The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Sir2-domain associated short prokaryotic Argonautes provide defence against invading mobile genetic elements through NAD+ depletion
Open accessPreprint — published as 10.1038/s41564-022-01239-0
Abstract
Argonaute (Ago) proteins are found in all three domains of life. The so-called long Agos are composed of four major domains (N, PAZ, MID, and PIWI) and contribute to RNA silencing in eukaryotes (eAgos) or defence against invading mobile genetic elements in prokaryotes (pAgos). Intriguingly, the majority (~60%) of prokaryotic Agos (pAgos) identified bioinformatically are shorter (comprised of only MID and PIWI domains) and are typically associated with Sir2, Mrr or TIR domain-containing proteins. The cellular function and mechanism of short pAgos remain enigmatic. Here, we show that short pAgos from Geobacter sulfurreducens, Caballeronia cordobensis and Paraburkholderia graminis, together with the NAD+-bound Sir2-proteins form a stable heterodimeric Sir2/Ago complex that recognizes invading plasmid or phage DNA through the pAgos subunit and activates Sir2 subunit triggering the endogenous NAD+ depletion and cell death thus preventing the propagation of invading DNA. This is the first demonstration that short Sir2-associated pAgos provide defence against phages and plasmids and underscores the diversity of mechanisms of prokaryotic Agos.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Highly accurate protein structure prediction with AlphaFold
Jumper, J., Evans, R., Pritzel, A., Green, T., Figurnov, M., Ronneberger, O., Tunyasuvunakool, K., Bates, R., Žídek, A., Potapenko, A., et al. (2021) · Nature · doi:10.1038/s41586-021-03819-2 · Cited by 43,764
Flagged first, then by citations
Metadata
- Authors
- Zaremba, M., Dakineviciene, D., Golovinas, E., Stankunas, E., Lopatina, A., Sorek, R., Manakova, E., Ruksenaite, A., Silanskas, A., Ašmontas, S., Grigaitis, R., Timinskas, K., Venclovas, Č., & Siksnys, V.
- Affiliations
- Vilnius University (LT)
- University of Vienna (AT)
- Weizmann Institute of Science (IL)
- Vilnius university
- Vilnius University
- Corresponding author
- Zaremba, Mindaugas
- ORCID
- Venue
- Nature Microbiology
- Published
- 2022-01-06
- Volume
- 7
- Issue
- 11
- Pages
- 1857-1869
- ISSN
- 2058-5276 (ISSN-L)
- OpenAlex
- W4300861916
- Cited by
- 126
- References
- 1
- Citations by year
- 2026: 20 · 2025: 29 · 2024: 31 · 2023: 38 · 2022: 8
- FWCI
- 8.65
- Type
- journal article
- Language
- en
- Open access
- green
- doi.org
- Licence
- Locations
- Nature Microbiology (publishedVersion)
- bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by-nc-nd)
- Research Square (acceptedVersion, cc-by)
- PubMed (publishedVersion)
- Desy Publications Database (Deutsches Elektronen-Synchrotron DESY) (submittedVersion)
- DESY Publication Database (PUBDB) (Deutsches Elektronen-Synchrotron)
- Funders
- Deutsche Forschungsgemeinschaft (464312965, SPP2330)
- Israel Science Foundation (ISF 296/21, SPP 2330)
- Lietuvos Mokslo Taryba (09.3.3-LMT-K-712-01-0126, 09.3.3‐LMT‐K‐712, 3-LMT-K-712)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › CRISPR and Genetic Engineering
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Life Sciences › Immunology and Microbiology › Virology › Bacteriophages and microbial interactions
- Keywords
- Sir2, Geobacter sulfurreducens, NAD+ depletion, NADase activity, phage defense, abortive infection, heterodimeric complex, mobile genetic elements, RNA silencing, cell death
- MeSH terms
- Bacteriophages, DNA, NAD, Prokaryotic Cells, Interspersed Repetitive Sequences, Argonaute Proteins
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
