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GFP and β-Galactosidase Transformation Vectors for Promoter/Enhancer Analysis in Drosophila

Open access
Barolo, Scott, Carver, Lucy A., Posakony, James W.·2000·BioTechniques

Abstract

the efficiency of transfection.In light of the fact that ECs are difficult to transfect, the success of this method is warranted for a wider range of cells with different origins.In fact, we have obtained similar results in temporally transfecting NIH 3T3 cells using this method (data not shown).In addition, this method of temporal transfection might have utility in reversing cellular abnormalities through genetic intervention of the temporally introduced gene.

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Metadata

Authors
Barolo, S., Carver, L. A., & Posakony, J. W.
Venue
BioTechniques
Published
2000-10
Volume
29
Issue
4
Pages
726-732
ISSN
  • 0736-6205 (ISSN-L)
  • 1940-9818
OpenAlex
W947309
Cited by
346
References
0
FWCI
1.24
Type
journal article
Language
en
Open access
gold

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CoChat. (2026). Citation check: GFP and β-Galactosidase Transformation Vectors for Promoter/Enhancer Analysis in Drosophila. https://app.cochat.ai/papers/10.2144/00294bm10
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Barolo, S., Carver, L. A., & Posakony, J. W. (2000). GFP and β-Galactosidase Transformation Vectors for Promoter/Enhancer Analysis in Drosophila. BioTechniques. https://doi.org/10.2144/00294bm10

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Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.