Autofluorescence as a measure of senescence in C. elegans: look to red, not blue or green

Lipofuscin: mechanisms of age-related accumulation and influence on cell function12 1Guest Editor: Rajindar S. Sohal 2This article is part of a series of reviews on “Oxidative Stress and Aging.” The full list of papers may be found on the homepage of the journal.

Brunk, U. T., & Terman, A. (2002) · Free Radical Biology and Medicine · doi:10.1016/s0891-5849(02)00959-0 · Cited by 919 · see citing works

Genetic Analysis of Tissue Aging in <i>Caenorhabditis elegans</i> : A Role for Heat-Shock Factor and Bacterial Proliferation

Garigan, D., Hsu, A.-L., Fraser, A. G., Kamath, R. S., Ahringer, J., & Kenyon, C. (2002) · Genetics · doi:10.1093/genetics/161.3.1101 · Cited by 803 · see citing works

Aging in the nematode Caenorhabditis elegans: Major biological and environmental factors influencing life span

Klass, M. R. (1977) · Mechanisms of Ageing and Development · doi:10.1016/0047-6374(77)90043-4 · Cited by 793 · see citing works

Lipofuscin

Terman, A., & Brunk, U. T. (2004) · The International Journal of Biochemistry &amp; Cell Biology · doi:10.1016/j.biocel.2003.08.009 · Cited by 451 · see citing works

Lipofuscin: Formation, Distribution, and Metabolic Consequences

JUNG, T., BADER, N., & GRUNE, T. (2007) · Annals of the New York Academy of Sciences · doi:10.1196/annals.1404.008 · Cited by 415 · see citing works

Lipofuscin: Mechanisms of formation and increase with age

TERMAN, A., & BRUNK, U. T. (1998) · APMIS · doi:10.1111/j.1699-0463.1998.tb01346.x · Cited by 387 · see citing works

Biochemical basis of lipofuscin, ceroid, and age pigment-like fluorophores

Yin, D. (1996) · Free Radical Biology and Medicine · doi:10.1016/0891-5849(96)00175-x · Cited by 379 · see citing works

Oxidative Stress, Accumulation of Biological 'Garbage', and Aging

Terman, A., & Brunk, U. T. (2006) · Antioxidants and Redox Signaling · doi:10.1089/ars.2006.8.197 · Cited by 363 · see citing works

<i>In vivo</i>spectrofluorimetry reveals endogenous biomarkers that report healthspan and dietary restriction in<i>Caenorhabditis elegans</i>

Gerstbrein, B., Stamatas, G., Kollias, N., & Driscoll, M. (2005) · Aging Cell · doi:10.1111/j.1474-9726.2005.00153.x · Cited by 286 · see citing works

Synchronous Growth and Aging of Caenorhabditis elegans in the Presence of Fluorodeoxyuridine

Mitchell, D. H., Stiles, J. W., Santelli, J. S., & Sanadi, D. R. (1979) · Journal of Gerontology · doi:10.1093/geronj/34.1.28 · Cited by 274 · see citing works

You say lipofuscin, we say ceroid: Defining autofluorescent storage material

Seehafer, S. S., & Pearce, D. A. (2006) · Neurobiology of Aging · doi:10.1016/j.neurobiolaging.2005.12.006 · Cited by 212 · see citing works

The autofluorescent “lipofuscin granules” in the intestinal cells of Caenorhabditis elegans are secondary lysosomes

Clokey, G. V., & Jacobson, L. A. (1986) · Mechanisms of Ageing and Development · doi:10.1016/0047-6374(86)90068-0 · Cited by 201 · see citing works

MicroRNA Predictors of Longevity in Caenorhabditis elegans

Pincus, Z., Smith-Vikos, T., & Slack, F. J. (2011) · PLoS Genetics · doi:10.1371/journal.pgen.1002306 · Cited by 191 · see citing works

The C. elegans spe-9 Gene Encodes a Sperm Transmembrane Protein that Contains EGF-like Repeats and Is Required for Fertilization

Singson, A. W., Mercer, K. B., & L’Hernault, S. W. (1998) · Cell · doi:10.1016/s0092-8674(00)81147-2 · Cited by 157 · see citing works

Molecular Fluorescence Excitation-Emission Matrices Relevant to Tissue Spectroscopy¶

DaCosta, R. S., Andersson, H., & Wilson, B. C. (2003) · Photochemistry and Photobiology · doi:10.1562/0031-8655(2003)0780384mfemrt2.0.co2 · Cited by 154 · see citing works

Lipofuscin: Resolution of Discrepant Fluorescence Data

Eldred, G. E., Miller, G. V., Stark, W. S., & Feeney-Burns, L. (1982) · Science · doi:10.1126/science.7079738 · Cited by 148 · see citing works

Anthranilate Fluorescence Marks a Calcium-Propagated Necrotic Wave That Promotes Organismal Death in C. elegans

Coburn, C., Allman, E. L., Mahanti, P., Benedetto, A., Cabreiro, F., Pincus, Z., Matthijssens, F., Araiz, C., Mandel, A., Vlachos, M., et al. (2013) · PLoS Biology · doi:10.1371/journal.pbio.1001613 · Cited by 143 · see citing works

Production of Age-Synchronous Mass Cultures of Caenorhabditis elegans

Fabian, T. J., & Johnson, T. E. (1994) · Journal of Gerontology · doi:10.1093/geronj/49.4.b145 · Cited by 138 · see citing works

A simple method for maintaining large, aging populations of Caenorhabditis elegans

Gandhi, S., Santelli, J. S., Mitchell, D., Stiles, J. W., & Sanadi, D. R. (1980) · Mechanisms of Ageing and Development · doi:10.1016/0047-6374(80)90090-1 · Cited by 137 · see citing works

Ceroid/lipofuscin formation in cultured human fibroblasts: the role of oxidative stress and lysosomal proteolysis

Terman, A., & Brunk, U. T. (1998) · Mechanisms of Ageing and Development · doi:10.1016/s0047-6374(98)00073-6 · Cited by 132 · see citing works

Variable Pathogenicity Determines Individual Lifespan in Caenorhabditis elegans

Sánchez-Blanco, A., & Kim, S. K. (2011) · PLoS Genetics · doi:10.1371/journal.pgen.1002047 · Cited by 106 · see citing works

Total luminescence spectroscopy of fluorescence changes during aging in Caenorhabditis elegans

Davis, B., Anderson, G. L., & Dusenbery, D. B. (1982) · Biochemistry · doi:10.1021/bi00260a027 · Cited by 67 · see citing works

Manipulation of in vivo iron levels can alter resistance to oxidative stress without affecting ageing in the nematode C. elegans

Valentini, S., Cabreiro, F., Ackerman, D., Alam, M. M., Kunze, M. B. A., Kay, C. W. M., & Gems, D. (2012) · Mechanisms of Ageing and Development · doi:10.1016/j.mad.2012.03.003 · Cited by 64 · see citing works

Quantification of age pigments (lipofuscin)

Hammer, C., & Braum, E. (1988) · Comparative Biochemistry and Physiology Part B Comparative Biochemistry · doi:10.1016/0305-0491(88)90030-2 · Cited by 45 · see citing works

Microfluorometric and fluorometric lipofuscin spectral discrepancies: A concentration-dependent metachromatic effect?

Yin, D., & Brunk, U. T. (1991) · Mechanisms of Ageing and Development · doi:10.1016/0047-6374(91)90076-c · Cited by 31 · see citing works

Modulation of aging profiles in isogenic populations of Caenorhabditis elegans by bacteria causing different extrinsic mortality rates

Baeriswyl, S., Diard, M., Mosser, T., Leroy, M., Manière, X., Taddéi, F., & Matić, I. (2009) · Biogerontology · doi:10.1007/s10522-009-9228-0 · Cited by 30 · see citing works

Nematode autofluorescence and its use as an indicator of viability.

Forge, T. A., & MacGuidwin, A. E. (1989) · PubMed · Cited by 26 · see citing works

Age‐dependent changes and biomarkers of aging in Caenorhabditis elegans

Son, H. G., Altıntaş, Ö., Kim, E. J. E., Kwon, S., & Lee, S. (2019) · Aging Cell · doi:10.1111/acel.12853 · Cited by 185 · see citing works

Caenorhabditis elegans: A Convenient In Vivo Model for Assessing the Impact of Food Bioactive Compounds on Obesity, Aging, and Alzheimer's Disease

Shen, P., Yue, Y., Zheng, J., & Park, Y. (2017) · Annual Review of Food Science and Technology · doi:10.1146/annurev-food-030117-012709 · Cited by 156 · see citing works

New live screening of plant-nematode interactions in the rhizosphere

O’Callaghan, F. E., Braga, R. A., Neilson, R., MacFarlane, S. A., & Dupuy, L. X. (2018) · Scientific Reports · doi:10.1038/s41598-017-18797-7 · Cited by 150 · see citing works

Strong increase in the autofluorescence of cells signals struggle for survival

Surre, J., Saint‐Ruf, C., Collin, V., Orenga, S., Ramjeet, M., & Matić, I. (2018) · Scientific Reports · doi:10.1038/s41598-018-30623-2 · Cited by 142 · see citing works

Healthspan Maintenance and Prevention of Parkinson's-like Phenotypes with Hydroxytyrosol and Oleuropein Aglycone in C. elegans

Brunetti, G., Di Rosa, G., Scuto, M., Leri, M., Stefani, M., Schmitz-Linneweber, C., Calabrese, V., & Saul, N. (2020) · International Journal of Molecular Sciences · doi:10.3390/ijms21072588 · Cited by 138 · see citing works

Lipids as Regulators of Cellular Senescence

Hamsanathan, S., & Gurkar, A. U. (2022) · Frontiers in Physiology · doi:10.3389/fphys.2022.796850 · Cited by 120 · see citing works

Healthspan Enhancement by Olive Polyphenols in C. elegans Wild Type and Parkinson’s Models

Rosa, G. D., Brunetti, G., Scuto, M. C., Salinaro, A. T., Calabrese, E. J., Crea, R., Schmitz‐Linneweber, C., Calabrese, V., & Saul, N. (2020) · International Journal of Molecular Sciences · doi:10.3390/ijms21113893 · Cited by 112 · see citing works

Extended Twilight among Isogenic C. elegans Causes a Disproportionate Scaling between Lifespan and Health

Zhang, W. B., Sinha, D. B., Pittman, W. E., Hvatum, E., Stroustrup, N., & Pincus, Z. (2016) · Cell Systems · doi:10.1016/j.cels.2016.09.003 · Cited by 98 · see citing works

Antioxidant and anti-aging effects of a sea cucumber protein hydrolyzate and bioinformatic characterization of its composing peptides

Guo, K., Su, L., Wang, Y., Liu, H., Lin, J., Peng, C., Yin, X., Liang, M., Wang, Q., & Huang, Z. (2020) · Food & Function · doi:10.1039/d0fo00560f · Cited by 88 · see citing works

Spermidine inhibits neurodegeneration and delays aging via the PINK1-PDR1-dependent mitophagy pathway in C. elegans

Yang, X., Zhang, M., Dai, Y.-H., Sun, Y., Aman, Y., Xu, Y., Yu, P., Zheng, Y., Yang, J. J., & Zhu, X. (2020) · Aging · doi:10.18632/aging.103578 · Cited by 84 · see citing works

Caenorhabditis elegans as an in vivo model for the identification of natural antioxidants with anti-aging actions

Lin, Y., Lin, C., Cao, Y., & Chen, Y. (2023) · Biomedicine & Pharmacotherapy · doi:10.1016/j.biopha.2023.115594 · Cited by 80 · see citing works

Ginsenoside Prolongs the Lifespan of C. elegans via Lipid Metabolism and Activating the Stress Response Signaling Pathway

Yu, X., Li, H., Lin, D., Guo, W., Xu, Z., Wang, L., & Guan, S. (2021) · International Journal of Molecular Sciences · doi:10.3390/ijms22189668 · Cited by 77 · see citing works

Caenorhabditis Elegans and Probiotics Interactions from a Prolongevity Perspective

Roselli, M., Schifano, E., Guantario, B., Zinno, P., Uccelletti, D., & Devirgiliis, C. (2019) · International Journal of Molecular Sciences · doi:10.3390/ijms20205020 · Cited by 72 · see citing works

Overcoming Autofluorescence to Assess GFP Expression During Normal Physiology and Aging in Caenorhabditis elegans

Teuscher, A. C., & Ewald, C. Y. (2018) · BIO-PROTOCOL · doi:10.21769/bioprotoc.2940 · Cited by 67 · see citing works

A parthenogenetic quasi-program causes teratoma-like tumors during aging in wild-type C. elegans

Wang, H., Zhao, Y., Ezcurra, M., Benedetto, A., Gilliat, A. F., Hellberg, J. E. E. U., Ren, Z., Galimov, E. R., Athigapanich, T., Girstmair, J., et al. (2018) · npj Aging and Mechanisms of Disease · doi:10.1038/s41514-018-0025-3 · Cited by 65 · see citing works

Antioxidation and anti-aging activities of astaxanthin geometrical isomers and molecular mechanism involved in Caenorhabditis elegans

Liu, X., Chen, X., Liu, H., & Cao, Y. (2018) · Journal of Functional Foods · doi:10.1016/j.jff.2018.03.004 · Cited by 63 · see citing works

Naringenin prolongs lifespan and delays aging mediated by IIS and MAPK in Caenorhabditis elegans

Ge, Y., Chen, H., Wang, J., Liu, G., Cui, S. W., Kang, J., Jiang, Y., & Wang, H. (2021) · Food & Function · doi:10.1039/d1fo02472h · Cited by 57 · see citing works

Ergothioneine improves healthspan of aged animals by enhancing cGPDH activity through CSE-dependent persulfidation

Petrovic, D., Slade, L., Paikopoulos, Y., D’Andrea, D., Savic, N., Stančić, A., Miljković, J. L., Vignane, T., Drekolia, M. K., Mladenović, D. R., et al. (2025) · Cell Metabolism · doi:10.1016/j.cmet.2024.12.008 · Cited by 54 · see citing works

Caenorhabditis elegans as a Model Host to Monitor the Candida Infection Processes

Elkabti, A. B., Issi, L., & Rao, R. P. (2018) · Journal of Fungi · doi:10.3390/jof4040123 · Cited by 51 · see citing works

Bacopa monnieri (L.) wettst. Extract protects against glutamate toxicity and increases the longevity of Caenorhabditis elegans

Brimson, J. M., Prasanth, M. I., Plaingam, W., & Tencomnao, T. (2020) · Journal of Traditional and Complementary Medicine · doi:10.1016/j.jtcme.2019.10.001 · Cited by 51 · see citing works

Optically Robust and Biocompatible Mechanosensitive Upconverting Nanoparticles

Lay, A., Sheppard, O. H., Siefe, C., McLellan, C. A., Mehlenbacher, R. D., Fischer, S., Goodman, M. B., & Dionne, J. A. (2019) · ACS Central Science · doi:10.1021/acscentsci.9b00300 · Cited by 50 · see citing works

Shatavarin IV elicits lifespan extension and alleviates Parkinsonism in Caenorhabditis elegans

Smita, S. S., Sammi, S. R., Laxman, T. S., Bhatta, R. S., & Pandey, R. (2017) · Free Radical Research · doi:10.1080/10715762.2017.1395419 · Cited by 45 · see citing works

Swimming Exercise and Transient Food Deprivation in Caenorhabditis elegans Promote Mitochondrial Maintenance and Protect Against Chemical-Induced Mitotoxicity

Hartman, J. H., Smith, L. L., Gordon, K. L., Laranjeiro, R., Driscoll, M., Sherwood, D. R., & Meyer, J. N. (2018) · Scientific Reports · doi:10.1038/s41598-018-26552-9 · Cited by 45 · see citing works

Autofluorescence as a noninvasive biomarker of senescence and advanced glycation end products in Caenorhabditis elegans

Komura, T., Yamanaka, M., Nishimura, K., Hara, K., & Nishikawa, Y. (2021) · npj Aging and Mechanisms of Disease · doi:10.1038/s41514-021-00061-y · Cited by 45 · see citing works

Ferulic Acid Supplementation Increases Lifespan and Stress Resistance via Insulin/IGF-1 Signaling Pathway in C. elegans

Li, H., Yu, X., Meng, F., Zhao, Z., Guan, S., & Wang, L. (2021) · International Journal of Molecular Sciences · doi:10.3390/ijms22084279 · Cited by 43 · see citing works

In vivo imaging of fluorescent single-walled carbon nanotubes within C. elegans nematodes in the near-infrared window

Hendler‐Neumark, A., Wulf, V., & Bisker, G. (2021) · Materials Today Bio · doi:10.1016/j.mtbio.2021.100175 · Cited by 43 · see citing works

Curcumin Acetylsalicylate Extends the Lifespan of Caenorhabditis elegans

Zhou, L., Liu, J., Bu, L.-L., Liao, D., Cheng, S., & Zheng, X. (2021) · Molecules · doi:10.3390/molecules26216609 · Cited by 42 · see citing works

The Oxidation-Induced Autofluorescence Hypothesis: Red Edge Excitation and Implications for Metabolic Imaging

Семенов, А. Н., Yakimov, B. P., Rubekina, A. A., Gorin, D. A., Drachev, V. P., Zarubin, M. P., Velikanov, A. N., Lademann, J., Fadeev, V. V., Priezzhev, A. V., et al. (2020) · Molecules · doi:10.3390/molecules25081863 · Cited by 41 · see citing works

Selenium Stimulates Cadmium Detoxification in Caenorhabditis elegans through Thiols-Mediated Nanoparticles Formation and Secretion

Li, L., Cui, Y.-H., Lu, L.-Y., Liu, Y., Zhu, C., Tian, L., Li, W., Zhang, X., Cheng, H., Ma, J., et al. (2019) · Environmental Science & Technology · doi:10.1021/acs.est.8b04200 · Cited by 41 · see citing works

Autofluorescence of yeast Saccharomyces cerevisiae cells caused by glucose metabolism products and its methodological implications

Maślanka, R., Kwolek‐Mirek, M., & Zadrąg‐Tęcza, R. (2018) · Journal of Microbiological Methods · doi:10.1016/j.mimet.2018.01.017 · Cited by 40 · see citing works

The role of Caenorhabditis elegans in the discovery of natural products for healthy aging

Kirchweger, B., Zwirchmayr, J., Grienke, U., & Rollinger, J. M. (2023) · Natural Product Reports · doi:10.1039/d3np00021d · Cited by 40 · see citing works

Coupling of Rigor Mortis and Intestinal Necrosis during C. elegans Organismal Death

Galimov, E. R., Pryor, R. E., Poole, S. E., Benedetto, A., Pincus, Z., & Gems, D. (2018) · Cell Reports · doi:10.1016/j.celrep.2018.02.050 · Cited by 35 · see citing works

Molecular Nanomachines Can Destroy Tissue or Kill Multicellular Eukaryotes

Gunasekera, R. S., Galbadage, T., Ayala‐Orozco, C., Liu, D., García‐López, V., Troutman, B. E., Tour, J. J., Pál, R., Krishnan, S., Cirillo, J. D., et al. (2020) · ACS Applied Materials & Interfaces · doi:10.1021/acsami.9b22595 · Cited by 32 · see citing works

Enhanced Healthspan in Caenorhabditis elegans Treated With Extracts From the Traditional Chinese Medicine Plants Cuscuta chinensis Lam. and Eucommia ulmoides Oliv.

Sayed, S. M. A., Siems, K., Schmitz‐Linneweber, C., Luyten, W. H. M. L., & Saul, N. (2021) · Frontiers in Pharmacology · doi:10.3389/fphar.2021.604435 · Cited by 32 · see citing works

Vitis Vinifera Leaf Extract Protects Against Glutamate-Induced Oxidative Toxicity in HT22 Hippocampal Neuronal Cells and Increases Stress Resistance Properties in Caenorhabditis Elegans

Duangjan, C., Rangsinth, P., Zhang, S., Gu, X., Wink, M., & Tencomnao, T. (2021) · Frontiers in Nutrition · doi:10.3389/fnut.2021.634100 · Cited by 28 · see citing works

Global, cell non-autonomous gene regulation drives individual lifespan among isogenic C. elegans

Kinser, H. E., Mosley, M. C., Plutzer, I. B., & Pincus, Z. (2021) · eLife · doi:10.7554/elife.65026 · Cited by 27 · see citing works

All-Optical Wide-Field Selective Imaging of Fluorescent Nanodiamonds in Cells, In Vivo and Ex Vivo

Yanagi, T., Kaminaga, K., Suzuki, M., Abe, H., Yamamoto, H., Ohshima, T., Kuwahata, A., Sekino, M., Imaoka, T., Kakinuma, S., et al. (2021) · ACS Nano · doi:10.1021/acsnano.0c07740 · Cited by 26 · see citing works

Artemisia selengensis Turcz. leaf extract promotes longevity and stress resistance in Caenorhabditis elegans

Li, R., Tao, M., Xu, T., Huang, Y., Zogona, D., Pan, S., Wu, T., & Xu, X. (2022) · Journal of the Science of Food and Agriculture · doi:10.1002/jsfa.11808 · Cited by 26 · see citing works

Genistein Promotes Anti-Heat Stress and Antioxidant Effects via the Coordinated Regulation of IIS, HSP, MAPK, DR, and Mitochondrial Pathways in Caenorhabditis elegans

Zhang, S., Qin, Z.-C., Sun, Y.-Y., Chen, Y., Chen, W., Wang, H., An, D., Sun, D., & Liu, Y. (2023) · Antioxidants · doi:10.3390/antiox12010125 · Cited by 25 · see citing works

SKN‐1/NRF2 upregulation by vitamin A is conserved from nematodes to mammals and is critical for lifespan extension in Caenorhabditis elegans

Sirakawin, C., Lin, D., Zhou, Z., Wang, X., Kelleher, R., Huang, S., Long, W., Pires‐daSilva, A., Liu, Y., Wang, J., et al. (2023) · Aging Cell · doi:10.1111/acel.14064 · Cited by 25 · see citing works

The rice bran peptide KF-8 extends the lifespan and improves the healthspan of Caenorhabditis elegans via skn-1 and daf-16

Cai, J., Chen, Z., Wu, Y., Chen, Y., Wang, J., Lin, Q., & Liang, Y. (2022) · Food & Function · doi:10.1039/d1fo03718h · Cited by 25 · see citing works

The Endogenous Metabolite Glycerophosphocholine Promotes Longevity and Fitness in Caenorhabditis elegans

Liu, J., Zheng, R.-Q., Wang, Y., Liu, Y., Jiang, S., Wang, X., He, K., Pan, X., Zhou, T., Li, T., et al. (2022) · Metabolites · doi:10.3390/metabo12020177 · Cited by 24 · see citing works

Lysosome-related organelles promote stress and immune responses in C. elegans

Hajdú, G., Somogyvári, M., Csermely, P., & Sőti, C. (2023) · Communications Biology · doi:10.1038/s42003-023-05246-7 · Cited by 24 · see citing works

A simple culture system for long-term imaging of individual C. elegans

Pittman, W. E., Sinha, D. B., Zhang, W. B., Kinser, H. E., & Pincus, Z. (2017) · Lab on a Chip · doi:10.1039/c7lc00916j · Cited by 24 · see citing works

Health and longevity studies in C. elegans: the “healthy worm database” reveals strengths, weaknesses and gaps of test compound-based studies

Saul, N., Möller, S., Cirulli, F., Berry, A., Luyten, W. H. M. L., & Fuellen, G. (2021) · Biogerontology · doi:10.1007/s10522-021-09913-2 · Cited by 22 · see citing works

Redox-mediated regulation of aging and healthspan by an evolutionarily conserved transcription factor HLH-2/Tcf3/E2A

Rozanov, L. F., Ravichandran, M., Grigolon, G., Zanellati, M. C., Mansfeld, J., Zarse, K., Barzilai, N., Atzmon, G., Fischer, F., & Ristow, M. (2020) · Redox Biology · doi:10.1016/j.redox.2020.101448 · Cited by 21 · see citing works

Mechanical Insights into Thiol-Mediated Synergetic Biotransformation of Cadmium and Selenium in Nematodes

Li, F., Wu, Q.-Z., Chen, J., Cui, Y.-H., Zhu, C., Li, B., Guo, Z. G., & Li, W. (2021) · Environmental Science & Technology · doi:10.1021/acs.est.1c00686 · Cited by 23 · see citing works

Red Cabbage Rather Than Green Cabbage Increases Stress Resistance and Extends the Lifespan of Caenorhabditis elegans

Zhang, N., Jiao, S., & Jing, P. (2021) · Antioxidants · doi:10.3390/antiox10060930 · Cited by 22 · see citing works

Volumetric imaging of fast cellular dynamics with deep learning enhanced bioluminescence microscopy

Morales-Curiel, L.-F., Gonzalez, A. C., Castro‐Olvera, G., Lin, L.-C., El‐Quessny, M., Porta-de-la-Riva, M., Severino, J., Batlle‐Morera, L., Venturini, V., Ruprecht, V., et al. (2022) · Communications Biology · doi:10.1038/s42003-022-04292-x · Cited by 23 · see citing works

Healthspan Improvements in Caenorhabditis elegans with Traditional Chinese Herbal Tea

Lin, C., Zhang, X., Zhuang, C., Lin, Y., Cao, Y., & Chen, Y. (2020) · Oxidative Medicine and Cellular Longevity · doi:10.1155/2020/4057841 · Cited by 22 · see citing works

Rebaudioside A Enhances Resistance to Oxidative Stress and Extends Lifespan and Healthspan in Caenorhabditis elegans

Li, P., Wang, Z., Lam, S. M., & Shui, G. (2021) · Antioxidants · doi:10.3390/antiox10020262 · Cited by 22 · see citing works

piRNAs regulate a Hedgehog germline-to-soma pro-aging signal

Shi, C., & Murphy, C. T. (2023) · Nature Aging · doi:10.1038/s43587-022-00329-2 · Cited by 21 · see citing works

Ratiometric Nanothermometer Based on a Radical Excimer for In Vivo Sensing

Blasi, D., Gonzalez‐Pato, N., Rodríguez, X. R., Diez‐Zabala, I., Srinivasan, S. Y., Camarero, N., Esquivias, O., Roldán, M., Guasch, J., Laromaine, A., et al. (2023) · Small · doi:10.1002/smll.202207806 · Cited by 21 · see citing works

Noncanonical inheritance of phenotypic information by protein amyloids

Eroglu, M., Zocher, T., McAuley, J., Webster, R., Xiao, M. Z. X., Yu, B., Mok, C. A., & Derry, W. B. (2024) · Nature Cell Biology · doi:10.1038/s41556-024-01494-9 · Cited by 26 · see citing works

Celeganser: Automated Analysis of Nematode Morphology and Age

Wang, L., Kong, S., Pincus, Z., & Fowlkes, C. C. (2020) · IEEE/CVF Conference on Computer Vision and Pattern Recognition Workshops (CVPRW) · doi:10.1109/cvprw50498.2020.00492 · Cited by 20 · see citing works

Use of Flavin-Related Cellular Autofluorescence to Monitor Processes in Microbial Biotechnology

Müllerová, L., Markova, K., Obruča, S., & Mravec, F. (2022) · Microorganisms · doi:10.3390/microorganisms10061179 · Cited by 19 · see citing works

An In Vivo Microfluidic Study of Bacterial Load Dynamics and Absorption in the C. elegans Intestine

Viri, V., Arveiler, M., Lehnert, T., & Gijs, M. A. M. (2021) · Micromachines · doi:10.3390/mi12070832 · Cited by 19 · see citing works

Machine learning-based analysis of microfluidic device immobilized C. elegans for automated developmental toxicity testing

DuPlissis, A., Medewar, A., Hegarty, E., Laing, A., Shen, A., Gomez, S., Mondal, S., & Ben‐Yakar, A. (2025) · Scientific Reports · doi:10.1038/s41598-024-84842-x · Cited by 20 · see citing works

Imaging metals in Caenorhabditis elegans

Aschner, M., Palinski, C., Sperling, M., Kärst, U., Schwerdtle, T., & Bornhorst, J. (2016) · Metallomics · doi:10.1039/c6mt00265j · Cited by 18 · see citing works

CemOrange2 fusions facilitate multifluorophore subcellular imaging in C. elegans

Thomas, B. J., Wight, I. E., Chou, W. Y. Y., Moreno, M., Dawson, Z. D., Homayouni, A. L., Huang, H., Kim, H., Jia, H., Buland, J. R., et al. (2019) · PLoS ONE · doi:10.1371/journal.pone.0214257 · Cited by 18 · see citing works

Saponin‐rich extracts fromHolothuria leucospilotamediate lifespan extension and stress resistance inCaenorhabditis elegansviadaf‐16

Kitisin, T., Suphamungmee, W., & Meemon, K. (2019) · Journal of Food Biochemistry · doi:10.1111/jfbc.13075 · Cited by 18 · see citing works

The Attenuation of Insulin/IGF-1 Signaling Pathway Plays a Crucial Role in the Myo-Inositol-Alleviated Aging in Caenorhabditis elegans

Yang, N.-C., Chin, C.-Y., Zheng, Y., & Lee, I. (2023) · International Journal of Molecular Sciences · doi:10.3390/ijms24076194 · Cited by 17 · see citing works

Determination of health status during aging using bending and pumping rates at various survival rates in Caenorhabditis elegans

Cho, J., Lu, J., Kim, D., & Park, Y. (2025) · Scientific Reports · doi:10.1038/s41598-025-93876-8 · Cited by 15 · see citing works

SAIBR: a simple, platform-independent method for spectral autofluorescence correction

Rodrigues, N. T. L., Bland, T., Borrego-Pinto, J., Ng, K., Hirani, N., Gu, Y., Foo, S., & Goehring, N. W. (2022) · Development · doi:10.1242/dev.200545 · Cited by 17 · see citing works

Measuring and modeling interventions in aging

Stroustrup, N. (2018) · Current Opinion in Cell Biology · doi:10.1016/j.ceb.2018.07.004 · Cited by 17 · see citing works

High-speed large-scale 4D activities mapping of moving C. elegans by deep-learning-enabled light-field microscopy on a chip

Zhu, T., Zhu, L., Li, Y., Chen, X., He, M., Li, G., Zhang, H., Gao, S., & Fei, P. (2021) · Sensors and Actuators B Chemical · doi:10.1016/j.snb.2021.130638 · Cited by 16 · see citing works

Visualizing and quantifying molecular and cellular processes in Caenorhabditis elegans using light microscopy

Shah, P. K., Bao, Z., & Zaidel‐Bar, R. (2022) · Genetics · doi:10.1093/genetics/iyac068 · Cited by 17 · see citing works

A new utilization of total flavonoids from Acer truncatum samara and leaves: Anti-aging and metabolic regulation

Zhang, X., Wang, D.-S., Li, X., Miao, Y.-C., Gao, J., & Zhang, Q. (2023) · Industrial Crops and Products · doi:10.1016/j.indcrop.2023.117207 · Cited by 16 · see citing works

The million-molecule challenge: a moonshot project to rapidly advance longevity intervention discovery

Lee, M. B., Blue, B. W., Muir, M., & Kaeberlein, M. R. (2023) · GeroScience · doi:10.1007/s11357-023-00867-6 · Cited by 15 · see citing works

Phosphorothioate-DNA bacterial diet reduces the ROS levels in C. elegans while improving locomotion and longevity

Huang, Q., Li, R., Yi, T., Cong, F., Wang, D., Deng, Z., & Zhao, Y. (2021) · Communications Biology · doi:10.1038/s42003-021-02863-y · Cited by 15 · see citing works

Structural characterization and anti-aging activity investigation of a polysaccharide from Anemarrhena asphodeloides Bge

Fan, X., Yu, L., Xie, Y., Yan, X., Fan, W., Ishii, Y., Yu, B., & Li, R. (2024) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2024.139216 · Cited by 14 · see citing works

Behavioral and molecular markers of death in Drosophila melanogaster

Tower, J., Agrawal, S., Alagappan, M. P., Bell, H. S., Demeter, M., Havanoor, N., Hegde, V. S., Jia, Y., Kothawade, S. N., Lin, X., et al. (2019) · Experimental Gerontology · doi:10.1016/j.exger.2019.110707 · Cited by 15 · see citing works

The Lifespan Extension Ability of Nicotinic Acid Depends on Whether the Intracellular NAD+ Level Is Lower than the Sirtuin-Saturating Concentrations

Yang, N.-C., Cho, Y.-H., & Lee, I. (2019) · International Journal of Molecular Sciences · doi:10.3390/ijms21010142 · Cited by 14 · see citing works

Synthesis, anti-aging and mechanism of magnolol derivatives

Pang, X., Mao, L., Ye, D., Wang, W., Yang, H., Fan, X., Yang, Y., Su, Z., Ma, T., Sun, M., et al. (2023) · Frontiers in Chemistry · doi:10.3389/fchem.2023.1180375 · Cited by 13 · see citing works

Markers and mechanisms of death in Drosophila

Tower, J. (2023) · Frontiers in Aging · doi:10.3389/fragi.2023.1292040 · Cited by 13 · see citing works

Measurement of the membrane elasticity of red blood cell with osmotic pressure by optical tweezers.

Wu, J., Li, Y., Lu, D., Liu, Z., Cheng, Z., & He, L. (2009) · PubMed · Cited by 13 · see citing works

Planococcus maritimu ML1206 Strain Enhances Stress Resistance and Extends the Lifespan in Caenorhabditis elegans via FOXO/DAF-16

Wu, J.-S., Lin, C.-G., Jin, C., Zhou, Y., & Li, Y.-X. (2022) · Marine Drugs · doi:10.3390/md21010001 · Cited by 12 · see citing works

Direct observation of fluorescent proteins in gels: A rapid, cost‐efficient, and quantitative alternative to immunoblotting

Sanial, M., Miled, R., Alves, M., Claret, S. B., Joly, N., Proux‐Gillardeaux, V., Plessis, A., & Sébastien Léon, A. (2025) · Biology of the Cell · doi:10.1111/boc.202400161 · Cited by 9 · see citing works

Cordycepin extends the longevity of Caenorhabditis elegans via antioxidation and regulation of fatty acid metabolism

Sun, Y., Zhong, M., Wang, J., Feng, M., Shen, C., Han, Z., Cao, X., & Zhang, Q. (2025) · European Journal of Pharmacology · doi:10.1016/j.ejphar.2025.177388 · Cited by 11 · see citing works

Spatiotemporal Tracking of Near‐Infrared Fluorescent Single‐Walled Carbon Nanotubes in C. Elegans Nematodes Confined in a Microfluidics Platform

Sharaga, E., Hendler‐Neumark, A., Kamber, D., & Bisker, G. (2023) · Advanced Materials Technologies · doi:10.1002/admt.202301222 · Cited by 11 · see citing works

Pathogen-induced dormancy in liquid limits gastrointestinal colonization of Caenorhabditis elegans

Zhang, L., Gade, V., & Kirienko, N. V. (2023) · Virulence · doi:10.1080/21505594.2023.2204004 · Cited by 10 · see citing works

Application of zein nanoparticles for the treatment of inflammatory bowel diseases

Gagliardi, A., Valle, M. D., Espuelas, S., Irache, J. M., & Cosco, D. (2025) · Expert Opinion on Drug Delivery · doi:10.1080/17425247.2025.2527693 · Cited by 10 · see citing works

Fluorescent toys ‘n’ tools lighting the way in fungal research

Genechten, W. V., Dijck, P. V., & Demuyser, L. (2021) · FEMS Microbiology Reviews · doi:10.1093/femsre/fuab013 · Cited by 10 · see citing works

Intrinsic properties of anisakid nematode larvae as a potential tool for the detection in fish products

Klapper, R., Karl, H., & Ostermeyer, U. (2021) · International Journal of Food Microbiology · doi:10.1016/j.ijfoodmicro.2021.109094 · Cited by 9 · see citing works

Antioxidant and Antiaging Activity of Houttuynia cordata Thunb. Ethyl Acetate Fraction in Caenorhabditis elegans

Kim, H., Mun, J.-S., Oh, S., & Kim, J.-H. (2024) · Nutrients · doi:10.3390/nu16234168 · Cited by 9 · see citing works

Toxic Effects of Domoic Acid on Caenorhabditis elegans and the Underlying Mechanism

Tian, D., & Zhang, G. (2019) · International Journal of Biology · doi:10.5539/ijb.v11n3p1 · Cited by 9 · see citing works

Early life long-term exposure to aflatoxin B1 induces aging and alters innate immunity associated with SKN-1/Nrf2 in Caenorhabditis elegans

Chang, T.-T., Chang, C., & Liao, V. H. (2024) · Chemico-Biological Interactions · doi:10.1016/j.cbi.2024.111349 · Cited by 8 · see citing works

Similarities and differences in the gene expression signatures of physiological age versus future lifespan

Mosley, M. C., Kinser, H. E., Martin, O. M. F., Stroustrup, N., Schedl, T., Kornfeld, K., & Pincus, Z. (2024) · Aging Cell · doi:10.1111/acel.14428 · Cited by 7 · see citing works

Identifying C. elegans lifespan mutants by screening for early-onset protein aggregation

Midkiff, D., Huayta, J., Lichty, J. D., Crapster, J. P., & San‐Miguel, A. (2022) · iScience · doi:10.1016/j.isci.2022.105460 · Cited by 7 · see citing works

Dual-species proteomics and targeted intervention of animal-pathogen interactions

Liu, Y. S., Zhang, C., Khoo, B. L., Hao, P., & Chua, S. L. (2024) · Journal of Advanced Research · doi:10.1016/j.jare.2024.08.038 · Cited by 7 · see citing works

A BRET Ca 2+ sensor enables high-throughput screening in the presence of background fluorescence

Cumberbatch, D., Mori, T., Yang, J., Mi, D., Vinson, P. N., Weaver, C. D., & Johnson, C. H. (2022) · Science Signaling · doi:10.1126/scisignal.abq7618 · Cited by 7 · see citing works

Lycium barbarum Polysaccharide Alleviates Amyloid‐β‐Induced Toxicity in the Caenorhabditis elegans Model

An, L., Wang, W., Chu, J., An, L., Geng, B., Fu, X., Chen, J., & Ma, J. (2024) · Food Frontiers · doi:10.1002/fft2.522 · Cited by 6 · see citing works

Biomarkers for aging in Caenorhabditis elegans high throughput screening

Yarmey, V. R., & San‐Miguel, A. (2024) · Biochemical Society Transactions · doi:10.1042/bst20231303 · Cited by 6 · see citing works

Evaluating stress resilience of cyanobacteria through flow cytometry and fluorescent viability assessment

Kroupová, Z., Slaninová, E., Mrázová, K., Krzyžánek, V., Hrubanová, K., Fritz, I., & Obruča, S. (2024) · Folia Microbiologica · doi:10.1007/s12223-024-01212-w · Cited by 6 · see citing works

Potato Cyst Nematode Egg Viability Assessment and Preparasitic Juvenile Screening Using a Large Particle Flow Cytometer and Sorter

Pillai, S. S., & Dandurand, L. (2020) · Phytopathology · doi:10.1094/phyto-06-20-0255-r · Cited by 6 · see citing works

High-resolution imaging and analysis of the intestinal bacterial load of Caenorhabditis elegans during early adulthood

Rezaeianaran, F., & Gijs, M. A. M. (2023) · RSC Advances · doi:10.1039/d3ra02934d · Cited by 6 · see citing works

Streblus asper Lour. exerts MAPK and SKN-1 mediated anti-aging, anti-photoaging activities and imparts neuroprotection by ameliorating Aβ in Caenorhabditis elegans

Prasanth, M. I., Brimson, J. M., Malar, D. S., Prasansuklab, A., & Tencomnao, T. (2021) · Nutrition and Healthy Aging · doi:10.3233/nha-210121 · Cited by 6 · see citing works

Ziziphus jujuba polysaccharide degradation products exert neuroprotective and anti-aging effects via modulating gut microbiota and metabolomics in C. elegans

Zhou, C., Bian, L., Zhang, Y., Huang, X., Jia, D., & Ju, Z. (2026) · Journal of Functional Foods · doi:10.1016/j.jff.2026.107213 · Cited by 4 · see citing works

Zein nanoparticles extend lifespan in C. elegans and SAMP8 mice

Martínez‐López, A. L., Reboredo, C., González-Navarro, C. J., Solas, M., Puerta, E., Ramírez, M. J., Vizmanos, J. L., & Irache, J. M. (2024) · International Journal of Pharmaceutics · doi:10.1016/j.ijpharm.2024.124798 · Cited by 4 · see citing works

Toxicological Analysis of the Arylnaphthalene Lignan Justicidin B Using a Caenorhabditis elegans Model

Sciandrone, B., Kentsop, R. A. D., Pensotti, R., Ottolina, G., Mascheretti, I., Mattana, M., & Regonesi, M. E. (2024) · Molecules · doi:10.3390/molecules29235516 · Cited by 4 · see citing works

The biology of death in Caenorhabditis elegans

Coburn, C. (2012) · UCL Discovery (University College London) · Cited by 1 · see citing works

<i>In vivo</i>spectrofluorimetry reveals endogenous biomarkers that report healthspan and dietary restriction in<i>Caenorhabditis elegans</i>

Gerstbrein, B., Stamatas, G., Kollias, N., & Driscoll, M. (2005) · Aging Cell · doi:10.1111/j.1474-9726.2005.00153.x · Cited by 286 · see citing works

Overcoming Autofluorescence to Assess GFP Expression During Normal Physiology and Aging in Caenorhabditis elegans

Teuscher, A. C., & Ewald, C. Y. (2018) · BIO-PROTOCOL · doi:10.21769/bioprotoc.2940 · Cited by 67 · see citing works

Spectral scanning and fluorescence lifetime imaging microscopy (FLIM) enable separation and characterization of C. elegans autofluorescence in the cuticle and gut

Hulsey-Vincent, H., Cameron, E. A., Dahlberg, C. L., & Galati, D. F. (2024) · Biology Open · doi:10.1242/bio.060613 · Cited by 3 · see citing works

Aging related autofluorescence can be used as a marker of cell senescence

Xiao-chun, Z. J. Y. L.-M. C. (2014) · Chieh P'ou Hsueh Pao · doi:10.3969/j.issn.0529-1356.2014.01.001