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RNAi Knockdown of a Salivary Transcript Leading to Lethality in the Pea Aphid,Acyrthosiphon pisum

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Mutti, Navdeep S., Park, Yoonseong, Reese, John C., Reeck, Gerald R.·2006·Journal of Insect Science

Abstract

Abstract Injection of siRNA (small interfering RNA) into parthenogenetic adult pea aphids (Acyrthosiphon pisum) is shown here to lead to depletion of a target salivary gland transcript. The siRNA was generated from double stranded RNA that covered most of the open reading frame of the transcript, which we have called Coo2. The Coo2 transcript level decreases dramatically over a 3-day period after injection of siRNA. With a lag of 1 to 2 days, the siCoo2-RNA injected insects died, on average 8 days before the death of control insects injected with siRNA for green fluorescent protein. It appears, therefore, that siRNA injections into adults will be a useful tool in studying the roles of individual transcripts in aphid salivary glands and suggests that siCoo2-RNA injections can be a useful positive control in such studies.

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Metadata

Authors
Mutti, N. S., Park, Y., Reese, J. C., & Reeck, G. R.
Venue
Journal of Insect Science
Published
2006-10
Volume
6
Issue
38
Pages
1-7
ISSN
1536-2442 (ISSN-L)
OpenAlex
W2108284976
Cited by
343
References
1
FWCI
2.46
Type
journal article
Language
en
Open access
gold

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CoChat. (2026). Citation check: RNAi Knockdown of a Salivary Transcript Leading to Lethality in the Pea Aphid,Acyrthosiphon pisum. https://app.cochat.ai/papers/10.1673/031.006.3801
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Mutti, N. S., Park, Y., Reese, J. C., & Reeck, G. R. (2006). RNAi Knockdown of a Salivary Transcript Leading to Lethality in the Pea Aphid,Acyrthosiphon pisum. Journal of Insect Science. https://doi.org/10.1673/031.006.3801

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