Optimization of in vitro and ex vitro regeneration and micromorphological studies of Micrococca mercurialis (L.) Benth
Open accessAbstract
A regeneration protocol has been successfully developed using nodal segments and leaf explants of Micrococca mercurialis (L.) Benth.The surface sterilized leaf explants were induced to proliferative green colored callus on Murashige and Skoog (MS) me dium supplemented with 0.5 mg l -1 of 2,4-dichlorophenoxyacetic acid (2,4-D).Shoots were regenerated from the callus on MS medium sup plemen ted with 0.5 mg l -1 6-benzylaminopurine (BAP).The nodal shoot segments res ponded on MS medium augmented with 1.0 mg l -1 BAP.The shoots were mul ti pliedonMSmediumfortifiedwith0.5mgl-1 each of BAP, kinetin (Kin) and 0.1 mg l -1 α-naphthaleneaceticacid(NAA).Themaximum17.3shootswith8.58 cm average length were resulted on this media combination.The shoots were rooted ex vitro when pulse treated with 100 mg l -1 indole-3 butyric acid (IBA).Theplant letsweresuccessfullyestablishedinthefieldwith96%success rate.Mic ro mo rphological studies were conducted for the successive stages of in vitro, ex vitro and in vivo grown plants to analyze the foliar developmental changes taken place across the environments.It has been concluded that the abnormal mor phophysiology of in vitro plantlets were repaired while acclimatization under thegreenhouseconditionsbeforetransferringtothefield,whichspeedupthe micropropagation process and increase the survival rate of the plantlets.K e y w o r d s : Micrococca mercurialis, regeneration, ex vitro rooting, micromorphological studies, organogenesis Р Е З Ю М Е Манокари М., Шехават М.С.Оптимизация процесса регенерации in vitro и ex vitro и микроморфологические исследования Micrococca mercurialis (L.) Benth.Протокол регенерации был успешно разработан с использованием узловых сегментов и эксплантов листьев Micrococca mercurialis (L.) Benth.Из эксплантов листьев был получен пролиферативный зеленый каллус на среде Murashige и Skoog (MS) с добавлением 0,5 мг/л 2,4-дихлорфеноксиуксусной кислоты (2,4-D).Побеги регенерировали из каллюсанасредеMSсдобавлением0,5мг/л6-бензиламинопурина(BAP).СегментыузловыхпобеговотреагировалинасредуMS,дополненную1мг/л BAP.ПобелибылиразмноженынасредеMS,обогащенной0,5мг/лкинетином(Kin)и0,1мг/лα-нафталинуксуснойкислотой(NAA).Максимальное число побегов -17,3, со средней длиной 8.58 см было получено в этой комбинации сред.Побеги были укоренены ex vitro при периодической обработке100мг/линдол-3маслянойкислотой(IBA).Росткибылипо лучены,коэффициентпрорастения-96%.Микроморфологическиеис следо ванияпроводилисьдляпоследовательныхстадийразвитиярастенийin vitro, ex vitroиin vivoдляанализаизмененийразвитиялистьеввразныхсредах.Сде ланвыводотом,чтоаномальнаяморфофизиологияпро ростковin vit ro былакомпенсированаприакклиматизациивтепличныхусловияхдопе реносавприроднуюсреду,чтоускоряетпроцессмикроразмноженияиувеличи ваетвыживаемостьпроростков.
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When producing Persian walnut in vitro explants, it is crucial to undergo hardening and acclimatization processes due to dysfunctional stomata of walnut in vitro-plantlets, resulting in excessive transpiration and heightened sensitivity to environments with increased evaporative demands and solar radiation.
“When producing Persian walnut in vitro explants, it is crucial to undergo hardening and acclimatization processes. This is due to the dysfunctional stomata of walnut in vitro-plantlets, resulting in excessive transpiration and heightened sensitivity to environments with increased evaporative demands and solar radiation (Chandra et al., 2010; Rathore et al., 2013; Manokari and Shekhawat, 2017).”
From Growth, photosynthetic function, and stomatal characteristics of Persian walnut explants in vitro under different light spectra (2023) · cites this paper as Manokari and Shekhawat, 2017
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Saeedi, S. A., Vahdati, K., Sarikhani, S., Daylami, S. D., Davarzani, M., Gruda, N. S., & Aliniaeifard, S. (2023) · Frontiers in Plant Science · doi:10.3389/fpls.2023.1292045 · Cited by 30 · see citing works
- Authors
- Department of Botany, K. M. C. F. P. S., Manokari, M., Shekhawat, M., & Biotechnology Laboratory, D. O. P. S.
- Affiliations
- Kanchi Mamunivar Centre for Post Graduate Studies (IN)
- Far Eastern Branch of the Russian Academy of Sciences (RU)
- Venue
- Botanica Pacifica
- Published
- 2017
- ISSN
- 2226-4701 (ISSN-L)
- 2410-3713
- OpenAlex
- W2726864922
- Cited by
- 6
- References
- 0
- Citations by year
- 2026: 1 · 2023: 1 · 2021: 1 · 2020: 2 · 2018: 1
- FWCI
- 0.34
- Type
- journal article
- Language
- en
- Open access
- diamond
- doi.org
- Locations
- Botanica Pacifica (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Plant tissue culture and regeneration
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Flowering Plant Growth and Cultivation
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Growth and nutrition in plants
- Keywords
- in vitro regeneration, ex vitro rooting, micropropagation, leaf explants, nodal explants, callus induction, 2,4-D, benzyladenine, kinetin, indole-3-butyric acid, acclimatization, micromorphology, organogenesis, plantlet survival
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