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PreprintCited by 5Wheat and Barley Genetics and PathologyPlant-Microbe Interactions and ImmunityPlant Disease Resistance and Genetics

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Divergent molecular pathways govern temperature-dependent wheat stem rust resistance genes Sr6, Sr13 and Sr21

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Zhang, Peng, Hewitt, Tim, Sharma, Keshav, Zhang, Jianping, Bajgain, Prabin, Bhatt, Dhara, Yang, Jun, Singh, Smriti, Wang, Qiaoli, Upadhyaya, Narayana, Pozniak, Curtis, McIntosh, Robert, Lagudah, Evans, Rouse, Matthew·2024·Nature Communications
DOI 10.21203/rs.3.rs-4674841/v1PMC PMC12119863

Preprint — published as 10.1038/s41467-025-60030-x

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Abstract

The wheat stem rust pathogen, Puccinia graminis f. sp. tritici (Pgt), has caused devastating crop losses worldwide. Several stem rust resistance (Sr) genes display temperature-dependent immune responses. Sr6-mediated resistance is enhanced at lower temperatures whereas Sr13 and Sr21 resistances are enhanced at higher temperatures. Here we report cloning of Sr6 by mutagenesis and resistance gene enrichment and sequencing (MutRenSeq), showing it to encode an NLR protein with an integrated BED domain. Sr6 temperature sensitivity was also transferred to wheat plants transformed with the Sr6 transgene. Differential gene expression analysis using near-isogenic wheat lines inoculated with Pgt at varying temperatures revealed that genes upregulated in the low-temperature-effective Sr6 response differed significantly from those upregulated in the high-temperature-effective responses associated with Sr13 and Sr21. Understanding the molecular mechanisms and pathways involved in temperature sensitivity can inform future strategies for deployment and engineering of genetic resistance in response to a changing climate.

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Metadata

Authors
Zhang, P., Hewitt, T., Sharma, K., Zhang, J., Bajgain, P., Bhatt, D., Yang, J., Singh, S., Wang, Q., Upadhyaya, N., Pozniak, C., McIntosh, R., Lagudah, E., & Rouse, M.
Affiliations
  • The University of Sydney (AU)
  • Australian National University (AU)
  • Agricultural Research Service (US)
  • University of Minnesota (US)
  • Commonwealth Scientific and Industrial Research Organisation (AU)
  • Henan Agricultural University (CN)
  • University of Saskatchewan (CA)
Corresponding author
  • Zhang, Peng
  • McIntosh, Robert
  • Lagudah, Evans
  • Rouse, Matthew
ORCID
Venue
Nature Communications
Publisher
Springer Science and Business Media LLC
Published
2024-07-16
Volume
16
Issue
1
Pages
4905-4905
ISSN
2041-1723 (ISSN-L)
DOI
10.21203/rs.3.rs-4674841/v1
PMC
PMC12119863
OpenAlex
W4410831546
Cited by
5
References
1
Citations by year
2026: 4 · 2025: 1
FWCI
2.67
Type
journal article
Language
en
Open access
Licence
Locations
  • Nature Communications (publishedVersion, cc-by)
  • Research Square (acceptedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • RePEc: Research Papers in Economics (submittedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
  • U.S. Department of Agriculture
  • Commonwealth Scientific and Industrial Research Organisation
  • Grains Research and Development Corporation
  • Department of Education and Training (IE230100282)
  • Agricultural Research Service
  • Australian Research Council (IE230100282)
Topics
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Wheat and Barley Genetics and Pathology
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant-Microbe Interactions and Immunity
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Disease Resistance and Genetics
Keywords
wheat stem rust, Puccinia graminis f. sp. tritici, stem rust resistance genes, NLR proteins, resistance gene cloning, near-isogenic lines, differential gene expression, plant-pathogen interaction
MeSH terms
NLR Proteins, Puccinia, Basidiomycota, Plant Diseases, Plant Proteins, Temperature, Triticum, Genes, Plant, Gene Expression Regulation, Plant, Plant Stems, Disease Resistance

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