A highly sensitive chemiluminescence assay for superoxide detection and chronic granulomatous disease diagnosis
Open accessAbstract
Reactive oxygen species (ROS) produced by neutrophils are crucial for defense against infectious diseases, and the adequate measurement of ROS levels is an important way to evaluate the possibility of infections. The fluorescent probe dihydrorhodamine 123 has been applied exclusively to the measurement of ROS thus far. We developed a novel method for detecting ROS, which utilizes the chemiluminescent probes Luminol and Diogenes. The new method quantitatively detects ROS produced by as few as 10 to 10(4) neutrophils. Furthermore, this method can detect ROS levels in one microliter of whole blood or ROS produced by Epstein-Barr immortalized B lymphocytes. This method will be valuable for prompt diagnosis of neonatal chronic granulomatous diseases in which neutrophils aberrantly produce superoxide.
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The neutrophil respiratory burst is usually measured spectrophotometrically by reduction of ferricytochrome C to ferrocytochrome C with absorbance at 550 nm, or histologically by reduction of nitro blue tetrazolium to insoluble formazan.
“The neutrophil respiratory burst is usually measured spectrophotometrically: (a) following the reduction of ferricytochrome C Fe3+ which is reduced by superoxide, producing oxygen and bright-red ferrocytochrome C Fe2+ with a detectable spectrophotometric absorbance at 550 nm [152,153]; and (b) histologically using the tetrazolium salt, nitro blue tetrazolium, which is reduced intracellularly to an”
From Superoxide Anion Chemistry—Its Role at the Core of the Innate Immunity (2023) · cites this paper as [153]
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Cites
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Superoxide Anion Chemistry—Its Role at the Core of the Innate Immunity
Andrés, C. M. C., Pérez de la Lastra, J. M., Andrés Juan, C., Plou, F. J., & Pérez-Lebeña, E. (2023) · International Journal of Molecular Sciences · doi:10.3390/ijms24031841 · Cited by 342 · see citing works
Saha, S., Basu, M., Guin, S., Gupta, P., Mitterstiller, A.-M., Weiss, G., Jana, K., & Ukil, A. (2019) · The Journal of Immunology · doi:10.4049/jimmunol.1800958 · Cited by 47 · see citing works
- Authors
- Yamazaki, T., Kawai, C., Yamauchi, A., & Kuribayashi, F.
- Affiliations
- Tokai University (JP)
- Kawasaki Medical School (JP)
- Corresponding author
- Kuribayashi, Futoshi
- ORCID
- Kuribayashi, Futoshi
- Venue
- Tropical Medicine and Health
- Published
- 2011
- Volume
- 39
- Issue
- 2
- Pages
- 41-45
- ISSN
- 1348-8945 (ISSN-L)
- 1349-4147
- PMC
- PMC3153160
- OpenAlex
- W2034035329
- Cited by
- 38
- References
- 16
- Citations by year
- 2026: 3 · 2024: 1 · 2023: 3 · 2022: 5 · 2021: 4 · 2020: 3 · 2019: 2 · 2018: 6 · 2017: 5 · 2016: 1 · 2014: 2 · 2013: 1 · 2012: 2
- FWCI
- 0.71
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Tropical Medicine and Health (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Ministry of Education, Culture, Sports, Science and Technology
- Kawasaki Medical School
- Japan Society for the Promotion of Science (21591365)
- Topics
- Life Sciences › Immunology and Microbiology › Immunology › Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › bioluminescence and chemiluminescence research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Redox biology and oxidative stress
- Keywords
- superoxide detection, chemiluminescence assay, chronic granulomatous disease, reactive oxygen species, neutrophils, luminol, Diogenes, dihydrorhodamine 123, neonatal diagnosis, Epstein-Barr virus-transformed B lymphocytes
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