The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Derivation, Characterization, and Differentiation of Human Embryonic Stem Cells
Abstract
The derivation of human embryonic stem (hES) cells establishes a new avenue to approach many issues in human biology and medicine for the first time. To meet the increased demand for characterized hES cell lines, we present the derivation and characterization of six hES cell lines. In addition to the previously described immunosurgery procedure, we were able to propagate the inner cell mass and establish hES cell lines from pronase-treated and hatched blastocysts. The cell lines were extensively characterized by expression analysis of markers characteristic for undifferentiated and differentiated hES cells, karyotyping, telomerase activity measurement, and pluripotency assays in vitro and in vivo. Whereas three of the cell lines expressed all the characteristics of undifferentiated pluripotent hES cells, one cell line carried a chromosome 13 trisomy while maintaining an undifferentiated pluripotent state, and two cell lines, one of which carried a triploid karyotype, exhibited limited pluripotency in vivo. Furthermore, we clonally derived one cell line, which could be propagated in an undifferentiated pluripotent state.
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McEachern, M. J., Krauskopf, A., & Blackburn, E. H. (2000) · Annual Review of Genetics · doi:10.1146/annurev.genet.34.1.331 · Cited by 716
Cited by
Characterization and culture of human embryonic stem cells
Hoffman, L. M., & Carpenter, M. K. (2005) · Nature Biotechnology · doi:10.1038/nbt1102 · Cited by 465
Suemori, H., Yasuchika, K., Hasegawa, K., Fujioka, T., Tsuneyoshi, N., & Nakatsuji, N. (2006) · Biochemical and Biophysical Research Communications · doi:10.1016/j.bbrc.2006.04.135 · Cited by 300
Prokhorova, T. A., Harkness, L. M., Frandsen, U., Ditzel, N., Schrøder, H. D., Burns, J. S., & Kassem, M. (2009) · Stem Cells and Development · doi:10.1089/scd.2007.0266 · Cited by 277
Artifactual Insulin Release From Differentiated Embryonic Stem Cells
Hansson, M., Tonning, A., Frandsen, U., Petri, A., Rajagopal, J., Englund, M. C. O., Heller, R. S., Håkansson, J., Fleckner, J., Sköld, H. N., et al. (2004) · Diabetes · doi:10.2337/diabetes.53.10.2603 · Cited by 225
Self-citationZeng, X., & Rao, M. S. (2007) · Neuroscience · doi:10.1016/j.neuroscience.2006.09.017 · Cited by 92
Metadata
- Authors
- Heins, N., Englund, M. C. O., Sjöblom, C., Dahl, U., Tonning, A., Bergh, C., Lindahl, A., Hanson, C., & Semb, H.
- Venue
- STEM CELLS
- Publisher
- Oxford University Press (OUP)
- Published
- 2004-05
- Volume
- 22
- Issue
- 3
- Pages
- 367-376
- ISSN
- 1066-5099 (ISSN-L)
- 1549-4918
- OpenAlex
- W2002508731
- Cited by
- 316
- References
- 1
- FWCI
- 10.28
- Type
- journal article
- Language
- en
- Open access
- closed
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- Heins, N., Englund, M. C. O., Sjöblom, C., Dahl, U., Tonning, A., Bergh, C., Lindahl, A., Hanson, C., & Semb, H. (2004). Derivation, Characterization, and Differentiation of Human Embryonic Stem Cells. STEM CELLS. https://doi.org/10.1634/stemcells.22-3-367
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