The DOI matches its Crossref record.
Isolation of a Highly Clonogenic and Multipotential Subfraction of Adult Stem Cells from Bone Marrow Stroma
Abstract
Attempts have been made to develop cell and gene therapies using the adult stem cells from bone marrow referred to as mesenchymal stem cells or marrow stromal cells (MSCs). However, the results have been variable in part because there are no standardized protocols for preparing and characterizing MSCs. In the experiments presented here, we developed a standardized assay by light scattering to measure the content of rapidly self-renewing cells (RS cells) in preparations of MSCs. The assay quickly identifies preparations of MSCs that replicate rapidly in subsequent culture. In addition, the standardized assay enabled us to isolate RS cells that were up to 90% clonogenic and that generated single cell-derived colonies that differentiated into either mineralizing cells or adipocytes with appropriate additions to the medium.
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Cited by
Tögel, F., Weiss, K., Yang, Y., Hu, Z., Zhang, P., & Westenfelder, C. (2007) · American Journal of Physiology-Renal Physiology · doi:10.1152/ajprenal.00339.2006 · Cited by 641
Prockop, D. J. (2009) · Molecular Therapy · doi:10.1038/mt.2009.62 · Cited by 577
Self-citationCardiac Stem Cells and Mechanisms of Myocardial Regeneration
Leri, A., Kajstura, J., & Anversa, P. (2005) · Physiological Reviews · doi:10.1152/physrev.00013.2005 · Cited by 451
Papaccio, F., Paino, F., Regad, T., Papaccio, G., Desiderio, V., & Tirino, V. (2017) · Stem Cells Translational Medicine · doi:10.1002/sctm.17-0138 · Cited by 292
Chen, X., Lin, X., Zhao, J., Shi, W., Zhang, H., Wang, Y., Kan, B., Du, L., Wang, B., Wei, Y., et al. (2008) · Molecular Therapy · doi:10.1038/mt.2008.3 · Cited by 173
Metadata
- Authors
- Smith, J. R., Pochampally, R., Perry, A., Hsu, S., & Prockop, D. J.
- Venue
- STEM CELLS
- Publisher
- Oxford University Press (OUP)
- Published
- 2004-09
- Volume
- 22
- Issue
- 5
- Pages
- 823-831
- ISSN
- 1066-5099 (ISSN-L)
- 1549-4918
- OpenAlex
- W2068888599
- Cited by
- 224
- References
- 0
- FWCI
- 6.10
- Type
- journal article
- Language
- en
- Open access
- closed
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- CoChat. (2026). Citation check: Isolation of a Highly Clonogenic and Multipotential Subfraction of Adult Stem Cells from Bone Marrow Stroma. https://app.cochat.ai/papers/10.1634/stemcells.22-5-823
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- Smith, J. R., Pochampally, R., Perry, A., Hsu, S., & Prockop, D. J. (2004). Isolation of a Highly Clonogenic and Multipotential Subfraction of Adult Stem Cells from Bone Marrow Stroma. STEM CELLS. https://doi.org/10.1634/stemcells.22-5-823
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