DEVELOPMENT OF IN VITRO SOMACLONAL VARIATION FOR PELARGONIUM GRAVEOLENS L’HERIT, AIT
Open accessAbstract
In vitro somaclonal variation via callus is considered as one of the most effective tool for improvement of vegetative propagated crops. Different combinations of cytokinine (BA) and auxine (NAA) were used to induce somaclonal variation from leaf explants of the Egyptian geranium cultivar, Pelargonium graveolens L’Herit, Ait. El Qanater El khayria cv.8. Callus induction and regenerated shoots from callus were evaluated; 2.5 mg/L BA and 0.5 mg/L NAA was the optimal for callus induction and number of regenerated shoots. The obtained plantlets were acclimatized under glasshouse conditions with 80% to 90% survival and were transferred to the open field. The evaluation of growth traits and oil analysis were done for the new 13 clones compared with the mother plant in randomized block design for two successive seasons. Characterization of these new clones for morphological traits and essential oil yield and quality demonstrated the induction of variability in negative and positive directions in some characteristics in comparison with the mother plant. Clone no.7 was the superior clone in herb 2264.7 & 2357.3 g/plant and oil yield 2.436 & 3.065 ml/plant in first and second season, respectively, than the mother plant and other new clones with acceptable content of citronellol 25.63% and geraniol 26.52% as a parameter for volatile oil quality. The genetic variation of the new geranium clones were tested using SRAP technique based on PCR, the polymorphism was detected using 16 pairs of SRAP primers in combinations as forward and reverse. The genetic differences between new clones and the mother plant were ascertained with SRAP primer pair (me2 em4); a unique profile for clone no.7 was detected in SRAP analysis. The similarity percentage varied between 66.7% to 100% homologies among the new clones and their mother plant. The presence of genetic variation between new clones and the mother plant proved that the calliclones were somaclonal mutants, and the geranium cultivar, El Qanater El khayria cv.8, was improved for high yield in herb and essential oil.
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In Pelargonium graveolens, somaclones obtained by in vitro regeneration via callus had superior volatile oil (citronellol and geraniol) contents than the mother plant.
“For example, Hariedy et al., 2019 [145], obtained, by in vitro regeneration via callus, Pelargonium graveolens L’Herit somaclones with superior volatile oil (citronellol and geraniol) contents than the mother plant;”
From Somaclonal Variation—Advantage or Disadvantage in Micropropagation of the Medicinal Plants (2023) · cites this paper as Hariedy et al., 2019 [145]
The Abstract reports that the somaclones were regenerated via callus and that clone 7 had a higher oil yield than the mother plant. But the ‘Oil composition of new geranium clones’ section says geraniol content was lower in every new clone (26.5–30.5% versus 33.22% in the mother plant); citronellol content varied, with some clones exceeding the mother plant. The paper therefore does not support describing both constituents as superior. (LLM, conf=0.90)
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- Amar, M.H., Biswas M.K., Zhang Z and Guo W- K. 2011. Exploitation of SSR, SRAP and CAPS-SNP markers for genetic diversity of cit- rus germplasm collection. Scientia Horticul- turae 128, 220-227. Arab Republic of Egypt, Ministry of Agriculture. and Land Reclamation Economic Affairs, Development of in vitro somaclonal variation for Pelargonium graveolens L'Herit, Ait AUJAS, Ain Shams Univ., Cairo, Egypt, Special Issue, 27(1), 2019 575
- Sector, 2016, Bulletin of Agriculture Statis- tics, Part 1, Winter Crops, 2014-2015, 92 p. Cairo, Egypt. British Pharmacopoeia 1963. The Pharmaceuti- cal Press Bloomsburg, 17, 112-125, Square.
- Dunbar, R.R. 1997. In: Advances in Agronomy. Tissue culture induced variations and crop im- provement, Academic Press, New York, USA, Ch. 58, pp. 201-240.
- Egyptian Pharmacopoeia. 2005. The English Text of Arab Republic of Egypt, 4 th ed., Cen- tral Adminstration and Pharmaceutical Affairs (CAPA). Ministry of Health and Population: Cai- ro, Egypt, pp. 31-33.
- Evans, D.A. and W.R. Sharp. 1986. Applications of Somaclonal Variation_Bio/Technology, 4(6), 528-532.
- Gauri, S., S. Banerjee, L. Rahman, G.R. Mallava- rapu, S. Sharma, Sushil-Kumar and S. Ku- mar. 2000. An effentiant in vitro procedure for micropropagation and generation of somaclones of rose scented Pelargonium. Plant Science Limerick.155(2), 133-140.
- Gulati, B.C. 1960. Cultivation of geranium. Ind. Oil Soap J. 26(2), 35-43.
- Gupta, Ritika, S. Banerjee, G.R. Mallavarapu, S. Sharma, S.P.S Khanuja, A.K. Shasany and Sushil Kumar¹. 2002. Development of a supe- rior somaclone of Rose-scented geranium and a protocol for inducing variants. HortScience. 37(4), 632-636.
- Harridy, I.M.A., El-Zahwey, A.M.H., Soliman, S.G.I. and El-Taweel, S.M.I..1998. Geranium cultivars with a high herb and oil productivity. J. Agric. Sci., Mansoura Univ., 23(6), 2687- 2697.
- Korir, Nicholas Kibet, Jian Han, Lingfei Shangguan, Chen Wang, Emrul Kayesh, Yanyi Zhang, and Jinggui Fang. 2013. Plant variety and cultivar identification: advances and prospects. Critical Reviews in Biotechnolo- gy, 33(2), 111-125.
- Qureshi, J.A. and P.K. Saxena. 1992. Adventi- tious shoot induction and somatic embryogen- esis with intact seedlings of several hybrid seed Rasha Hariedy, Rashed and Mahassen Sidky AUJAS, Ain Shams Univ., Cairo, Egypt, Special Issue, 27(1), 2019
- Rajeswara, Rao, Prakasa Rao, B.R. and Narya- na M.R.. 1989. Rose geranium: An economical crop in the south Indian Plains. Ind. Hortic. 36(2), 14-17. , A , Soliman K.M., Jorgensen R. Am. and Allard R.W.L.1984.Ribosomal DNA spacer-length polymorphisms in barley: Mendelian inheritance, chromosomal location and population dynamics. Proc. Nat. Academy of Science. 81(24), 8014-8018.
- th Ed. Iowa State College Press, Ames. Iowa, U.S. A. Teisseire, P., 1987. Industerial quality control of essential oils by capillary GC. In: Sandra P. and Bicchi C. (Ed.), Capillary Gas Chroma- tography in Essential Oil Analysis. Hue- thig, Heidelberg, pp. 215-258.
- Tembe, Rashmi P. and Manjushari A. Deodhar. 2010. Chemical and Molecular fingerprinting of different Pelargonium graveolens L'Herit. Viz., Reunion, Bourbon and Egyptian. Biotechnol- ogy. 9(4), 485-491.
- Veilleux, R.E. and Johnson, A.A.T. 1998. Somaclonal variations: Molecular analysis, transformation interaction and utilization. In: Janick J. (ed.). Plant Breeding Rev., 16, 229- 268..
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Somaclonal Variation—Advantage or Disadvantage in Micropropagation of the Medicinal Plants
Duta-Cornescu, G., Constantin, N., Pojoga, D.-M., Nicuta, D., & Simon-Gruita, A. (2023) · International Journal of Molecular Sciences · doi:10.3390/ijms24010838 · Cited by 108 · see citing works
- Authors
- Hariedy, R., Rashed, M., & Sidky, M.
- Affiliations
- Agricultural Research Center (EG)
- Ain Shams University (EG)
- ORCID
- Rashed, M.
- Venue
- Arab Universities Journal of Agricultural Sciences
- Published
- 2019-03-01
- Volume
- 27
- Issue
- 1
- Pages
- 563-577
- ISSN
- 1110-2675 (ISSN-L)
- 2636-3585
- OpenAlex
- W2969744203
- Cited by
- 1
- References
- 0
- Citations by year
- 2023: 1
- FWCI
- 0.00
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- cc-by-nc-sa
- Locations
- Arab Universities Journal of Agricultural Sciences (publishedVersion, cc-by-nc-sa)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Plant tissue culture and regeneration
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Flowering Plant Growth and Cultivation
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Genetic and Mutation Studies
- Keywords
- somaclonal variation, Pelargonium graveolens, Geranium, callus induction, leaf explants, essential oil yield, citronellol, geraniol, SRAP markers, genetic variation, herb yield, acclimatization
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