The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.
Method for Isolation of PCR-Ready Genomic DNA from Zebrafish Tissues
Open accessAbstract
Here we describe a method for the isolation of PCR-ready genomic DNA from various zebrafish tissues that is based on a previously published murine protocol. The DNA solutions are of sufficient quality to allow PCR detection of transgenes from all commonly used zebrafish tissues. In sperm, transgene amplification was successful even when diluted 1000-fold, allowing easy identification of transgenic founders. Given its speed and low cost, we anticipate that the adoption of this method will streamline DNA isolation for zebrafish research.
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Cites
Preparation of PCR-Quality Mouse Genomic DNA with Hot Sodium Hydroxide and Tris (HotSHOT)
Truett, G. E., Heeger, P., Mynatt, R. L., Truett, A. A., Walker, J. A., & Warman, M. L. (2000) · BioTechniques · doi:10.2144/00291bm09 · Cited by 1,738
In vivo tracking of T cell development, ablation, and engraftment in transgenic zebrafish
Langenau, D. M., Ferrando, A. A., Traver, D., Kutok, J. L., Hezel, J.-P. D., Kanki, J. P., Zon, L. I., Look, A. T., & Trede, N. S. (2004) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0402248101 · Cited by 429
Self-citation
Cited by
CRISPRscan: designing highly efficient sgRNAs for CRISPR-Cas9 targeting in vivo
Moreno-Mateos, M. A., Vejnar, C. E., Beaudoin, J.-D., Fernandez, J. P., Mis, E. K., Khokha, M. K., & Giraldez, A. J. (2015) · Nature Methods · doi:10.1038/nmeth.3543 · Cited by 1,290
In vivo genome editing using a high-efficiency TALEN system
Bedell, V. M., Wang, Y., Campbell, J. M., Poshusta, T. L., Starker, C. G., Krug II, R. G., Tan, W., Penheiter, S. G., Ma, A. C., Leung, A. Y. H., et al. (2012) · Nature · doi:10.1038/nature11537 · Cited by 914
Gagnon, J. A., Valen, E., Thyme, S. B., Huang, P., Ahkmetova, L., Pauli, A., Montague, T. G., Zimmerman, S., Richter, C., & Schier, A. F. (2014) · PLoS ONE · doi:10.1371/journal.pone.0098186 · Cited by 889
Whole-organism lineage tracing by combinatorial and cumulative genome editing
McKenna, A., Findlay, G. M., Gagnon, J. A., Horwitz, M. S., Schier, A. F., & Shendure, J. (2016) · Science · doi:10.1126/science.aaf7907 · Cited by 766
Rees, H. A., Komor, A. C., Yeh, W.-H., Caetano-Lopes, J., Warman, M., Edge, A. S. B., & Liu, D. R. (2017) · Nature Communications · doi:10.1038/ncomms15790 · Cited by 412
Metadata
- Authors
- Meeker, N. D., Hutchinson, S. A., Ho, L., & Trede, N. S.
- Venue
- BioTechniques
- Publisher
- Informa UK Limited
- Published
- 2007-11
- Volume
- 43
- Issue
- 5
- Pages
- 610-614
- ISSN
- 0736-6205 (ISSN-L)
- 1940-9818
- OpenAlex
- W2155117797
- Cited by
- 517
- References
- 2
- FWCI
- 1.74
- Type
- journal article
- Language
- en
- Open access
- gold
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- Meeker, N. D., Hutchinson, S. A., Ho, L., & Trede, N. S. (2007). Method for Isolation of PCR-Ready Genomic DNA from Zebrafish Tissues. BioTechniques. https://doi.org/10.2144/000112619
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