Antioxidant dietary supplementation in mice exposed to proton radiation attenuates expression of programmed cell death-associated genes
Open accessAbstract
Dietary antioxidants have radioprotective effects after ionizing radiation exposure that limit hematopoietic cell depletion. We sought to determine the mechanism of proton-induced hematopoietic cell death in animals receiving a moderate dose of whole-body proton radiation. In addition, animals were maintained on diets supplemented with or without dietary antioxidants. In the presence of the dietary antioxidants, total bone marrow mRNA and protein expression of apoptosis-related genes were decreased compared to the expression profiles in the irradiated mice not receiving the antioxidant formulation. These data confirm high-energy proton-induced gene expression of classical apoptosis markers including BAX, caspase-3 and PARP-1. Antioxidant supplementation resulted in decreased expression of these genes in addition to increased protein expression of the anti-apoptosis markers Bcl2 and Bcl-xL. In conclusion, oral supplementation with antioxidants appears to be an effective approach for radioprotection against hematopoietic cell death.
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- Authors
- Sanzari, J. K., Wambi, C., Lewis-Wambi, J. S., & Kennedy, A. R.
- Affiliations
- University of Pennsylvania (US)
- Fox Chase Cancer Center (US)
- Corresponding author
- Kennedy, A. R.
- Venue
- Radiation Research
- Publisher
- Radiation Research Society
- Published
- 2011-05
- Volume
- 175
- Issue
- 5
- Pages
- 650-656
- ISSN
- 0033-7587 (ISSN-L)
- 1938-5404
- PMC
- PMC3382992
- OpenAlex
- W2088377119
- Cited by
- 16
- References
- 34
- Citations by year
- 2023: 1 · 2020: 2 · 2017: 1 · 2016: 2 · 2015: 3 · 2014: 4 · 2013: 1 · 2012: 2
- FWCI
- 1.71
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Radiation Research (publishedVersion)
- PubMed (publishedVersion)
- PubMed Central (acceptedVersion)
- BioOne Complete (BioOne) (submittedVersion)
- Funders
- Rural Development Administration
- National Synchrotron Radiation Laboratory
- National Institutes of Health
- National Cancer Institute (2T32CA00967, K01CA120051, T32CA009677)
- Topics
- Health Sciences › Medicine › Radiology, Nuclear Medicine and Imaging › Effects of Radiation Exposure
- Health Sciences › Medicine › Pulmonary and Respiratory Medicine › Radiation Therapy and Dosimetry
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Cell death mechanisms and regulation
- Keywords
- dietary antioxidants, proton radiation, radiation protection, apoptosis, bone marrow gene expression, Bax, caspase-3, PARP1, Bcl-2, Bcl-xL, whole-body irradiation
- MeSH terms
- Animals, Antioxidants, Bone Marrow Cells, Cell Count, Gene Expression Regulation, Male, Protons, RNA, Messenger, Time Factors, Whole-Body Irradiation, Up-Regulation, Apoptosis, Proto-Oncogene Proteins c-bcl-2, Dietary Supplements, bcl-X Protein, Mice, Transforming Growth Factor beta1
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