Production of recombinant therapeutic proteins in human cells: current achievements and future perspectives
Abstract
Over the past 20 years the demand for recombinant proteins has increased significantly. Mammalian cell lines have been extensively used to produce recombinant proteins. This expression system offers several advantages over microbial systems, mammalian cells have the cellular machinery to promote the secretion of the recombinant product and the posttranslational modifications, like glycosylation that is present in many of recombinant therapeutic proteins in the market. Human cell lines have emerged as a new and powerful alternative for production of such products. These cells are able to produce recombinant proteins with posttranslational modifications more similar to their natural counterparts, producing proteins with human-like glycosylation pattern avoiding immunogenic reactions against epitopes nonhumans. This review presents the available human cell lines that can be used in pharmaceutical industry, the advantages of this expression system and the main efforts made in this field.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
HKB-11 cells have recently shown high-level protein production and α2,3 and α2,6-sialic acid linkages.
“It has recently showed high-level protein production and α2,3 and α2,6-sialic acid linkages (Picanco-Castro et al., 2013).”
From Therapeutic glycoprotein production in mammalian cells (2017) · cites this paper as Picanco-Castro et al., 2013
Cites
Large-Scale transfection of mammalian cells for the fast production of recombinant protein
Pham, P. L., Kamen, A., & Durocher, Y. (2006) · Molecular Biotechnology · doi:10.1385/mb:34:2:225 · Cited by 236 · see citing works
Flagged first, then by citations
Cited by
Sorted by citations
Therapeutic glycoprotein production in mammalian cells
Lalonde, M.-E., & Durocher, Y. (2017) · Journal of Biotechnology · doi:10.1016/j.jbiotec.2017.04.028 · Cited by 296 · see citing works
- Authors
- Picanco-Castro, V., Biaggio, R., Cova, D., & Swiech, K.
- Affiliations
- Universidade de São Paulo (BR)
- Venue
- Protein & Peptide Letters
- Publisher
- Bentham Science Publishers Ltd.
- Published
- 2013-11
- Volume
- 20
- Issue
- 12
- Pages
- 1373-1381
- ISSN
- 0929-8665 (ISSN-L)
- OpenAlex
- W1996524348
- Cited by
- 34
- References
- 1
- Citations by year
- 2025: 2 · 2023: 3 · 2022: 1 · 2021: 1 · 2020: 4 · 2019: 3 · 2018: 5 · 2017: 7 · 2016: 4 · 2015: 2 · 2014: 2
- FWCI
- 1.19
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Protein and Peptide Letters (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Fundação de Amparo à Pesquisa do Estado de São Paulo
- U.S. Food and Drug Administration
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Viral Infectious Diseases and Gene Expression in Insects
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biotechnology › Transgenic Plants and Applications
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein purification and stability
- Keywords
- recombinant therapeutic proteins, human cell lines, mammalian cell expression systems, post-translational modification, glycosylation, immunogenicity, recombinant protein expression, pharmaceutical manufacturing
- MeSH terms
- Biotechnology, Cell Line, Glycosylation, Humans, Protein Processing, Post-Translational, Recombinant Proteins, Bioreactors
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
