RADIOIMMUNOASSAY OF OXYTOCIN
Abstract
The evaluation of a radioimmunoassay of oxytocin is described. The method involved careful collection and transportation of blood at 4 °C, acidification of the plasma, extraction with Fuller's earth and radioimmunoassay using antisera raised in rabbits immunized against oxytocin conjugated to bovine serum albumin and 125I-labelled oxytocin. The antisera showed insignificant cross-reaction with a variety of small peptides including vasopressin and vasotocin. The limit of detection of the assay was 2·5 pg with intra-assay and interassay coefficients of variation of 7–15% and 12–18% respectively. Seventy-seven per cent (88 out of 116) of the pregnant women tested had detectable maternal plasma oxytocin. Serial samples of maternal plasma showed a significant increase in oxytocin from the first to the second stage of labour and a significant decrease in the third stage. Oxytocin concentrations in the umbilical arterial plasma were significantly higher in patients in labour. The significance of these findings is discussed.
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By the 1980s, many laboratories had generated radioimmunoassays for oxytocin using independently produced antibodies, and most but not all required extraction.
“By the 1980s, many laboratories had generated radioimmunoassays for oxytocin, using independently produced antibodies 58, 72, 73, 74, 94, 95, 96, 97, 98] and most, but not all 99, 100, required extraction.”
From Measuring Oxytocin and Vasopressin: Bioassays, Immunoassays and Random Numbers (2016) · cites this paper as [94]
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Cited by 83
- Authors
- DAWOOD, M. Y., RAGHAVAN, K. S., & POCIASK, C.
- Venue
- Journal of Endocrinology
- Publisher
- Bioscientifica
- Published
- 1978-02-01
- Volume
- 76
- Issue
- 2
- Pages
- 261-270
- ISSN
- 0022-0795 (ISSN-L)
- 1479-6805
- OpenAlex
- W2057341493
- Cited by
- 83
- References
- 0
- Citations by year
- 2026: 1 · 2021: 1 · 2018: 2 · 2016: 1 · 2015: 1 · 2014: 1 · 2013: 1 · 2012: 1
- FWCI
- 13.20
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Endocrinology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Social Sciences › Psychology › Social Psychology › Neuroendocrine regulation and behavior
- Health Sciences › Medicine › Obstetrics and Gynecology › Maternal and Perinatal Health Interventions
- Health Sciences › Medicine › Public Health, Environmental and Occupational Health › Pregnancy-related medical research
- Keywords
- oxytocin, radioimmunoassay, labour, stages of labor, antisera cross-reactivity, vasopressin, arginine vasotocin, assay sensitivity
- MeSH terms
- Antibody Specificity, Female, Fetal Blood, Humans, Labor, Obstetric, Oxytocin, Pregnancy, Radioimmunoassay
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