The DOI matches its Crossref record; 37 of the 37 references it cites were found in a registry or an index.
Study of Telomere Length Reveals Rapid Aging of Human Marrow Stromal Cells following In Vitro Expansion
Open accessAbstract
Human marrow stromal cells (MSCs) can be isolated from bone marrow and differentiate into multiple tissues in vitro and in vivo. These properties make them promising tools in cell and gene therapy. The lack of a specific MSC marker and the low frequency of MSCs in bone marrow necessitate their isolation by in vitro expansion prior to clinical use. This may severely reduce MSC proliferative capacity to the point that the residual proliferative potential is insufficient to maintain long-term tissue regeneration upon reinfusion. In this study we determined the effect of in vitro expansion on the replicative capacity of MSCs by correlating their rate of telomere loss during in vitro expansion with their behavior in vivo. We report that even protocols that involve minimal expansion induce a rapid aging of MSCs, with losses equivalent to about half their total replicative lifespan.
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1 retracted
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Cited by
Stolzing, A., Jones, E., McGonagle, D., & Scutt, A. (2008) · Mechanisms of Ageing and Development · doi:10.1016/j.mad.2007.12.002 · Cited by 1,195
Lin, H., Sohn, J., Shen, H., Langhans, M. T., & Tuan, R. S. (2019) · Biomaterials · doi:10.1016/j.biomaterials.2018.06.026 · Cited by 445
Bone Marrow Mesenchymal Stem Cells: Historical Overview and Concepts
Charbord, P. (2010) · Human Gene Therapy · doi:10.1089/hum.2010.115 · Cited by 441
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Self-citation
Metadata
- Authors
- Baxter, M. A., Wynn, R. F., Jowitt, S. N., Wraith, J. E., Fairbairn, L. J., & Bellantuono, I.
- Venue
- STEM CELLS
- Publisher
- Oxford University Press (OUP)
- Published
- 2004-09
- Volume
- 22
- Issue
- 5
- Pages
- 675-682
- ISSN
- 1066-5099 (ISSN-L)
- 1549-4918
- OpenAlex
- W1998461585
- Cited by
- 771
- References
- 37
- FWCI
- 7.80
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Baxter, M. A., Wynn, R. F., Jowitt, S. N., Wraith, J. E., Fairbairn, L. J., & Bellantuono, I. (2004). Study of Telomere Length Reveals Rapid Aging of Human Marrow Stromal Cells following In Vitro Expansion. STEM CELLS. https://doi.org/10.1634/stemcells.22-5-675
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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