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Cited by 30melanin and skin pigmentationSkin Protection and AgingMelanoma and MAPK Pathways

Protective effect of madecassoside on H2O2-induced oxidative stress and autophagy activation in human melanocytes

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Ling, Yuting, Gong, Qingli, Xiong, Xixi, Sun, Li, Zhao, Wene, Zhu, Wenyuan, Lu, Yan·2017·Oncotarget
DOI 10.18632/oncotarget.17654PMC PMC5584231
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Abstract

// Yuting Ling 1 , Qingli Gong 1 , Xixi Xiong 1 , Li Sun 1 , Wene Zhao 2 , Wenyuan Zhu 1 and Yan Lu 1 1 Department of Dermatology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu Province, 210029, P.R. China 2 Department of Analysis and Testing Center, Nanjing Medical University, Nanjing, Jiangsu Province, 210029, P.R. China Correspondence to: Yan Lu, email: luyan6289@163.com Keywords: madecassoside, oxidative stress, autophagy, mitochondria, vitiligo Received: December 28, 2016 Accepted: April 19, 2017 Published: May 07, 2017 ABSTRACT Background: Centella asiatica (L.) Urb. is a traditional Chinese medicine that has many medical applications, including wound healing and anti-oxidation. Some traditional Chinese Medicine doctors have found that it has therapeutic effects for external use in the repigmentation of vitiligo and post-inflammatory hypopigmentation. This study was designed to evaluate the effects of madecassoside, a major bioactive component of C. asiatica , on oxidative stress in human melanocytes and its possible mechanism of action. Results: In H2O2-induced oxidative conditions, madecassoside inhibited melanocyte dendrite retraction, improved MMP and reduced the accumulation of [Ca2+]i in a concentration-dependent manner. Observations by TEM showed that madecassoside attenuated the damage of mitochondria in human melanocytes caused by oxidative stress. Furthermore, autophagy activation was demonstrated by AO staining and an increased LC3-II/LC3-I ratio. Materials and Methods: Normal human melanocytes were treated with 0.01 mM H2O2 and varying concentrations of madecassoside (0, 10, 50, 100 μg/mL). Subsequently, the retraction velocity of melanocyte dendrites was assessed. Determination of mitochondrial membrane potential (MMP, ΔΨm) was performed by flow cytometry and intracellular calcium ([Ca 2+ ]i) level were measured. Alterations of mitochondrial ultrastructure were observed by transmission electron microscopy (TEM). Acridine orange (AO) staining was used to measure autophagy. The LC3-II/LC3-I ratio, an indicator of autophagosome formation, was analyzed by western blot. Conclusions: These results demonstrate the antioxidative effect of madecassoside on human melanocytes subjected to oxidative damage via the activation of autophagy. Moreover, madecassoside could be a promising treatment for vitiligo mainly caused by oxidative stress.

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  • Madecassoside protects the mitochondrial structure against ROS overproduction and attenuates overall oxidative damage in human melanocytes via activation of autophagy.

    “An experimental study to test the effects of C. asiatica on oxidative stress in human melanocytes demonstrated that madecassoside can protect the mitochondrial structure against ROS overproduction and attenuate the overall oxidative damage in melanocytes via the activation of autophagy. 84”

    From Therapeutic Properties and Pharmacological Activities of Asiaticoside and Madecassoside: A Review (2023) · cites this paper as [84]

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Authors
Ling, Y., Gong, Q., Xiong, X., Sun, L., Zhao, W., Zhu, W., & Lu, Y.
Affiliations
  • Jiangsu Province Hospital (CN)
  • Nanjing Medical University (CN)
  • Department of Dermatology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu Province, 210029, P.R. China
ORCID
Venue
Oncotarget
Publisher
Impact Journals, LLC
Published
2017-05-07
Volume
8
Issue
31
Pages
51066-51075
ISSN
1949-2553 (ISSN-L)
DOI
10.18632/oncotarget.17654
PMC
PMC5584231
OpenAlex
W2612068259
Cited by
30
References
1
Citations by year
2026: 2 · 2025: 2 · 2024: 4 · 2023: 5 · 2022: 3 · 2021: 6 · 2020: 3 · 2019: 2 · 2018: 3
FWCI
1.14
Type
journal article
Language
en
Open access
Licence
cc-by
Locations
  • Oncotarget (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
Funders
  • National Natural Science Foundation of China (81171517)
  • Government of Jiangsu Province
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › melanin and skin pigmentation
  • Health Sciences › Medicine › Dermatology › Skin Protection and Aging
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Melanoma and MAPK Pathways
Keywords
madecassoside, Centella asiatica, oxidative stress, autophagy, human melanocytes, hydrogen peroxide, vitiligo, mitochondrial membrane potential, intracellular calcium, mitochondrial ultrastructure, LC3-II/LC3-I ratio, repigmentation, post-inflammatory hypopigmentation, neuroprotection, neurodegenerative diseases

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