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Cited by 25Antibiotic Resistance in BacteriaSalmonella and Campylobacter epidemiologyAntibiotic Use and Resistance

Multidrug-Resistant Gram-Negative Bacteria Contaminating Raw Meat Sold in Accra, Ghana

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Baah, Deric A., Kotey, Fleischer C. N., Dayie, Nicholas T. K. D., Codjoe, Francis S., Tetteh-Quarcoo, Patience B., Donkor, Eric S.·2022·Pathogens
DOI 10.3390/pathogens11121517PMC PMC9784824
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Abstract

Background: Efforts to combat antimicrobial resistance (AMR) should be based on the One Health approach, involving human health, animal health, and the environment. In Ghana, previous studies on AMR have given little attention to animal source food, a major route of transmission of antibiotic-resistant zoonotic pathogens. The aim of this study was to investigate the occurrence of multidrug-resistant (MDR) bacteria in meat sold in Accra. Methods: This was a cross-sectional study in which 270 meat samples (90 each of beef, goat meat, and chicken) were collected, and investigated for contamination with multidrug-resistant bacteria. The bacteria were subjected to susceptibility testing against amikacin (30 µg), ampicillin (10 µg), amoxicillin-clavulanate (20/10 µg), cefuroxime (30 µg), ceftriaxone (30 µg), ceftazidime (30 µg), cefepime (30 µg), ciprofloxacin (5 µg), trimethoprim-sulfamethoxazole (1.25/23.75 µg), ertapenem (10 µg), meropenem (10 µg), imipenem (10 µg), tigecycline (15 µg), and gentamicin (10 µg). Results: Thirty-two different types of bacteria, totalling 558, were isolated, the predominant being Escherichia coli (44.6%), Aeromonas hydrophila (19.9%), Vibrio cholerae (3.4%), Aeromonas veronii (3.2%), and Klebsiella pneumoniae (3.1%). The prevalence of MDR among the contaminating bacteria was 14.9%. The MDR distribution among the predominant bacteria was Escherichia coli (18.7%), Aeromonas hydrophila (11.1%), Vibrio cholerae and Aeromonas veronii (0.0% each), and K. pneumoniae (5.6%). Moreover, 2.0% of the contaminating bacteria were extended-spectrum beta-lactamase (ESBL) producers, all of which occurred in the chicken samples, and their distribution was: Escherichia coli (1.3%), Klebsiella pneumoniae, Pantoea spp., Enterobacter cloacae, and Serratia plymuthica (0.2% each). Conclusions: The meat samples were heavily contaminated with Escherichia coli and Aeromonas hydrophila, and less frequently, with Vibrio cholerae, Klebsiella pneumoniae, and other organisms. The prevalence of multidrug-resistant bacteria was moderate (14.9%), while that of ESBL producers was low (2%).

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  • Six animal studies reported MDR prevalence ranging from 14.9% to 100% in E. coli.

    “Six “animal studies” reported MDR prevalence ranging from 14.9% to 100% in E. coli [45,46,48,49,51,63].”

    From A Systematic Review on Antimicrobial Resistance in Ghana from a One Health Perspective (2024) · cites this paper as [63]

    The Results (§3.2) report 14.9% MDR prevalence among all contaminating bacteria, but 18.7% among E. coli isolates. This paper therefore supports an animal-source meat study reporting MDR in E. coli, but not the attribution of the 14.9% lower bound to E. coli. (LLM, conf=0.88)

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  • Studies have revealed the presence of MDR bacteria, including methicillin-resistant S. aureus (MRSA), ESBL-producing Enterobacteriaceae, and MDR-Salmonella, in meat products.

    “Studies have revealed the presence of MDR bacteria, including methicillin-resistant S. aureus (MRSA), ESBL-producing Enterobacteriaceae, and MDR-Salmonella, in meat products [63,76,77,78,79].”

    From A Systematic Review on Antimicrobial Resistance in Ghana from a One Health Perspective (2024) · cites this paper as [63]

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1 of the 54 references couldn’t be matched to a known paper.

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  1. [40]Çakır E. Ozon Gazı Uygulanmış Koyun Sütü Örneklerinin Fizikokimyasal ve Mikrobiyolojik Özelliklerinde Meydana Gelen Değişimlerin Belirlenmesi Master’s Thesis Necmettin Erbakan University Konya, Turkey 2018

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Authors
Baah, D. A., Kotey, F. C. N., Dayie, N. T. K. D., Codjoe, F. S., Tetteh-Quarcoo, P. B., & Donkor, E. S.
Affiliations
  • University of Ghana (GH)
  • Department of Medical Microbiology, University of Ghana Medical School, Accra P.O. Box KB 4236, Ghana
Corresponding author
Donkor, Eric S.
ORCID
Venue
Pathogens
Publisher
MDPI AG
Published
2022-12-11
Volume
11
Issue
12
Pages
1517
ISSN
2076-0817 (ISSN-L)
DOI
10.3390/pathogens11121517
PMC
PMC9784824
OpenAlex
W4312123967
Cited by
25
References
54
Citations by year
2026: 7 · 2025: 5 · 2024: 7 · 2023: 6
FWCI
1.47
Type
journal article
Language
en
Open access
Licence
Locations
  • Pathogens (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Medicine › Antibiotic Resistance in Bacteria
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Salmonella and Campylobacter epidemiology
  • Life Sciences › Immunology and Microbiology › Applied Microbiology and Biotechnology › Antibiotic Use and Resistance
Keywords
multidrug-resistant bacteria, antimicrobial resistance, raw meat contamination, foodborne pathogens, Escherichia coli, Aeromonas hydrophila, Aeromonas veronii, Vibrio cholerae, Klebsiella pneumoniae, extended-spectrum beta-lactamase, poultry meat, zoonotic transmission, One Health, Accra, Ghana

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