Molecular Genetic Studies on (Ficus Palmata & Ficus Carica Subsp Rupestris.) Under the Effect of Gamma Radiation by Tissue Culture Techniques
Open accessAbstract
Ficus spp. are used as medicine and are used to reducing the risk of cancer and heart disease. The phytochemical screening of the Ficus palmata plant extracts showed the presence of alkaloids, tannins, flavonoids, terpenoids and cardiac glycosides. This investigation was undertaken by using tissue culture technique on Ficus palmata & Ficus carica subsp rupestris. Study the effect of gamma rays doses of 0.0, 20, 30 and 40 Gy on callus production and the secondary plant constituents. The highest callus fresh weightand callus induction were achieved by callus derived from (leaves & stem) explants, which were cultured on (MS) medium supplemented with different concentrations of 2,4-D at 2.0 (mg/l) and Kin at 0.2 (mg/l). Polymerase chain reaction (PCR) amplification using eight ISSR primers showed polymorphic patterns with different response to gamma radiation doses. found genetic differences at the molecular level using ISSR with gamma doses compared to control. The total number of polymorphic bands was 239 bands (99.58% polymorphism). Found that there was genetic stability between the mother plants and product tissue culture for plants (Ficus palmata & Ficus carica subsp rupestris). The chemical composition of Ficus palmata was studied by means of GC-MS analysis under the effect of gamma radiation. The best doses of gamma were 30 & 40 Gy, as they increased the secondary compounds of Ficus palmata.
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Physical and chemical factors such as light intensity, temperature, and toxic substances can induce genetic changes.
“Physical and chemical factors such as light intensity, temperature, and toxic substances can induce genetic changes [78].”
From Somaclonal Variation and Clonal Fidelity in Commercial Micropropagation: Challenges and Perspectives (2025) · cites this paper as [78]
The “Trying to produce new genotypes using mutagens” and “Molecular genetic marker by ISSR” sections describe gamma irradiation of fig tissue and genetic differences associated with gamma-ray doses, supporting the point that a physical factor can induce genetic changes. The cited paper does not establish the claim for light intensity, temperature, or toxic substances, nor for chemical factors generally. Page numbers are unavailable in our copy. (LLM, conf=0.85)
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Somaclonal Variation and Clonal Fidelity in Commercial Micropropagation: Challenges and Perspectives
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- Authors
- Eissa, S. S., Sayed, A. E., M. M. Abd Allah, M. M., & EL-shaer, H. F.
- Affiliations
- Al-Azhar University (EG)
- ORCID
- EL-shaer, H. F.
- Venue
- Al-Azhar Journal of Agricultural Research
- Published
- 2021-12-01
- Volume
- 46
- Issue
- 2
- Pages
- 128-138
- ISSN
- 1110-1563 (ISSN-L)
- 2786-0051
- OpenAlex
- W4285325580
- Cited by
- 2
- References
- 14
- Citations by year
- 2026: 1 · 2024: 1
- FWCI
- 0.15
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Al-Azhar Journal of Agricultural Research (publishedVersion)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Phytochemistry and biological activities of Ficus species
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Plant tissue culture and regeneration
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Genetic and Mutation Studies
- Keywords
- Ficus palmata, gamma radiation, callus induction, ISSR markers, genetic polymorphism, genetic stability, phytochemical composition, secondary metabolites, 2,4-D, kinetin, medicinal plants
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