Test of an Ascorbic Acid Method for Determining Phosphorus in Water and NaHCO 3 Extracts from Soil
Abstract
Murphy and Riley proposed use of a single reagent for determining P. The method was found to be accurate for determining P in soil extracts. This method is based on reduction of the ammonium molybdiphosphate complex by ascorbic acid in the presence of antimony. The color produced is stable for 24 hours. It is less subject to interfering substances than are methods involving reduction by SnCl 2 .
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“Following incubation, the cultures were centrifuged at 12,000 g for 15 min and the supernatant was collected to quantify P solubilisation by the molybdenum blue method (Watanabe and Olsen, 1965).”
- Authors
- Watanabe, F. S., & Olsen, S. R.
- Affiliations
- Agricultural Research Service (US)
- ORCID
- Watanabe, F. S.
- Venue
- Soil Science Society of America Journal
- Publisher
- Wiley
- Published
- 1965-11
- Volume
- 29
- Issue
- 6
- Pages
- 677-678
- ISSN
- 0361-5995 (ISSN-L)
- 1435-0661
- OpenAlex
- W1990768879
- Cited by
- 2,485
- References
- 0
- Citations by year
- 2026: 87 · 2025: 87 · 2024: 113 · 2023: 120 · 2022: 139 · 2021: 133 · 2020: 126 · 2019: 117 · 2018: 101 · 2017: 74 · 2016: 91 · 2015: 77 · 2014: 80 · 2013: 89 · 2012: 68
- FWCI
- 2.68
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Soil Science Society of America Journal (publishedVersion)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Soil Science › Plant Growth and Agriculture Techniques
- Physical Sciences › Environmental Science › Water Science and Technology › Environmental and Analytical Chemistry Studies
- Physical Sciences › Environmental Science › Waste Management and Disposal › Phosphorus and nutrient management
- Keywords
- phosphorus determination, ascorbic acid reduction, ammonium molybdophosphate, antimony, soil extracts, aqueous extract, colorimetric assay
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