotent stem cells (iPSCs) have been derived at low frequencies from different cell types through ectopic expression of the transcription factors Oct4 and Sox2, combined with either Klf4 and c-Myc or Lin28 and Nanog. In order to generate iPSCs more effectively, it will be crucial to identify somatic cells that are easily accessible and possibly require fewer factors for conversion into iPSCs. Here, we show that both human and mouse melanocytes give rise to iPSCs at higher efficiencies than fibroblasts. Moreover, we demonstrate that a mouse malignant melanoma cell line, which has previously been reprogrammed into embryonic stem cells by nuclear transfer, remains equally amenable to reprogramming into iPSCs by these transcription factors. In contrast to skin fibroblasts, melanocytes and melanoma cells did not require ectopic Sox2 expression for conversion into iPSCs. iPSC lines from melanocytic cells expressed pluripotency markers, formed teratomas and contributed to viable chimeric mice with germ line transmission. Our results identify skin melanocytes as an alternative source for deriving patient-specific iPSCs at increased efficiency and with fewer genetic elements. In addition, our results suggest that cancer cells remain susceptible to transcription factor-mediated reprogramming, which should facilitate the study of epigenetic changes in human cancer.
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Cites
Takahashi, K., & Yamanaka, S. (2006) · Cell · doi:10.1016/j.cell.2006.07.024 · Cited by 26,673
Induction of Pluripotent Stem Cells from Adult Human Fibroblasts by Defined Factors
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Induced Pluripotent Stem Cell Lines Derived from Human Somatic Cells
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Generation of germline-competent induced pluripotent stem cells
Okita, K., Ichisaka, T., & Yamanaka, S. (2007) · Nature · doi:10.1038/nature05934 · Cited by 4,299
Cre reporter strains produced by targeted insertion of EYFP and ECFP into the ROSA26 locus
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In vitro reprogramming of fibroblasts into a pluripotent ES-cell-like state
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Self-citationReprogramming of human somatic cells to pluripotency with defined factors
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Generation of induced pluripotent stem cells without Myc from mouse and human fibroblasts
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BackgroundSelf-citationGeneration of Mouse Induced Pluripotent Stem Cells Without Viral Vectors
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Cited by
A Small-Molecule Inhibitor of Tgf-β Signaling Replaces Sox2 in Reprogramming by Inducing Nanog
Ichida, J. K., Blanchard, J., Lam, K., Son, E. Y., Chung, J. E., Egli, D., Loh, K. M., Carter, A. C., Di Giorgio, F. P., Koszka, K., et al. (2009) · Cell Stem Cell · doi:10.1016/j.stem.2009.09.012 · Cited by 808
Generation of human induced pluripotent stem cells from urine samples
Zhou, T., Benda, C., Dunzinger, S., Huang, Y., Ho, J. C., Yang, J., Wang, Y., Zhang, Y., Zhuang, Q., Li, Y., et al. (2012) · Nature Protocols · doi:10.1038/nprot.2012.115 · Cited by 614
Progress toward the clinical application of patient-specific pluripotent stem cells
Kiskinis, E., & Eggan, K. (2010) · Journal of Clinical Investigation · doi:10.1172/jci40553 · Cited by 339
SOX2 in development and cancer biology
Novak, D., Hüser, L., Elton, J. J., Umansky, V., Altevogt, P., & Utikal, J. (2020) · Seminars in Cancer Biology · doi:10.1016/j.semcancer.2019.08.007 · Cited by 328
Self-citationAasen, T., & Belmonte, J. C. I. (2010) · Nature Protocols · doi:10.1038/nprot.2009.241 · Cited by 317
Metadata
- Authors
- Utikal, J., Maherali, N., Kulalert, W., & Hochedlinger, K.
- Venue
- Journal of Cell Science
- Publisher
- The Company of Biologists
- Published
- 2009-10-01
- Volume
- 122
- Issue
- 19
- Pages
- 3502-3510
- ISSN
- 0021-9533 (ISSN-L)
- 1477-9137
- OpenAlex
- W2157785989
- Cited by
- 346
- References
- 43
- FWCI
- 13.32
- Type
- journal article
- Language
- en
- Open access
- green
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- Utikal, J., Maherali, N., Kulalert, W., & Hochedlinger, K. (2009). Sox2 is dispensable for the reprogramming of melanocytes and melanoma cells into induced pluripotent stem cells. Journal of Cell Science. https://doi.org/10.1242/jcs.054783
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Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
