Substrates coated with silver nanoparticles as a neuronal regenerative material.
Open accessAbstract
Much effort has been devoted to the design of effective biomaterials for nerve regeneration. Here, we report the novel use of silver nanoparticles (AgNPs) as regenerative agents to promote neuronal growth. We grew neuroblastoma cells on surfaces coated with AgNPs and studied the effect on the development of the neurites during the initiation and the elongation growth phases. We find that the AgNPs function as favorable anchoring sites, and the growth on the AgNP-coated substrates leads to a significantly enhanced neurite outgrowth. Cells grown on substrates coated with AgNPs have initiated three times more neurites than cells grown on uncoated substrates, and two times more than cells grown on substrates sputtered with a plain homogenous layer of silver. The growth of neurites on AgNPs in the elongation phase was enhanced as well. A comparison with substrates coated with gold nanoparticles (AuNPs) and zinc oxide nanoparticles (ZnONPs) demonstrated a clear silver material-driven promoting effect, in addition to the nanotopography. The growth on substrates coated with AgNPs has led to a significantly higher number of initiating neurites when compared to substrates coated with AuNPs or ZnONPs. All nanoparticle-coated substrates affected and promoted the elongation of neurites, with a significant positive maximal effect for the AgNPs. Our results, combined with the well-known antibacterial effect of AgNPs, suggest the use of AgNPs as an attractive nanomaterial - with dual activity - for neuronal repair studies.
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Silver nanoparticles enhance differentiation of SH-SY5Y cells.
“Enhance the differentiation of SH-SY5Y cells”
From Nanotechnology: A Promising Approach for Delivery of Neuroprotective Drugs (2020) · cites this paper as Alon et al., 2014
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Cites
Tissue Cells Feel and Respond to the Stiffness of Their Substrate
Discher, D. E., Janmey, P., & Wang, Y.-L. (2005) · Science · doi:10.1126/science.1116995 · Cited by 6,382 · see citing works
Silver nanoparticles as a new generation of antimicrobials
Rai, M., Yadav, A., & Gade, A. (2008) · Biotechnology Advances · doi:10.1016/j.biotechadv.2008.09.002 · Cited by 6,182 · see citing works
Antimicrobial effects of silver nanoparticles
Kim, J. S., Kuk, E., Yu, K. N., Kim, J., Park, S. J., Lee, H.-J., Ys, K., Park, Y. K., Park, Y. H., Hwang, C., et al. (2007) · Nanomedicine Nanotechnology Biology and Medicine · doi:10.1016/j.nano.2006.12.001 · Cited by 4,934 · see citing works
Silver nanoparticles: Green synthesis and their antimicrobial activities
Sharma, V. K., Yngard, R. A., & Lin, Y. (2008) · Advances in Colloid and Interface Science · doi:10.1016/j.cis.2008.09.002 · Cited by 3,972 · see citing works
Cytotoxicity and Genotoxicity of Silver Nanoparticles in Human Cells
Asharani, P. V., Low, G. K. M., Hande, M. P., & Valiyaveettil, S. (2008) · ACS Nano · doi:10.1021/nn800596w · Cited by 3,714 · see citing works
Raghupathi, K. R., Koodali, R. T., & Manna, A. C. (2011) · Langmuir · doi:10.1021/la104825u · Cited by 2,069 · see citing works
Design and validation of a tool for neurite tracing and analysis in fluorescence microscopy images
Meijering, E. H. W., Jacob, M., Sarria, J. ‐. F., Steiner, P., Hirling, H., & Unser, M. A. (2004) · Cytometry Part A · doi:10.1002/cyto.a.20022 · Cited by 1,611 · see citing works
Using sonochemistry for the fabrication of nanomaterials
Gedanken, A. (2004) · Ultrasonics Sonochemistry · doi:10.1016/j.ultsonch.2004.01.037 · Cited by 1,221 · see citing works
Self-citationTopical Delivery of Silver Nanoparticles Promotes Wound Healing
Tian, J., Wong, K. K., Ho, C., Lok, C., Yu, W., Che, C., Chiu, J., & Tam, P. K. H. (2006) · ChemMedChem · doi:10.1002/cmdc.200600171 · Cited by 984 · see citing works
Anti-proliferative activity of silver nanoparticles
Asharani, P. V., Hande, M. P., & Valiyaveettil, S. (2009) · BMC Cell Biology · doi:10.1186/1471-2121-10-65 · Cited by 663 · see citing works
Cytotoxicity and Immunological Response of Gold and Silver Nanoparticles of Different Sizes
Yen, H., Hsu, S., & Tsai, C. (2009) · Small · doi:10.1002/smll.200900126 · Cited by 635 · see citing works
Théry, M., Racine, V., Piel, M., Pépin, A., Dimitrov, A., Chen, Y., Sibarita, J., & Bornens, M. (2006) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0609267103 · Cited by 593 · see citing works
Hernández-Sierra, J. F., Ruíz, F., Pena, D. C. C., Martínez‐Gutiérrez, F., Martínez, A. E., Pozos‐Guillén, A., Tapia-Pérez, H., & Martínez-Castañón, G. A. (2008) · Nanomedicine Nanotechnology Biology and Medicine · doi:10.1016/j.nano.2008.04.005 · Cited by 560 · see citing works
Perelshtein, I., Applerot, G., Perkas, N., et al. (2008) · Nanotechnology · doi:10.1088/0957-4484/19/24/245705 · Cited by 442 · see citing works
Wright, J. B., Lam, K., Buret, A. G., Olson, M. E., & Burrell, R. E. (2002) · Wound Repair and Regeneration · doi:10.1046/j.1524-475x.2002.10308.x · Cited by 395 · see citing works
Liu, X., Lee, P., Ho, C., Lui, V. C. H., Chen, Y., Che, C., Tam, P. K. H., & Wong, K. K. (2010) · ChemMedChem · doi:10.1002/cmdc.200900502 · Cited by 336 · see citing works
Neurons sense nanoscale roughness with nanometer sensitivity
Brunetti, V., Maiorano, G., Rizzello, L., Sorce, B., Sabella, S., Cingolani, R., & Pompa, P. P. (2010) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0914456107 · Cited by 255 · see citing works
Mussel-inspired silver-releasing antibacterial hydrogels
Fullenkamp, D. E., Rivera, J. G., Gong, Y., Lau, K. H. A., He, L., Varshney, R., & Messersmith, P. B. (2012) · Biomaterials · doi:10.1016/j.biomaterials.2012.02.027 · Cited by 240 · see citing works
The influence of microchannels on neurite growth and architecture
Mahoney, M. J., Chen, R. R., Tan, J., & Saltzman, W. M. (2004) · Biomaterials · doi:10.1016/j.biomaterials.2004.03.015 · Cited by 226 · see citing works
Topographically modified surfaces affect orientation and growth of hippocampal neurons
Dowell‐Mesfin, N., Abdul-Karim, M., Turner, A. M. P., Schanz, S. J., Craighead, H. G., Roysam, B., Turner, J. N., & Shain, W. G. (2004) · Journal of Neural Engineering · doi:10.1088/1741-2560/1/2/003 · Cited by 214 · see citing works
Noninvasive Neuron Pinning with Nanopillar Arrays
Xie, C., Hanson, L., 谢文俊, Lin, Z. C., Cui, B., & Cui, Y. (2010) · Nano Letters · doi:10.1021/nl101950x · Cited by 189 · see citing works
Surface Nanopatterning to Control Cell Growth
Richert, L., Vetrone, F., Yi, J.-H., Zalzal, S., Wuest, J. D., Rosei, F., & Nanci, A. (2008) · Advanced Materials · doi:10.1002/adma.200701428 · Cited by 181 · see citing works
The antithrombotic and antimicrobial properties of PEG-protected silver nanoparticle coated surfaces
Unnithan, R. V. M., Unnikrishnan, S., Krishnan, V. K., et al. (2012) · Biomaterials · doi:10.1016/j.biomaterials.2012.01.005 · Cited by 118 · see citing works
Schikorski, D., Cuvillier‐Hot, V., Leippe, M., et al. (2008) · The Journal of Immunology · doi:10.4049/jimmunol.181.2.1083 · Cited by 103 · see citing works
Kang, K., Choi, S., Jang, H. S., Cho, W. K., Nam, Y., Choi, I. S., & Lee, J. S. (2011) · Angewandte Chemie International Edition · doi:10.1002/anie.201106271 · Cited by 98 · see citing works
Textural guidance cues for controlling process outgrowth of mammalian neurons
Hanson, J. N., Motala, M. J., Heien, M. L., et al. (2008) · Lab on a Chip · doi:10.1039/b803595d · Cited by 87 · see citing works
Axonal guidance on patterned free-standing nanowire surfaces
Prinz, C. N., Hällström, W., Mårtensson, T., Samuelson, L., Montelius, L., & Kanje, M. (2008) · Nanotechnology · doi:10.1088/0957-4484/19/34/345101 · Cited by 85 · see citing works
Topographic cues of nano‐scale height direct neuronal growth pattern
Baranes, K., Chejanovsky, N., Alon, N., et al. (2012) · Biotechnology and Bioengineering · doi:10.1002/bit.24444 · Cited by 81 · see citing works
Depositing silver nanoparticles on/in a glass slide by the sonochemical method
Perkas, N., Amirian, G., Applerot, G., et al. (2008) · Nanotechnology · doi:10.1088/0957-4484/19/43/435604 · Cited by 60 · see citing works
Buttiglione, M., Vitiello, F., Sardella, E., et al. (2007) · Biomaterials · doi:10.1016/j.biomaterials.2007.02.022 · Cited by 60 · see citing works
An in vitro injury model for SH-SY5Y neuroblastoma cells: Effect of strain and strain rate
Skotak, M., Wang, F., & Chandra, N. (2012) · Journal of Neuroscience Methods · doi:10.1016/j.jneumeth.2012.01.001 · Cited by 47 · see citing works
Topographic control of the growth and function of cardiomyoblast H9c2 cells using nanodot arrays
Pan, H.-A., Hung, Y., Sui, Y.-P., & Huang, G. S. (2011) · Biomaterials · doi:10.1016/j.biomaterials.2011.09.054 · Cited by 46 · see citing works
Baranes, K., Kollmar, D., Chejanovsky, N., et al. (2012) · Journal of Molecular Histology · doi:10.1007/s10735-012-9422-2 · Cited by 41 · see citing works
Observation of Growth of Human Fibroblasts on Silver Nanoparticles
Wen, H., Lin, Y.-N., Jian, S., et al. (2007) · Journal of Physics Conference Series · doi:10.1088/1742-6596/61/1/089 · Cited by 40 · see citing works
Topographical guidance of mouse neuronal cell on SiO2 microtracks
Lee, J. W., Lee, K., Cho, N., et al. (2007) · Sensors and Actuators B Chemical · doi:10.1016/j.snb.2007.06.017 · Cited by 32 · see citing works
The Influence of Porous Silicon on Axonal Outgrowth in Vitro
Johansson, F., Kanje, M., Linsmeier, C. E., et al. (2008) · IEEE Transactions on Biomedical Engineering · doi:10.1109/tbme.2007.912423 · Cited by 27 · see citing works
Kang, K., Choi, S., Jang, H. S., et al. (2011) · Angewandte Chemie · doi:10.1002/ange.201106271 · Cited by 14 · see citing works
Flagged first, then by citations
Cited by
Sorted by citations
Nanotechnology: A Promising Approach for Delivery of Neuroprotective Drugs
Naqvi, S., Panghal, A., & Flora, S. J. S. (2020) · Frontiers in Neuroscience · doi:10.3389/fnins.2020.00494 · Cited by 305 · see citing works
Nikolov, A. S., Balchev, I. I., Nedyalkov, N. N., Kostadinov, I. K., Karashanova, D. B., & Atanasova, G. B. (2017) · Applied Physics A · doi:10.1007/s00339-017-1328-0 · Cited by 22 · see citing works
- Authors
- Shefi, O., Alon, Miroshnikov, Perkas, Gedanken, A., & Nissan, I.
- Affiliations
- Bar-Ilan University (IL)
- Corresponding author
- Shefi, Orit
- Venue
- International Journal of Nanomedicine
- Publisher
- Informa UK Limited
- Published
- 2014-05
- Volume
- 9 Suppl 1
- Pages
- 23
- ISSN
- 1176-9114 (ISSN-L)
- 1178-2013
- PMC
- PMC4024974
- OpenAlex
- W2142364296
- Cited by
- 47
- References
- 37
- Citations by year
- 2025: 3 · 2024: 3 · 2023: 2 · 2022: 4 · 2021: 2 · 2020: 5 · 2019: 5 · 2018: 8 · 2017: 5 · 2016: 3 · 2015: 5 · 2014: 2
- FWCI
- 2.28
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- International Journal of Nanomedicine (publishedVersion, cc-by-nc)
- Europe PMC (PubMed Central) (publishedVersion, cc-by-nc)
- PubMed Central (publishedVersion, cc-by-nc)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Dove Medical Press (Taylor and Francis Group) (submittedVersion, cc-by-nc)
- Topics
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Nerve injury and regeneration
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neural Engineering
- Physical Sciences › Materials Science › Materials Chemistry › Nanoparticles: synthesis and applications
- Keywords
- silver nanoparticles, neuronal regeneration, neurite outgrowth, neuroblastoma cells, gold nanoparticles, zinc oxide nanoparticles, nanotopography, antibacterial activity
- MeSH terms
- Anti-Bacterial Agents, Cell Survival, Humans, Nerve Regeneration, Silver, Neurites, Cell Line, Tumor, Cell Proliferation, Nanomedicine, Metal Nanoparticles
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
