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Cited by 37Antimicrobial Resistance in StaphylococcusInfection Control in HealthcareInfections and bacterial resistance

A genomic infection control study for Staphylococcus aureus in two Ghanaian hospitals

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Donkor, Eric S., Jamrozy, Dorota, Mills, Richael Odarkor, Dankwah, Thomas, Amoo, Philip, Egyir, Beverly, Badoe, Ebenezer, Twasam, Joana +1 more·2018·Infection and Drug Resistance
DOI 10.2147/idr.s167639PMC PMC6188180
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Abstract

Background: Whole genome sequencing analysis (WGSA) provides the best resolution for typing of bacterial isolates and has the potential for identification of transmission pathways. The aim of the study was to apply WGSA to elucidate the possible transmission events involved in two suspected Staphylococcus aureus hospital outbreaks in Ghana and describe genomic features of the S. aureus isolates sampled in the outbreaks. Methods: The study was carried out at Korle-Bu Teaching Hospital and Lekma Hospital where the suspected outbreaks occurred in 2012 and 2015, respectively. The S. aureus isolates collected from the two hospitals were from three sources including carriage, invasive disease, and the environment. Whole genome sequencing of the S. aureus isolates was performed and the sequence reads were mapped to the S. aureus reference genome of strain USA300_FPR3757. A maximum-likelihood phylogenetic tree was reconstructed. Multilocus sequence typing together with the analysis of antimicrobial resistance and virulence genes were performed by short read mapping using the SRST2. Results: The S. aureus isolates belonged to diverse sequence types (STs) with ST15 and ST152 most common. All isolates carried the blaZ gene, with low prevalence of tetK and dfrG genes also observed. All isolates were mecA negative. The pvl genes were common and observed in distinct lineages that revealed diverse Sa2int phages. At Korle-Bu Teaching Hospital, the genomics data indicated several transmission events of S. aureus ST15 involving contamination of various surfaces in the pediatric emergency ward where the outbreak occurred. Conclusion: The pattern of dissemination of the ST15 clone in the emergency ward of Korle-Bu Teaching Hospital highlights a basic problem with disinfection of environmental surfaces at the hospital. Diverse phage population rather than a single highly transmissible phage type likely mediates the high prevalence of pvl genes among the S. aureus isolates. Keywords: Staphylococcus aureus , sequencing, transmission, outbreak, Ghana

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  • The most prevalent MRSA genotypes in SSA have been demonstrated to be ST5, ST8, ST15, ST88, ST152, ST241, and ST239.

    “Moreover, the most prevalent genotypes have been demonstrated to be ST5, ST8, ST15, ST88, ST152, ST241, and ST239.162,[169][170][171]”

    From Antimicrobial Resistance Rates and Surveillance in Sub-Saharan Africa: Where Are We Now? (2022) · cites this paper as [169]

    The Results (Genomic investigations; page not provided) identify ST15 and ST152 as the most common sequence types among 17 S. aureus isolates from two Ghanaian hospitals, and also detect ST5. The Results (Antimicrobial resistance genes; page not provided) state that all isolates were mecA-negative. The paper therefore does not establish the listed genotypes as the most prevalent MRSA genotypes across sub-Saharan Africa; it does not report ST8, ST88, or ST241 among its isolates. (LLM, conf=0.90)

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Cited by

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Authors
Donkor, E. S., Jamrozy, D., Mills, R. O., Dankwah, T., Amoo, P., Egyir, B., Badoe, E., Twasam, J., & Bentley, S.
Affiliations
  • University of Ghana (GH)
  • Wellcome Sanger Institute (GB)
  • University of Cape Coast (GH)
  • Korle Bu Teaching Hospital (GH)
Corresponding author
Bentley, Stephen
ORCID
Venue
Infection and Drug Resistance
Publisher
Informa UK Limited
Published
2018-10
Volume
Volume 11
Pages
1757-1765
ISSN
1178-6973 (ISSN-L)
DOI
10.2147/idr.s167639
PMC
PMC6188180
OpenAlex
W2599490823
Cited by
37
References
2
Citations by year
2025: 1 · 2024: 9 · 2023: 3 · 2022: 10 · 2021: 5 · 2020: 2 · 2019: 6 · 2018: 1
FWCI
2.65
Type
journal article
Language
en
Open access
Licence
Locations
  • Infection and Drug Resistance (publishedVersion, cc-by-nc)
  • Europe PMC (PubMed Central) (publishedVersion, cc-by-nc)
  • PubMed Central (publishedVersion, cc-by-nc)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
  • Dove Medical Press (Taylor and Francis Group) (submittedVersion, cc-by-nc)
Funders
Wellcome Trust (098051)
Topics
  • Health Sciences › Medicine › Infectious Diseases › Antimicrobial Resistance in Staphylococcus
  • Health Sciences › Medicine › Infectious Diseases › Infection Control in Healthcare
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Endocrinology › Infections and bacterial resistance
Keywords
Staphylococcus aureus, hospital outbreak, infection control, whole genome sequencing, ST15, ST152, Panton-Valentine leukocidin, environmental surface contamination, disinfection, multidrug resistance, virulence genes, phylogenetic analysis

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