The DOI matches its Crossref record.
Gene Splicing by Overlap Extension: Tailor-Made Genes Using the Polymerase Chain Reaction
Open accessAbstract
Gene Splicing by Overlap Extension or "gene SOEing" is a PCR-based method of recombining DNA sequences without reliance on restriction sites and of directly generating mutated DNA fragments in vitro. By modifying the sequences incorporated into the 5'-ends of the primers, any pair of polymerase chain reaction products can be made to share a common sequence at one end. Under polymerase chain reaction conditions, the common sequence allows strands from two different fragments to hybridize to one another, forming an overlap. Extension of this overlap by DNA polymerase yields a recombinant molecule. This powerful and technically simple approach offers many advantages over conventional approaches for manipulating gene sequences.
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Cited by
Corr, S. C., Li, Y., Riedel, C. U., O'Toole, P. W., Hill, C., & Gahan, C. G. M. (2007) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0700440104 · Cited by 853
A nuclear localization domain in the hnRNP A1 protein.
Siomi, H., & Dreyfuss, G. (1995) · The Journal of cell biology · doi:10.1083/jcb.129.3.551 · Cited by 503
Protein-tyrosine-phosphatase SHPTP2 couples platelet-derived growth factor receptor beta to Ras.
Bennett, A. M., Tang, T. L., Sugimoto, S., Walsh, C. T., & Neel, B. G. (1994) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.91.15.7335 · Cited by 397
Boehm, J., Kang, M.-G., Johnson, R. C., Esteban, J., Huganir, R. L., & Malinow, R. (2006) · Neuron · doi:10.1016/j.neuron.2006.06.013 · Cited by 382
Wallner, M., Meera, P., & Toro, L. (1999) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.96.7.4137 · Cited by 373
Metadata
- Authors
- Horton, R. M., Cai, Z., Ho, S. N., & Pease, L. R.
- Venue
- BioTechniques
- Publisher
- Informa UK Limited
- Published
- 2013-03
- Volume
- 54
- Issue
- 3
- Pages
- 129-133
- ISSN
- 0736-6205 (ISSN-L)
- 1940-9818
- OpenAlex
- W1546157935
- Cited by
- 1,289
- References
- 0
- FWCI
- 23.53
- Type
- journal article
- Language
- en
- Open access
- gold
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- Horton, R. M., Cai, Z., Ho, S. N., & Pease, L. R. (2013). Gene Splicing by Overlap Extension: Tailor-Made Genes Using the Polymerase Chain Reaction. BioTechniques. https://doi.org/10.2144/000114017
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
