Scanning electron microscopic changes in Habronema muscae after in vitro exposure to plant extract (Verbesina alternifolia)
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Scanning electron microscopy (SEM) was used to study the morphological alteration occurring in Habronema muscae adult female worms after in vitro exposure to different doses of Verbesina alternifolia oil extract. The half maximal lethal concentration (LC50) was reached 400 ppm after 24hrs, while LC100 was reached 600 ppm after 48hrs. Irreversible degenerative changes were recorded such as shrinking, detachment and distortion of the cuticle, cephalic and distal region. The cuticular surface had a wrinkled, corrugated appearance with longitudinal ridges and transverse thick folds. The lips and papillae were deformed and aggregated over each other. There is a direct relation between the level of the recorded degenerative changes and the increase in the dose and exposure time. In the same time no degen'erative changes were recorded in the control worm exposed to PBS till the end of the exposure period.
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The recent in vitro experiment on anthelmintic properties of Verbesina alternifolia against H. muscae demonstrated by SEM an irreversible degenerative change of the treated worm.
“The recent in vitro experiment on anthelmintic properties of Verbesina alternifolia (crown beard) against H. muscae which demonstrated by SEM an irreversible degenerative change of the treated worm (80)”
From Habronematidosis in Equids: Current Status, Advances, Future Challenges (2020) · cites this paper as [80]
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Habronematidosis in Equids: Current Status, Advances, Future Challenges
Barlaam, A., Traversa, D., Papini, R., & Giangaspero, A. (2020) · Frontiers in Veterinary Science · doi:10.3389/fvets.2020.00358 · Cited by 34 · see citing works
- Authors
- ABDEL-WAHAB, A., ABDEL-MAOGOOD, S., & MAHDY, O.
- Affiliations
- Cairo University (EG)
- ORCID
- MAHDY, O.
- Venue
- Journal of the Egyptian Society of Parasitology
- Published
- 2017-04-01
- Volume
- 47
- Issue
- 1
- Pages
- 47-54
- ISSN
- 1110-0583 (ISSN-L)
- 2090-2549
- OpenAlex
- W3024724759
- Cited by
- 5
- References
- 40
- Citations by year
- 2026: 1 · 2022: 2 · 2021: 1 · 2020: 1
- FWCI
- 0.43
- Type
- journal article
- Language
- en
- Open access
- diamond
- doi.org
- Licence
- cc-by
- Locations
- Journal of the Egyptian Society of Parasitology (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Topics
- Health Sciences › Veterinary › Small Animals › Helminth infection and control
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Insect Pest Control Strategies
- Health Sciences › Medicine › Infectious Diseases › Parasitic Diseases Research and Treatment
- Keywords
- Habronema muscae, Verbesina alternifolia, plant extract toxicity, adult female worms, dose-response relationship, LC50, insecticidal activity
- MeSH terms
- Animals, Egypt, Female, Horse Diseases, Horses, Lethal Dose 50, Microscopy, Electron, Scanning, Plant Extracts, Spiruroidea, Spirurida Infections, Equidae, Verbesina
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