The DOI matches its Crossref record; 27 of the 27 references it cites were found in a registry or an index.
In vivo genome editing using a high-efficiency TALEN system
Open accessAbstract
The zebrafish (Danio rerio) is increasingly being used to study basic vertebrate biology and human disease with a rich array of in vivo genetic and molecular tools. However, the inability to readily modify the genome in a targeted fashion has been a bottleneck in the field. Here we show that improvements in artificial transcription activator-like effector nucleases (TALENs) provide a powerful new approach for targeted zebrafish genome editing and functional genomic applications. Using the GoldyTALEN modified scaffold and zebrafish delivery system, we show that this enhanced TALEN toolkit has a high efficiency in inducing locus-specific DNA breaks in somatic and germline tissues. At some loci, this efficacy approaches 100%, including biallelic conversion in somatic tissues that mimics phenotypes seen using morpholino-based targeted gene knockdowns. With this updated TALEN system, we successfully used single-stranded DNA oligonucleotides to precisely modify sequences at predefined locations in the zebrafish genome through homology-directed repair, including the introduction of a custom-designed EcoRV site and a modified loxP (mloxP) sequence into somatic tissue in vivo. We further show successful germline transmission of both EcoRV and mloxP engineered chromosomes. This combined approach offers the potential to model genetic variation as well as to generate targeted conditional alleles.
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Cites
Effective targeted gene ‘knockdown’ in zebrafish
Nasevicius, A., & Ekker, S. C. (2000) · Nature Genetics · doi:10.1038/79951 · Cited by 2,483
Self-citationBreaking the Code of DNA Binding Specificity of TAL-Type III Effectors
Boch, J., Scholze, H., Schornack, S., Landgraf, A., Hahn, S., Kay, S., Lahaye, T., Nickstadt, A., & Bonas, U. (2009) · Science · doi:10.1126/science.1178811 · Cited by 2,470
In Vivo Imaging of Embryonic Vascular Development Using Transgenic Zebrafish
Lawson, N. D., & Weinstein, B. M. (2002) · Developmental Biology · doi:10.1006/dbio.2002.0711 · Cited by 2,151
A TALE nuclease architecture for efficient genome editing
Miller, J. C., Tan, S., Qiao, G., Barlow, K. A., Wang, J., Xia, D. F., Meng, X., Paschon, D. E., Leung, E., Hinkley, S. J., et al. (2011) · Nature Biotechnology · doi:10.1038/nbt.1755 · Cited by 2,098
A Simple Cipher Governs DNA Recognition by TAL Effectors
Moscou, M. J., & Bogdanove, A. J. (2009) · Science · doi:10.1126/science.1178817 · Cited by 1,988
Cermak, T., Doyle, E. L., Christian, M., Wang, L., Zhang, Y., Schmidt, C., Baller, J. A., Somia, N. V., Bogdanove, A. J., & Voytas, D. F. (2011) · Nucleic Acids Research · doi:10.1093/nar/gkr218 · Cited by 1,787
Self-citationHeritable targeted gene disruption in zebrafish using designed zinc-finger nucleases
Doyon, Y., McCammon, J. M., Miller, J. C., Faraji, F., Ngo, C., Katibah, G. E., Amora, R., Hocking, T. D., Zhang, L., Rebar, E. J., et al. (2008) · Nature Biotechnology · doi:10.1038/nbt1409 · Cited by 896
Transplantation and in vivo imaging of multilineage engraftment in zebrafish bloodless mutants
Traver, D., Paw, B. H., Poss, K. D., Penberthy, W. T., Lin, S., & Zon, L. I. (2003) · Nature Immunology · doi:10.1038/ni1007 · Cited by 800
Targeted gene inactivation in zebrafish using engineered zinc-finger nucleases
Meng, X., Noyes, M. B., Zhu, L. J., Lawson, N. D., & Wolfe, S. A. (2008) · Nature Biotechnology · doi:10.1038/nbt1398 · Cited by 741
A novel TALE nuclease scaffold enables high genome editing activity in combination with low toxicity
Mussolino, C., Morbitzer, R., Lütge, F., Dannemann, N., Lahaye, T., & Cathomen, T. (2011) · Nucleic Acids Research · doi:10.1093/nar/gkr597 · Cited by 714
Cited by
ZFN, TALEN, and CRISPR/Cas-based methods for genome engineering
Gaj, T., Gersbach, C. A., & Barbas, C. F. (2013) · Trends in Biotechnology · doi:10.1016/j.tibtech.2013.04.004 · Cited by 3,820
TALENs: A widely applicable technology for targeted genome editing
Joung, J. K., & Sander, J. D. (2013) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm3486 · Cited by 1,650
“As with ZFNs, TALENs have enabled efficient introduction of targeted alterations in a number of model organisms that were previously difficult or impossible to manipulate genetically such as fruit fly 6 , roundworm 7 , zebrafish 9–11 , frog 12 , rat 13 , and pig 14 .”
Redesigning photosynthesis to sustainably meet global food and bioenergy demand
Ort, D. R., Merchant, S. S., Alric, J., Barkan, A., Blankenship, R. E., Bock, R., Croce, R., Hanson, M. R., Hibberd, J. M., Long, S. P., et al. (2015) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1424031112 · Cited by 1,020
Highly efficient CRISPR/Cas9-mediated knock-in in zebrafish by homology-independent DNA repair
Auer, T. O., Duroure, K., De Cian, A., Concordet, J.-P., & Del Bene, F. (2014) · Genome Research · doi:10.1101/gr.161638.113 · Cited by 631
Genome Engineering with Targetable Nucleases
Carroll, D. (2014) · Annual Review of Biochemistry · doi:10.1146/annurev-biochem-060713-035418 · Cited by 568
Metadata
- Authors
- Bedell, V. M., Wang, Y., Campbell, J. M., Poshusta, T. L., Starker, C. G., Krug II, R. G., Tan, W., Penheiter, S. G., Ma, A. C., Leung, A. Y. H., Fahrenkrug, S. C., Carlson, D. F., Voytas, D. F., Clark, K. J., Essner, J. J., & Ekker, S. C.
- Venue
- Nature
- Published
- 2012-09-23
- Volume
- 491
- Issue
- 7422
- Pages
- 114-118
- ISSN
- 0028-0836 (ISSN-L)
- 1476-4687
- OpenAlex
- W1999068651
- Cited by
- 914
- References
- 27
- FWCI
- 50.07
- Type
- journal article
- Language
- en
- Open access
- green
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- Bedell, V. M., Wang, Y., Campbell, J. M., Poshusta, T. L., Starker, C. G., Krug II, R. G., Tan, W., Penheiter, S. G., Ma, A. C., Leung, A. Y. H., Fahrenkrug, S. C., Carlson, D. F., Voytas, D. F., Clark, K. J., Essner, J. J., & Ekker, S. C. (2012). In vivo genome editing using a high-efficiency TALEN system. Nature. https://doi.org/10.1038/nature11537
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